scholarly journals Differential Characteristics and Subcellular Localization of Two Starch-branching Enzyme Isoforms Encoded by a Single Gene inPhaseolus vulgaris L.

2002 ◽  
Vol 277 (19) ◽  
pp. 16538-16546 ◽  
Author(s):  
Shigeki Hamada ◽  
Hiroyuki Ito ◽  
Susumu Hiraga ◽  
Keisuke Inagaki ◽  
Kouichi Nozaki ◽  
...  
Keyword(s):  
Subcellular Localization ◽  
Single Gene ◽  
Branching Enzyme ◽  
Starch Branching Enzyme ◽  
Enzyme Isoforms

scholarly journals Differential Expression and Properties of Starch Branching Enzyme Isoforms in Developing Wheat Endosperm

1997 ◽  
Vol 113 (1) ◽  
pp. 201-208 ◽  
Author(s):  
M. K. Morell ◽  
A. Blennow ◽  
B. Kosar-Hashemi ◽  
M. S. Samuel
Keyword(s):  
Differential Expression ◽  
Branching Enzyme ◽  
Starch Branching Enzyme ◽  
Wheat Endosperm ◽  
Enzyme Isoforms

Two starch-branching-enzyme isoforms occur in different fractions of developing seeds of kidney bean

2001 ◽  
Vol 359 (1) ◽  
pp. 23-34 ◽  
Author(s):  
Shigeki HAMADA ◽  
Kouichi NOZAKI ◽  
Hiroyuki ITO ◽  
Yasushi YOSHIMOTO ◽  
Hironori YOSHIDA ◽  
...  
Keyword(s):  
Seed Development ◽  
Blot Analysis ◽  
Kidney Bean ◽  
Kinetic Properties ◽  
Branching Enzyme ◽  
Starch Branching Enzyme ◽  
Recombinant Enzymes ◽  
Significant Similarity ◽  
Developing Seeds ◽  
Enzyme Isoforms

The nature and enzymic properties of starch-branching enzyme (SBE) are two of the dominant factors influencing the fine structure of starch. To understand the role of this enzyme's activity in the formation of starch in kidney bean (Phaseolus vulgaris L.), a study was undertaken to identify the major SBE sequences expressed during seed development and to characterize the enzymic properties of the coded recombinant enzymes. Two SBE cDNA species (designated pvsbe2 and pvsbe1) that displayed significant similarity (more than 70%) to other family A and B SBEs respectively were isolated. Northern blot analysis revealed that pvsbe1 and pvsbe2 were differentially expressed during seed development. pvsbe2 showed maximum steady-state transcript levels at the mid-stage of seed maturation, whereas pvsbe1 reached peak levels at a later stage. Western blot analysis with antisera raised against both recombinant proteins (rPvSBE1 and rPvSBE2) showed that these two SBEs were located in different amyloplast fractions of developing seeds of kidney bean. PvSBE2 was present in the soluble fraction, whereas PvSBE1 was associated with the starch granule fraction. The differences in location suggest that these two SBE isoenzymes have different roles in amylopectin synthesis in kidney bean seeds. rPvSBE1 and rPvSBE2 were purified from Escherichia coli and their kinetic properties were determined. The affinity of rPvSBE2 for amylose (Km 1.27mg/ml) was lower than that of rPvSBE1 (0.46mg/ml). The activity of rPvSBE2 was stimulated more than 3-fold in the presence of 0.3M citrate, whereas rPvSBE1 activity was not affected. The implications of the enzymic properties and the distribution of SBEs and amylopectin structure are discussed.

scholarly journals Virus-induced Silencing SBEIIa and SSIIa, Alone or Together, Increased the Amylose and Resistant Starch Contents in Spring Wheat

2021 ◽  
Vol 25 (06) ◽  
pp. 1263-1271
Author(s):  
Zhaofeng Li
Keyword(s):  
Gene Silencing ◽  
Resistant Starch ◽  
Molecular Design ◽  
Single Gene ◽  
Starch Synthesis ◽  
Starch Synthase ◽  
Virus Induced Gene Silencing ◽  
Branching Enzyme ◽  
Starch Branching Enzyme ◽  
High Amylose

Foods rich in amylose and resistant starch (RS) have great potential to improve human health and lower the risk of noninfectious diseases. Common wheat (Triticum aestivumL.) is a major staple food crop with low RS content in the grains. The content of RS, preferentially derived from amylose, may be increased by suppressing amylopectin synthesis viasilencing the starch branching enzyme (SBE) II a or/and starch synthase (SS)IIa. In this study, SBEIIaand SSIIawere silenced separately and simultaneously using a barley stripe mosaic virus-virus-induced gene silencing (BSMV-VIGS) system. Compared with grains from control BSMV:00-inoculated spikes, grains from BSMV:SBEIIa-and BSMV:SSIIa-infected spikeshad fewer SBEIIa and SSIIa transcripts,together with increased amylose contents (18.62 and 24.48%, respectively) and RS contents (11.61 and 16.67%, respectively). Infection with BSMV:SBEIIa-SSIIa reduced SBEIIa and SSIIatranscript levels and increased the amylose and RS contents (32.02 and 22.33%, respectively). Thus, BSMV-VIGS is a useful tool for the rapid silencing of single or multiple starch synthase-related genes and BSMV shows great potential to study the functions of genes involved in starch biosynthesis or other processes/traits in developing grains.We showed that the SSIIagene plays an important role in the synthesis of amylose and RS and that the effects of simultaneously silencing SBEIIaand SSIIaon starch synthesis aregreater than those of single gene silencing. Our study lays the foundation for the molecular design-based breeding of high-amylose and high-RS wheat.© 2021 Friends Science Publishers

scholarly journals Two starch-branching-enzyme isoforms occur in different fractions of developing seeds of kidney bean

2001 ◽  
Vol 359 (1) ◽  
pp. 23 ◽  
Author(s):  
Shigeki HAMADA ◽  
Kouichi NOZAKI ◽  
Hiroyuki ITO ◽  
Yasushi YOSHIMOTO ◽  
Hironori YOSHIDA ◽  
...  
Keyword(s):  
Kidney Bean ◽  
Branching Enzyme ◽  
Starch Branching Enzyme ◽  
Developing Seeds ◽  
Enzyme Isoforms

Purification of two forms of starch branching enzyme (Q-enzyme) from developing rice endosperm

1992 ◽  
Vol 84 (3) ◽  
pp. 329-335 ◽  
Author(s):  
Yasunori Nakamura ◽  
Tetsuo Takeichi ◽  
Kentaro Kawaguchi ◽  
Hiroaki Yamanouchi
Keyword(s):  
Branching Enzyme ◽  
Starch Branching Enzyme ◽  
Rice Endosperm
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