scholarly journals Spontaneous electrical activity of interstitial cells of Cajal isolated from canine proximal colon.

1989 ◽  
Vol 86 (18) ◽  
pp. 7280-7284 ◽  
Author(s):  
P. Langton ◽  
S. M. Ward ◽  
A. Carl ◽  
M. A. Norell ◽  
K. M. Sanders
Keyword(s):  
Electrical Activity ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Proximal Colon ◽  
Spontaneous Electrical Activity ◽  
Cells Of Cajal

Interstitial cells of Cajal and electrical activity in ganglionic and aganglionic colons of mice

2002 ◽  
Vol 283 (2) ◽  
pp. G445-G456 ◽  
Author(s):  
Sean M. Ward ◽  
Michael D. Gershon ◽  
Kathleen Keef ◽  
Yulia R. Bayguinov ◽  
Cheryl Nelson ◽  
...  
Keyword(s):  
Electrical Activity ◽  
Interstitial Cells Of Cajal ◽  
Circular Muscle ◽  
Interstitial Cells ◽  
Proximal Colon ◽  
Neural Responses ◽  
Wild Type ◽  
Circular Layer ◽  
Excitatory Junction Potentials ◽  
Cells Of Cajal

An antibody directed against Kit protein was used to investigate the distribution of interstitial cells of Cajal (ICC) within the murine colon. The ICC density was greatest in the proximal colon and decreased along its length. The distribution of the different classes of ICC in the aganglionic colons of lethal spotted ( ls/ls) mice was found to be similar in age-matched wild-type controls. There were marked differences in the electrical activities of the colons from ls/ls mutants compared with wild-type controls. In ls/ls aganglionic colons, the circular muscle was electrically quiescent compared with the spontaneous spiking electrical activity of wild-type tissues. In ls/ls aganglionic colons, postjunctional neural responses were greatly affected. Inhibitory junction potentials were absent or excitatory junction potentials inhibited by atropine were observed. In conclusion, the distribution of ICC in the ganglionic and aganglionic regions of the colons from ls/ls mutants appeared similar to that of wild-type controls. The electrical activity and neural responses of the circular layer are significantly different in aganglionic segments of ls/ls mutants.

scholarly journals Comparison of mechanical and electrical activity and interstitial cells of Cajal in urinary bladders from wild-type andW/Wvmice

2009 ◽  
Vol 156 (2) ◽  
pp. 273-283 ◽  
Author(s):  
KD McCloskey ◽  
UA Anderson ◽  
RA Davidson ◽  
YR Bayguinov ◽  
KM Sanders ◽  
...  
Keyword(s):  
Electrical Activity ◽  
Interstitial Cells Of Cajal ◽  
Interstitial Cells ◽  
Wild Type ◽  
Cells Of Cajal

scholarly journals Interstitial cells of Cajal generate spontaneous transient depolarizations in the rat gastric fundus

2009 ◽  
Vol 297 (4) ◽  
pp. G814-G824 ◽  
Author(s):  
Yoshihiko Kito ◽  
Kenton M. Sanders ◽  
Sean M. Ward ◽  
Hikaru Suzuki
Keyword(s):  
Electrical Activity ◽  
Interstitial Cells Of Cajal ◽  
Maximum Amplitude ◽  
Circular Muscle ◽  
Interstitial Cells ◽  
Gastric Fundus ◽  
Double Labeling ◽  
Membrane Hyperpolarization ◽  
Cells Of Cajal ◽  
Rat Gastric Fundus

Intracellular recordings were made from isolated circular muscle bundles of rat gastric fundus. The majority of cells generated an ongoing discharge of electrical activity that were ≤10 mV in amplitude (unitary potentials). A second pattern of electrical activity was recorded in less than 1% of all impalements. This electrical activity was characterized by high frequency, large amplitude spontaneous transient depolarizations (STDs) with a maximum rate of rise (d V/d tmax) of 0.5 V/s. Injection of the fluorescent dye propidium iodide into cells and double labeling with an antibody against the Kit receptor revealed that unitary potentials were recorded from circular smooth muscle cells (CSMC), whereas STDs were generated by intramuscular interstitial cells of Cajal (ICC-IM). Sustained injection periods (>15 min) resulted in the spread of dye between CSMC, between ICC-IM, and between CSMC and ICC-IM. Two types of STDs were observed, regularly occurring continuous STDs and irregular noisy bursting STDs. The amplitude of STDs varied between the two types of STDs. Single units summed to develop STDs with a maximum amplitude of 30 mV. Sodium nitroprusside (3 μM) induced membrane hyperpolarization and abolished unitary potentials generated by CSMC. In contrast, the amplitude of STDs generated by ICC-IM was increased with membrane hyperpolarization. Hyperpolarization induced by pinacidil (10 μM) also increased the amplitude of STDs and enhanced d V/d tmax. These observations indicate that STDs generated in ICC-IM spread passively to the adjacent CSMC to evoke the discharge of unitary potentials in the gastric fundus.

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