scholarly journals Identification of blue-green algal nitrogen fixation genes by using heterologous DNA hybridization probes

1980 ◽  
Vol 77 (1) ◽  
pp. 186-190 ◽  
Author(s):  
B. J. Mazur ◽  
D. Rice ◽  
R. Haselkorn
Keyword(s):  
Nitrogen Fixation ◽  
Dna Hybridization ◽  
Green Algal ◽  
Hybridization Probes ◽  
Nitrogen Fixation Genes

scholarly journals Physical map of the Saccharomyces cerevisiae genome at 110-kilobase resolution.

Genetics ◽  
1991 ◽  
Vol 127 (4) ◽  
pp. 681-698 ◽  
Author(s):  
A J Link ◽  
M V Olson
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Dna Hybridization ◽  
Physical Map ◽  
Single Copy ◽  
Restriction Endonucleases ◽  
Yeast Genome ◽  
Hybridization Probes ◽  
Copy Dna ◽  
Single Copy Dna

Abstract A physical map of the Saccharomyces cerevisiae genome is presented. It was derived by mapping the sites for two restriction endonucleases, SfiI and NotI, each of which recognizes an 8-bp sequence. DNA-DNA hybridization probes for genetically mapped genes and probes that span particular SfiI and NotI sites were used to construct a map that contains 131 physical landmarks--32 chromosome ends, 61 SfiI sites and 38 NotI sites. These landmarks are distributed throughout the non-rDNA component of the yeast genome, which comprises 12.5 Mbp of DNA. The physical map suggests that those genes that can be detected and mapped by standard genetic methods are distributed rather uniformly over the full physical extent of the yeast genome. The map has immediate applications to the mapping of genes for which single-copy DNA-DNA hybridization probes are available.

Expression of the nodulation and nitrogen fixation genes in Rhizobium meliloti during development

Genome ◽  
1989 ◽  
Vol 31 (1) ◽  
pp. 354-360 ◽  
Author(s):  
San Chiun Shen ◽  
Shui Ping Wang ◽  
Guan Qiao Yu ◽  
Jia Bi Zhu
Keyword(s):  
Nitrogen Fixation ◽  
Rhizobium Meliloti ◽  
Abnormal Development ◽  
Wild Type ◽  
Free Living ◽  
Nod Genes ◽  
Nodule Initiation ◽  
Fix Genes ◽  
Nitrogen Fixation Genes

Genes that specify nodulation (nod genes) are only active in the free-living rhizobia or in the nodule initiation state of rhizobia. As soon as the repression of nod genes occurs in the bacteroids of the nodule, nifA is induced, while ntrC is inactivated and thus the nifA-mediated nif/fix genes are turned on. Limitation of available oxygen brings about the induction of nifA, which reflects the actual status of nif/fix gene activities in symbiotic state of rhizobia. Oxygen thus appears to be a major symbiotic signal to the expression of bacteroid nif/fix genes. Mutation of nifA or shortage of nifA product in wild-type rhizobia caused by the inhibition of multicopy nifH/fixA promoters leads to an abnormal development of nodules and premature degradation of bacteroids in nodules.Key words: nitrogen fixation, nodulation, nif/fix regulation, nifA mutant.

scholarly journals Diversity of heterotrophic nitrogen fixation genes in a marine cyanobacterial mat.

1995 ◽  
Vol 61 (7) ◽  
pp. 2527-2532 ◽  
Author(s):  
J P Zehr ◽  
M Mellon ◽  
S Braun ◽  
W Litaker ◽  
T Steppe ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Cyanobacterial Mat ◽  
Nitrogen Fixation Genes

The Nitrogen Fixation Genes

Nature ◽  
1972 ◽  
Vol 239 (5374) ◽  
pp. 495-499 ◽  
Author(s):  
STANLEY L. STREICHER ◽  
ELIZABETH G. GURNEY ◽  
RAYMOND C. VALENTINE
Keyword(s):  
Nitrogen Fixation ◽  
Nitrogen Fixation Genes
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