scholarly journals p300/CBP-associated Factor Drives DEK into Interchromatin Granule Clusters

2005 ◽  
Vol 280 (36) ◽  
pp. 31760-31767 ◽  
Author(s):  
Joanne Cleary ◽  
Kajal V. Sitwala ◽  
Michael S. Khodadoust ◽  
Roland P. S. Kwok ◽  
Nirit Mor-Vaknin ◽  
...  
Keyword(s):  
Associated Factor ◽  
Interchromatin Granule Clusters ◽  
Interchromatin Granule

Organization of transcription and pre-mRNA splicing within the mammalian cell nucleus

1995 ◽  
Vol 53 ◽  
pp. 768-769
Author(s):  
D.L. Spector ◽  
S. Huang ◽  
S. Kaurin
Keyword(s):  
Rna Polymerase Ii ◽  
Cell Nucleus ◽  
Functional Organization ◽  
Mrna Splicing ◽  
Splicing Factors ◽  
Interchromatin Granule Clusters ◽  
Interchromatin Granule ◽  
Nuclear Regions ◽  
Relationship Of ◽  
Perichromatin Fibrils

We have been interested in the organization of RNA polymerase II transcription and pre-mRNA splicing within the cell nucleus. Several models have been proposed for the functional organization of RNA within the eukaryotic nucleus and for the relationship of this organization to the distribution of pre-mRNA splicing factors. One model suggests that RNAs which must be spliced are capable of recruiting splicing factors to the sites of transcription from storage and/or reassembly sites. When one examines the organization of splicing factors in the nucleus in comparison to the sites of chromatin it is clear that splicing factors are not localized in coincidence with heterochromatin (Fig. 1). Instead, they are distributed in a speckled pattern which is composed of both perichromatin fibrils and interchromatin granule clusters. The perichromatin fibrils are distributed on the periphery of heterochromatin and on the periphery of interchromatin granule clusters as well as being diffusely distributed throughout the nucleoplasm. These nuclear regions have been previously shown to represent initial sites of incorporation of 3H-uridine.

scholarly journals In vivo analysis of the stability and transport of nuclear poly(A)+ RNA.

1994 ◽  
Vol 126 (4) ◽  
pp. 877-899 ◽  
Author(s):  
S Huang ◽  
T J Deerinck ◽  
M H Ellisman ◽  
D L Spector
Keyword(s):  
Rna Polymerase Ii ◽  
Mammalian Cells ◽  
Stable Population ◽  
Nuclear Pore ◽  
Nuclear Pore Complexes ◽  
Nuclear Pores ◽  
Electron Microscopic ◽  
Interchromatin Granule Clusters ◽  
Interchromatin Granule ◽  
Pore Complexes

We have studied the distribution of poly(A)+ RNA in the mammalian cell nucleus and its transport through nuclear pores by fluorescence and electron microscopic in situ hybridization. Poly(A)+ RNA was detected in the nucleus as a speckled pattern which includes interchromatin granule clusters and perichromatin fibrils. When cells are fractionated by detergent and salt extraction as well as DNase I digestion, the majority of the nuclear poly(A)+ RNA was found to remain associated with the nonchromatin RNP-enriched fraction of the nucleus. After inhibition of RNA polymerase II transcription for 5-10 h, a stable population of poly(A)+ RNA remained in the nucleus and was reorganized into fewer and larger interchromatin granule clusters along with pre-mRNA splicing factors. This stable population of nuclear RNA may play an important role in nuclear function. Furthermore, we have observed that, in actively transcribing cells, the regions of poly(A)+ RNA which reached the nuclear pore complexes appeared as narrow concentrations of RNA suggesting a limited or directed pathway of movement. All of the observed nuclear pores contained poly(A)+ RNA staining suggesting that they are all capable of exporting RNA. In addition, we have directly visualized, for the first time in mammalian cells, the transport of poly(A)+ RNA through the nuclear pore complexes.

scholarly journals An Immunocytochemical Study of Interchromatin Granule Clusters in Early Mouse Embryos

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Irina Bogolyubova ◽  
Dmitry Bogolyubov
Keyword(s):  
Rna Polymerase Ii ◽  
De Novo ◽  
Gene Activation ◽  
Mouse Embryos ◽  
Molecular Composition ◽  
Interchromatin Granule Clusters ◽  
Interchromatin Granule ◽  
Zygotic Gene ◽  
Early Mouse ◽  
Nuclear Domains

Interchromatin granule clusters (IGCs) are universal nuclear domains. Their molecular composition and functions were studied in detail in somatic cells. Here, we studied IGCs in the nuclei of early mouse embryos during zygotic gene activation (ZGA). We found that the size of IGCs gradually increases during realization of ZGA events. Using immunocytochemical approaches, we showed that the molecular composition of IGCs is also modified in mouse embryos. The hyperphosphorylated form of RNA polymerase II and the transcription factor TFIID have been revealed in IGCs before the end of ZGA. Association of these factors with IGCs became more noticeable during ZGA realization. Our data suggest that IGCs in early mouse embryos have some functional peculiarities connected most probably with IGC formationde novo. We believe that IGCs in early mouse embryos not only are storage sites of splicing factors but also may be involved in mRNA metabolism and represent the multifunctional nuclear domains.

scholarly journals Cętki jądrowe jako modelowe ciałka jądrowe. Cętki jądrowe w układzie nerwowym

Kosmos ◽  
2020 ◽  
Vol 69 (1) ◽  
pp. 17-35
Author(s):  
Andrzej Szczepankiewicz
Keyword(s):  
Nuclear Bodies ◽  
Nuclear Speckles ◽  
Interchromatin Granule Clusters ◽  
Interchromatin Granule

Jądro komórkowe oprócz heterochromatyny czyli kompleksu DNA i białek histonowych, zawiera struktury zwane ciałkami jądrowymi (ang. nuclear bodies). Są to niewielkie, zazwyczaj koliste twory ?zawieszone? w nukleoplazmie, składające się z białek lub białek i niekodującego RNA. Znanych jest kilkanaście rodzajów takich struktur. Artykuł przedstawia obecny stan wiedzy na temat ciałek nazywanych w angielskiej literaturze nuclear speckles, czyli cętki jądrowe lub interchromatin granule clusters czyli skupiska ziaren interchromatynowych. Zaobserwowane po raz pierwszy przez Romana y Cajala w neuronach, obecne jednak we wszystkich typach komórek, struktury te uważane są za magazyny i miejsce modyfikacji czynników składania (splicingu) mRNA. Najnowsze badania, prezentowane w artykule pozwalają sądzić, że ich udział w metabolizmie RNA jest bardziej złożony. Świadczą o tym również doniesienia o roli cętek.

Nuclear organization of pre-mRNA splicing factors and their substrates

1994 ◽  
Vol 52 ◽  
pp. 18-19
Author(s):  
S. Huang ◽  
T. J. Deerinck ◽  
M. H. Ellisman ◽  
D. L. Spector
Keyword(s):  
Mammalian Cells ◽  
Large Body ◽  
Mrna Splicing ◽  
Electron Microscopic Level ◽  
Splicing Factors ◽  
Electron Microscopic ◽  
Speckled Pattern ◽  
Interchromatin Granule Clusters ◽  
Interchromatin Granule ◽  
Perichromatin Fibrils

Previous studies from our laboratory as well as other laboratories have shown that a variety of pre-mRNA splicing factors are localized to a subnuclear speckled domain when mammalian cells are immunolabeled with antibodies against these pre-mRNA splicing factors. At the electron microscopic level the speckled pattern is composed of both interchromatin granule clusters and perichromatin fibrils. A large body of evidence has accumulated from both our laboratory and other laboratories which has suggested that the perichromatin fibrils represent nascent transcripts and the interchromatin granule clusters represent storage and/or assembly sites for pre-mRNA splicing factors. The majority of substrates for these splicing factors are pre-mRNAs which contain a poly(A) tail of approximately 200-300 nucleotides. During the past year we have studied the distribution of poly(A)+ RNA in the mammalian cell nucleus and its transport through nuclear pores by fluorescence and electron microscopic in situ hybridization. Poly(A)+ RNA was detected in the nucleus as a speckled pattern which we have found to totally colocalize with pre-mRNA splicing factors at interchromatin granule clusters and perichromatin fibrils.

scholarly journals RCC1 and nuclear organization.

1997 ◽  
Vol 8 (6) ◽  
pp. 1143-1157 ◽  
Author(s):  
S Huang ◽  
A Mayeda ◽  
A R Krainer ◽  
D L Spector
Keyword(s):  
Microscopic Examination ◽  
Nuclear Organization ◽  
Electron Microscopic Examination ◽  
Splicing Factors ◽  
Restrictive Temperature ◽  
Permissive Temperature ◽  
Electron Microscopic ◽  
Double Labeling ◽  
Interchromatin Granule Clusters ◽  
Interchromatin Granule

We have examined the effect of RCC1 function on the nuclear organization of pre-mRNA splicing factors and poly(A)+ RNA in the tsBN2 cells, a RCC1 temperature-sensitive mutant cell line. We have found that at 4-6 h after shifting cells from the permissive temperature (32.5 degrees C) to the restrictive temperature (39.5 degrees C), both small nuclear ribonucleoprotein particles and a general splicing factor SC35 reorganized into 4-10 large round clusters in the nucleus, as compared with the typical speckled distribution seen in cells at the permissive temperature. In situ hybridization to poly(A)+ RNA resulted in a similar pattern. Examination by double labeling demonstrated that the redistribution of splicing factors coincides with that of poly(A)+ RNA. Such changes in the nuclear organization of splicing factors and poly(A)+ RNA were not the result of the temperature shift or of chromatin condensation. Cellular transcription was not significantly altered in these cells and extracts made from both the permissive and restrictive temperature were splicing competent. Electron microscopic examination demonstrated that the large clusters containing both splicing factors and poly(A)+ RNA were fused interchromatin granule clusters. In addition, small electron-dense dot-like structures measuring approximately 80 nm in diameter were also observed, most of which are accumulated in enlarged interchromatin granule clusters in the nucleoplasm of RCC1- cells. In spite of the significant changes observed in the nucleoplasm, relatively little alteration was observed in nucleolar structure by both light and electron microscopic examination. The above observations suggest that the RCC1 protein directly or indirectly regulates the organization of splicing components and poly(A)+ RNA in the cell nucleus and that RCC1 may play a role in nuclear organization.

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