scholarly journals Point mutants of Moloney murine leukemia virus that fail to package viral RNA: evidence for specific RNA recognition by a "zinc finger-like" protein sequence.

1988 ◽  
Vol 85 (22) ◽  
pp. 8420-8424 ◽  
Author(s):  
R. J. Gorelick ◽  
L. E. Henderson ◽  
J. P. Hanser ◽  
A. Rein
Keyword(s):  
Zinc Finger ◽  
Protein Sequence ◽  
Murine Leukemia Virus ◽  
Leukemia Virus ◽  
Viral Rna ◽  
Moloney Murine Leukemia Virus ◽  
Rna Recognition ◽  
Murine Leukemia

scholarly journals Viral RNA annealing activities of the nucleocapsid protein of Moloney murine leukemia virus are zinc independent

1991 ◽  
Vol 19 (13) ◽  
pp. 3533-3541 ◽  
Author(s):  
Anne-Catherine Prats ◽  
Valérie Housset ◽  
Gé de Billy ◽  
Fabrice Cornille ◽  
Hervé Prats ◽  
...  
Keyword(s):  
Murine Leukemia Virus ◽  
Nucleocapsid Protein ◽  
Leukemia Virus ◽  
Viral Rna ◽  
Moloney Murine Leukemia Virus ◽  
Murine Leukemia

Investigation of zinc-binding affinities of moloney murine leukemia virus nucleocapsid protein and its related zinc finger and modified peptides

Biopolymers ◽  
1991 ◽  
Vol 31 (7) ◽  
pp. 899-906 ◽  
Author(s):  
Yves Mely ◽  
Fabrice Cornllle ◽  
Marie-Claude Fournié-Zaluski ◽  
Jean-Luc Darlix ◽  
Bernard F. Roques ◽  
...  
Keyword(s):  
Zinc Finger ◽  
Murine Leukemia Virus ◽  
Nucleocapsid Protein ◽  
Leukemia Virus ◽  
Moloney Murine Leukemia Virus ◽  
Zinc Binding ◽  
Binding Affinities ◽  
Modified Peptides ◽  
Murine Leukemia

scholarly journals Identification of Residues of the Moloney Murine Leukemia Virus Nucleocapsid Critical for Viral DNA Synthesis In Vivo

2001 ◽  
Vol 75 (6) ◽  
pp. 2616-2626 ◽  
Author(s):  
Jason Gonsky ◽  
Eran Bacharach ◽  
Stephen P. Goff
Keyword(s):  
Murine Leukemia Virus ◽  
Leukemia Virus ◽  
Viral Rna ◽  
Moloney Murine Leukemia Virus ◽  
Single Amino Acid ◽  
Primer Binding Site ◽  
Nucleic Acid Binding ◽  
Viral Dna ◽  
Murine Leukemia

ABSTRACT The nucleocapsid (NC) protein of retroviruses is a small nucleic acid-binding protein important in virion assembly and in the encapsidation of the viral RNA genome into the virion particle. Multiple single-amino-acid substitutions were introduced into the NC of Moloney murine leukemia virus to examine further its role in viral replication. Two residues were shown to play important roles in the early events of replication. Unlike viruses with previously characterized NC mutations, these viruses showed no impairment in the late events of replication. Viruses containing the substitutions L21A and K30A expressed the normal complement of properly processed viral Gag proteins. Analysis of the RNA content of mutant virions revealed normal levels of unspliced and spliced viral RNA, and the tRNAPro primer was properly annealed to the primer binding site on the viral genome. The virions demonstrated no defect in initiation of reverse transcription using the endogenous tRNA primer or in the synthesis of long viral DNA products in vitro. Nonetheless, viruses possessing these NC mutations demonstrated significant defects in the synthesis and accumulation of viral DNA products in vivo.

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