Stable carbon isotope fractionation of fatty acid in sea cucumber (Apostichopus japonicus): insights from an experimental study

2021 ◽  
pp. 1-13
Author(s):  
Xufeng Zhang ◽  
Yu Liu
Keyword(s):  
Experimental Study ◽  
Fatty Acid ◽  
Carbon Isotope ◽  
Sea Cucumber ◽  
Isotope Fractionation ◽  
Apostichopus Japonicus ◽  
Stable Carbon Isotope ◽  
Stable Carbon ◽  
Carbon Isotope Fractionation ◽  
Stable Carbon Isotope Fractionation

scholarly journals Stable Carbon Isotope Fractionation by Methylotrophic Methanogenic Archaea

2012 ◽  
Vol 78 (21) ◽  
pp. 7596-7602 ◽  
Author(s):  
Jörn Penger ◽  
Ralf Conrad ◽  
Martin Blaser
Keyword(s):  
Carbon Isotope ◽  
Isotope Fractionation ◽  
Stable Carbon Isotope ◽  
Methanogenic Archaea ◽  
Methanosarcina Barkeri ◽  
Stable Carbon ◽  
Methyl Fluoride ◽  
Content Type ◽  
Carbon Isotope Fractionation ◽  
Stable Carbon Isotope Fractionation

ABSTRACTIn natural environments methane is usually produced by aceticlastic and hydrogenotrophic methanogenic archaea. However, some methanogens can use C1compounds such as methanol as the substrate. To determine the contributions of individual substrates to methane production, the stable-isotope values of the substrates and the released methane are often used. Additional information can be obtained by using selective inhibitors (e.g., methyl fluoride, a selective inhibitor of acetoclastic methanogenesis). We studied stable carbon isotope fractionation during the conversion of methanol to methane inMethanosarcina acetivorans,Methanosarcina barkeri, andMethanolobus zinderiand generally found large fractionation factors (−83‰ to −72‰). We further tested whether methyl fluoride impairs methylotrophic methanogenesis. Our experiments showed that even though a slight inhibition occurred, the carbon isotope fractionation was not affected. Therefore, the production of isotopically light methane observed in the presence of methyl fluoride may be due to the strong fractionation by methylotrophic methanogens and not only by hydrogenotrophic methanogens as previously assumed.

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