scholarly journals Expression, Purification, and Metal Binding Properties of the N-terminal Domain from the Wilson Disease Putative Copper-transporting ATPase (ATP7B)

1997 ◽  
Vol 272 (52) ◽  
pp. 33279-33282 ◽  
Author(s):  
Michael DiDonato ◽  
Suree Narindrasorasak ◽  
John R. Forbes ◽  
Diane W. Cox ◽  
Bibudhendra Sarkar
Author(s):  
Martin Poenie ◽  
Akwasi Minta ◽  
Charles Vorndran

The use of fura-2 as an intracellular calcium indicator is complicated by problems of rapid dye leakage and intracellular compartmentalization which is due to a probenecid sensitive anion transporter. In addition there is increasing evidence for localized microdomains of high calcium signals which may not be faithfully reported by fura-2.We have developed a new family of fura-2 analogs aimed at addressing some of these problems. These new indicators are based on a modified bapta which can be readily derivatized to produce fura-2 analogs with a variety of new properties. The modifications do not affect the chromophore and have little impact on the spectral and metal binding properties of the indicator. One of these new derivatives known as FPE3 is a zwitterionic analog of fura-2 that can be loaded into cells as an acetoxymethyl ester and whose retention in cells is much improved. The improved retention of FPE3 is important for both cuvettebased measurements of cell suspensions and for calcium imaging.


2003 ◽  
Vol 5 (12) ◽  
pp. 2051-2054 ◽  
Author(s):  
Matthew A. Clark ◽  
Kathryn Duffy ◽  
Jyoti Tibrewala ◽  
Stephen J. Lippard

1983 ◽  
Vol 77 ◽  
pp. L63-L64 ◽  
Author(s):  
Bridget A. Sayer ◽  
Joseph P. Michael ◽  
Robert D. Hancock

Genes ◽  
2016 ◽  
Vol 7 (12) ◽  
pp. 106 ◽  
Author(s):  
Yu Pan ◽  
Yanglu Pan ◽  
Junpeng Zhai ◽  
Yan Xiong ◽  
Jinhua Li ◽  
...  

1994 ◽  
Vol 50 (3) ◽  
pp. 301-309 ◽  
Author(s):  
R.T. Pogue ◽  
W.X. Zhang ◽  
R.E. Wonkka ◽  
V. Majidi

1982 ◽  
Vol 66 ◽  
pp. 119-124 ◽  
Author(s):  
Shigeo Takeshima ◽  
Hiromu Sakurai

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