The influence of soil microbes on plant diversity.

ABSTRACT Denitrification is an ecosystem process linked to ongoing climate change, because it releases nitrous oxide (N2O) into the atmosphere. To date, the literature covers mostly how aboveground (i.e. plant community structure) and belowground (i.e. plant-associated soil microbes) biota separately influence denitrification in isolation of each other. We here present a mesocosm experiment where we combine a manipulation of belowground biota (i.e. addition of Rhizophagus irregularis propagules to the indigenous mycorrhizal community) with a realized gradient in plant diversity. We used a seed mix containing plant species representative of mesophytic European grasslands and by stochastic differences in species establishment across the sixteen replicates per treatment level a spontaneously established gradient in plant diversity. We address mycorrhizal-induced and plant-diversity mediated changes on denitrification potential parameters and how these differ from the existing literature that studies them independently of each other. We show that unlike denitrification potential, N2O potential emissions do not change with mycorrhiza and depend instead on realized plant diversity. By linking mycorrhizal ecology to an N-cycling process, we present a comprehensive assessment of terrestrial denitrification dynamics when diverse plants co-occur.

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Plant diversity predicts beta but not alpha diversity of soil microbes across grasslands worldwide

Ecology Letters ◽

10.1111/ele.12381 ◽

2014 ◽

Vol 18 (1) ◽

pp. 85-95 ◽

Cited By ~ 302

Author(s):

Suzanne M. Prober ◽

Jonathan W. Leff ◽

Scott T. Bates ◽

Elizabeth T. Borer ◽

Jennifer Firn ◽

...

Keyword(s):

Plant Diversity ◽

Soil Microbes ◽

Alpha Diversity

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Rapid transfer of photosynthetic carbon through the plant-soil system in differently managed species-rich grasslands

Biogeosciences ◽

10.5194/bg-8-1131-2011 ◽

2011 ◽

Vol 8 (5) ◽

pp. 1131-1139 ◽

Cited By ~ 70

Author(s):

G. B. De Deyn ◽

H. Quirk ◽

S. Oakley ◽

N. Ostle ◽

R. D. Bardgett

Keyword(s):

Plant Species ◽

Plant Diversity ◽

Soil Microbes ◽

Soil Microbial Communities ◽

Short Term ◽

Soil System ◽

Plant Soil ◽

C Cycling ◽

C Assimilation ◽

Rapid Transfer

Abstract. Plant-soil interactions are central to short-term carbon (C) cycling through the rapid transfer of recently assimilated C from plant roots to soil biota. In grassland ecosystems, changes in C cycling are likely to be influenced by land use and management that changes vegetation and the associated soil microbial communities. Here we tested whether changes in grassland vegetation composition resulting from management for plant diversity influences short-term rates of C assimilation and transfer from plants to soil microbes. To do this, we used an in situ 13C-CO2 pulse-labelling approach to measure differential C uptake among different plant species and the transfer of the plant-derived 13C to key groups of soil microbiota across selected treatments of a long-term plant diversity grassland restoration experiment. Results showed that plant taxa differed markedly in the rate of 13C assimilation and concentration: uptake was greatest and 13C concentration declined fastest in Ranunculus repens, and assimilation was least and 13C signature remained longest in mosses. Incorporation of recent plant-derived 13C was maximal in all microbial phosopholipid fatty acid (PLFA) markers at 24 h after labelling. The greatest incorporation of 13C was in the PLFA 16:1ω5, a marker for arbuscular mycorrhizal fungi (AMF), while after 1 week most 13C was retained in the PLFA18:2ω6,9 which is indicative of assimilation of plant-derived 13C by saprophytic fungi. Our results of 13C assimilation and transfer within plant species and soil microbes were consistent across management treatments. Overall, our findings suggest that plant diversity restoration management may not directly affect the C assimilation or retention of C by individual plant taxa or groups of soil microbes, it can impact on the fate of recent C by changing their relative abundances in the plant-soil system. Moreover, across all treatments we found that plant-derived C is rapidly transferred specifically to AMF and decomposer fungi, indicating their consistent key role in the cycling of recent plant derived C.

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Faculty Opinions recommendation of Soil microbes drive the classic plant diversity-productivity pattern.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.13407990.14778095 ◽

2011 ◽

Author(s):

Helene Muller-Landau

Keyword(s):

Plant Diversity ◽

Soil Microbes ◽

Productivity Pattern

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Rapid transfer of photosynthetic carbon through the plant-soil system in differently managed grasslands

Biogeosciences Discussions ◽

10.5194/bgd-8-921-2011 ◽

2011 ◽

Vol 8 (1) ◽

pp. 921-940 ◽

Cited By ~ 1

Author(s):

G. B. De Deyn ◽

H. Quirk ◽

S. Oakley ◽

N. Ostle ◽

R. D. Bardgett

Keyword(s):

Plant Species ◽

Plant Diversity ◽

Soil Microbes ◽

Soil Microbial Communities ◽

Short Term ◽

Soil Microbial ◽

Plant Soil ◽

C Cycling ◽

Rapid Transfer

Abstract. Plant-soil interactions are central to short-term carbon (C) cycling through the rapid transfer of recently assimilated C from plant roots to soil biota. In grassland ecosystems, changes in C cycling are likely to be influenced by land use and management that changes vegetation and the associated soil microbial communities. Here we tested whether changes in grassland vegetation composition resulting from management for plant diversity influences short-term rates of C assimilation, retention and transfer from plants to soil microbes. To do this, we used an in situ 13C-CO2 pulse-labeling approach to measure differential C uptake among different plant species and the transfer of the plant-derived 13C to key groups of soil microbiota across selected treatments of a long-term plant diversity grassland restoration experiment. Results showed that plant taxa differed markedly in the rate of 13C assimilation and retention: uptake was greatest and retention lowest in Ranunculus repens, and assimilation was least and retained longest in mosses. Incorporation of recent plant-derived 13C was maximal in all microbial phosopholipid fatty acid (PLFA) markers at 24 h after labeling. The greatest incorporation of 13C was in the PLFA 16:1ω5, a marker for arbuscular mycorrhizal fungi (AMF), while after one week most 13C was retained in the PLFA 18:2ω6,9 which is indicative of assimilation of plant-derived 13C by saprophytic fungi. Our results of 13C assimilation, transfer and retention within plant species and soil microbes were consistent across management treatments. Overall, our findings suggest that changes in vegetation and soil microbial composition resulting from differences in long-term grassland management will affect short-term cycling of photosynthetic C, but that restoration management does not alter the short-term C uptake and transfer within plant species and within key groups of soil microbes. Moreover, across all treatments we found that plant-derived C is rapidly transferred specifically to AMF and decomposer fungi, indicating their consistent key role in the cycling of recent plant derived C.

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Plant diversity and species richness of Ljubljana marsh grasslands under the influence of different cutting and fertilizing regimes

Acta Agronomica Hungarica ◽

10.1556/aagr.57.2009.2.11 ◽

2009 ◽

Vol 57 (2) ◽

pp. 197-203 ◽

Cited By ~ 2

Author(s):

T. Sinkovč

Keyword(s):

Species Richness ◽

Plant Diversity ◽

Grassland Management ◽

Botanical Composition ◽

Significant Negative Effect ◽

Negative Effect ◽

Cutting Regimes ◽

N Rates ◽

Exploitation Intensity ◽

Plot Design

The botanical composition of grasslands determines the agronomic and natural values of swards. Good grassland management usually improves herbage value, but on the other hand it frequently decreases the plant diversity and species richness in the swards. In 1999 a field trial in a split-plot design with four replicates was therefore established on the Arrhenatherion type of vegetation in Ljubljana marsh meadows in order to investigate this relationship. Cutting regimes (2 cuts — with normal and delayed first cut, 3 cuts and 4 cuts per year) were allocated to the main plots and fertiliser treatments (zero fertiliser — control, PK and NPK with 2 or 3 N rates) were allocated to the sub-plots. The results at the 1 st cutting in the 5 th trial year were as follows: Fertilising either with PK or NPK had no significant negative effect on plant diversity in any of the cutting regimes. In most treatments the plant number even increased slightly compared to the control. On average, 20 species were listed on both unfertilised and fertilised swards. At this low to moderate level of exploitation intensity, the increased number of cuts had no significant negative effect on plant diversity either (19 species at 2 cuts vs. 20 species at 3 or 4 cuts). PK fertilisation increased the proportion of legumes in the herbage in the case of 2 or 3 cuts. The proportion of grasses in the herbage increased in all the fertilisation treatments with an increased numbers of cuts. Fertiliser treatment considerably reduced the proportion of marsh horsetail ( Equisetum palustre ) in the herbage of the meadows. This effect was even more pronounced at higher cut numbers. The proportion of Equisetum palustre in the herbage was the highest in the unfertilised sward with 2 cuts (26.4 %) and the lowest in the NPK-fertilised sward with 4 cuts (1.4%).

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