scholarly journals Molecular Cooperation between the Werner Syndrome Protein and Replication Protein A in Relation to Replication Fork Blockage

2010 ◽  
Vol 286 (5) ◽  
pp. 3497-3508 ◽  
Author(s):  
Amrita Machwe ◽  
Enerlyn Lozada ◽  
Marc S. Wold ◽  
Guo-Min Li ◽  
David K. Orren
2009 ◽  
Vol 284 (50) ◽  
pp. 34682-34691 ◽  
Author(s):  
Gregory Sowd ◽  
Hong Wang ◽  
Dalyir Pretto ◽  
Walter J. Chazin ◽  
Patricia L. Opresko

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Gyuho Yeom ◽  
Jinwoo Kim ◽  
Chin-Ju Park

Abstract Werner syndrome protein (WRN) and Fanconi anemia group J protein (FANCJ) are human DNA helicases that contribute to genome maintenance. They interact with replication protein A (RPA), and these interactions dramatically enhance the unwinding activities of both helicases. Even though the interplay between these helicases and RPA is particularly important in the chemoresistance pathway of cancer cells, the precise binding regions, interfaces, and properties have not yet been characterized. Here we present systematic NMR analyses and fluorescence polarization anisotropy assays of both helicase-RPA interactions for defining core binding regions and binding affinities. Our results showed that two acidic repeats of human WRN bind to RPA70N and RPA70A. For FANCJ, the acidic-rich sequence in the C-terminal domain is the binding region for RPA70N. Our results suggest that each helicase interaction has unique features, although they both fit an acidic peptide into a basic cleft for RPA binding. Our findings shed light on the protein interactions involved in overcoming the DNA-damaging agents employed in the treatment of cancer and thus potentially provide insight into enhancing the efficacy of cancer therapy.


FEBS Letters ◽  
2018 ◽  
Vol 592 (4) ◽  
pp. 547-558 ◽  
Author(s):  
Donguk Kang ◽  
Sungjin Lee ◽  
Kyoung‐Seok Ryu ◽  
Hae‐Kap Cheong ◽  
Eun‐Hee Kim ◽  
...  

2008 ◽  
Vol 283 (36) ◽  
pp. 24478-24483 ◽  
Author(s):  
Sarah A. Compton ◽  
Gökhan Tolun ◽  
Ashwini S. Kamath-Loeb ◽  
Lawrence A. Loeb ◽  
Jack D. Griffith

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