scholarly journals Dominant-negative Inhibition of Pheromone Receptor Signaling by a Single Point Mutation in the G Protein α Subunit

2004 ◽  
Vol 279 (34) ◽  
pp. 35287-35297 ◽  
Author(s):  
Yuh-Lin Wu ◽  
Shelley B. Hooks ◽  
T. Kendall Harden ◽  
Henrik G. Dohlman
Keyword(s):  
G Protein ◽  
Point Mutation ◽  
Single Point ◽  
Dominant Negative ◽  
Single Point Mutation ◽  
Receptor Signaling ◽  
Α Subunit ◽  
Pheromone Receptor ◽  
G Protein Α Subunit

scholarly journals Selective Uncoupling of RGS Action by a Single Point Mutation in the G Protein α-Subunit

1998 ◽  
Vol 273 (10) ◽  
pp. 5780-5784 ◽  
Author(s):  
Paul R. DiBello ◽  
Tiffany Runyan Garrison ◽  
Donald M. Apanovitch ◽  
Ginger Hoffman ◽  
David J. Shuey ◽  
...  
Keyword(s):  
G Protein ◽  
Point Mutation ◽  
Single Point ◽  
Single Point Mutation ◽  
Α Subunit ◽  
G Protein Α Subunit

scholarly journals A single point mutation converts a proton-pumping rhodopsin into a red-shifted, turn-on fluorescent sensor for chloride

2021 ◽  
Author(s):  
Jasmine N. Tutol ◽  
Jessica Lee ◽  
Hsichuan Chi ◽  
Farah N. Faizuddin ◽  
Sameera S. Abeyrathna ◽  
...  
Keyword(s):  
Point Mutation ◽  
Fluorescent Sensor ◽  
Single Point ◽  
Proton Pumping ◽  
Single Point Mutation ◽  
Turn On ◽  
Gloeobacter Violaceus

By utilizing laboratory-guided evolution, we have converted the fluorescent proton-pumping rhodopsin GR from Gloeobacter violaceus into GR1, a red-shifted, turn-on fluorescent sensor for chloride.

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