scholarly journals Specific 5' flanking sequences are required for faithful initiation of in vitro transcription of the ovalbumin gene

1981 ◽  
Vol 78 (2) ◽  
pp. 879-883 ◽  
Author(s):  
S. Y. Tsai ◽  
M.-J. Tsai ◽  
B. W. O'Malley
Keyword(s):  
In Vitro Transcription ◽  
Flanking Sequences

5′-flanking sequences that inhibit in vitro transcription of a Xenopus laevis tRNA gene

Cell ◽  
1983 ◽  
Vol 34 (3) ◽  
pp. 881-890 ◽  
Author(s):  
Robert A. Hipskind ◽  
Stuart G. Clarkson
Keyword(s):  
Xenopus Laevis ◽  
Trna Gene ◽  
In Vitro Transcription ◽  
Flanking Sequences

A discrete region centered 22 base pairs upstream of the initiation site modulates transcription of Drosophila tRNAAsn genes

1988 ◽  
Vol 8 (10) ◽  
pp. 4441-4449
Author(s):  
A K Lofquist ◽  
A D Garcia ◽  
S J Sharp
Keyword(s):  
Transcription Initiation ◽  
In Vitro Transcription ◽  
Initiation Site ◽  
Trna Genes ◽  
Selection Mechanism ◽  
Base Pairs ◽  
Discrete Region ◽  
Flanking Sequences ◽  
Actual Sequence

We have studied the mechanism by which 5'-flanking sequences modulate the in vitro transcription of eucaryotic tRNA genes. Using deletion and linker substitution mutagenesis, we have found that the 5'-flanking sequences responsible for the different in vitro transcription levels of three Drosophila tRNA5Asn genes are contained within a discrete region centered 22 nucleotides upstream from the transcription initiation site. In conjunction with the A-box intragenic control region, this upstream transcription-modulatory region functions in the selection mechanism for the site of transcription initiation. Since the transcription-modulatory region directs the position of the start site and the actual sequence of the transcription-modulatory region determines the level of tRNAAsn gene transcription, the possibility is raised that the transcription-modulatory region directs a transcription initiation event similar to open complex formation at procaryotic promoters.

scholarly journals A discrete region centered 22 base pairs upstream of the initiation site modulates transcription of Drosophila tRNAAsn genes.

1988 ◽  
Vol 8 (10) ◽  
pp. 4441-4449 ◽  
Author(s):  
A K Lofquist ◽  
A D Garcia ◽  
S J Sharp
Keyword(s):  
Transcription Initiation ◽  
In Vitro Transcription ◽  
Initiation Site ◽  
Trna Genes ◽  
Selection Mechanism ◽  
Base Pairs ◽  
Discrete Region ◽  
Flanking Sequences ◽  
Actual Sequence

We have studied the mechanism by which 5'-flanking sequences modulate the in vitro transcription of eucaryotic tRNA genes. Using deletion and linker substitution mutagenesis, we have found that the 5'-flanking sequences responsible for the different in vitro transcription levels of three Drosophila tRNA5Asn genes are contained within a discrete region centered 22 nucleotides upstream from the transcription initiation site. In conjunction with the A-box intragenic control region, this upstream transcription-modulatory region functions in the selection mechanism for the site of transcription initiation. Since the transcription-modulatory region directs the position of the start site and the actual sequence of the transcription-modulatory region determines the level of tRNAAsn gene transcription, the possibility is raised that the transcription-modulatory region directs a transcription initiation event similar to open complex formation at procaryotic promoters.

In vitro transcription and translation of simian rotavirus SA11 gene products.

1980 ◽  
Vol 33 (3) ◽  
pp. 1111-1121 ◽  
Author(s):  
B B Mason ◽  
D Y Graham ◽  
M K Estes
Keyword(s):  
In Vitro Transcription ◽  
Gene Products
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