scholarly journals Characterization of Lysosomal Acid Lipase by Site-directed Mutagenesis and Heterologous Expression

1995 ◽  
Vol 270 (46) ◽  
pp. 27766-27772 ◽  
Author(s):  
Sulaiman Sheriff ◽  
Hong Du ◽  
Gregory A. Grabowski
Keyword(s):  
Heterologous Expression ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Acid Lipase ◽  
Lysosomal Acid ◽  
Lysosomal Acid Lipase

scholarly journals Characterization of lysosomal acid lipase mutations in the signal peptide and mature polypeptide region causing Wolman disease

2001 ◽  
Vol 42 (7) ◽  
pp. 1033-1040 ◽  
Author(s):  
Oliver Zschenker ◽  
Nikola Jung ◽  
Julia Rethmeier ◽  
Sabine Trautwein ◽  
Stefanie Hertel ◽  
...  
Keyword(s):  
Signal Peptide ◽  
Acid Lipase ◽  
Wolman Disease ◽  
Lysosomal Acid ◽  
Lysosomal Acid Lipase ◽  
Mature Polypeptide

Mouse lysosomal acid lipase: characterization of the gene and analysis of promoter activity

Gene ◽  
1998 ◽  
Vol 208 (2) ◽  
pp. 285-295 ◽  
Author(s):  
Hong Du ◽  
Ming Duanmu ◽  
Lillian Rosa Rosa
Keyword(s):  
Promoter Activity ◽  
Acid Lipase ◽  
Lysosomal Acid ◽  
Lysosomal Acid Lipase

scholarly journals A kinetic assay of total lipase activity for detecting lysosomal acid lipase deficiency (LAL‐D) and the molecular characterization of 18 LAL‐D patients from Russia

JIMD Reports ◽  
2019 ◽  
Vol 48 (1) ◽  
pp. 75-82
Author(s):  
Nikolay Mayanskiy ◽  
Ekaterina Brzhozovskaya ◽  
Alexander Pushkov ◽  
Tatiana Strokova ◽  
Nikolay Vlasov ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Lipase Activity ◽  
Acid Lipase ◽  
Kinetic Assay ◽  
Lysosomal Acid ◽  
Lysosomal Acid Lipase ◽  
Lysosomal Acid Lipase Deficiency

Heterologous expression of sarcoplasmic reticulum Ca2+-ATPase

1996 ◽  
Vol 16 (2) ◽  
pp. 107-113 ◽  
Author(s):  
Eduardo M. R. Reis ◽  
Carolyn W. Slayman ◽  
Sergio Verjovski-Almeida
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Recombinant Protein ◽  
Ligand Binding ◽  
Heterologous Expression ◽  
Eukaryotic Expression ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Expression Systems ◽  
Eukaryotic Expression Systems

In recent years, expression of rabbit sarcoplasmic reticulum (SR) Ca2+-ATPase in heterologous systems has been a widely used strategy to study altered enzymes generated by site-directed mutagenesis. Various eukaryotic expression systems have been tested, all of them yielding comparable amounts of recombinant protein. However, the relatively low yield of recombinant protein obtained so far suggests that novel purification techniques will be required to allow further characterization of this enzyme based on direct ligand-binding measurements.

Characterization of Lysosomal Acid Lipase Purified from Rabbit Liver1

1984 ◽  
Vol 96 (4) ◽  
pp. 1089-1101 ◽  
Author(s):  
Tsuneo IMANAKA ◽  
Kimiko AMANUMA-MUTO ◽  
Shoji OHKUMA ◽  
Tatsuya TAKANO
Keyword(s):  
Acid Lipase ◽  
Lysosomal Acid ◽  
Lysosomal Acid Lipase
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