Zonal Density Gradient Electrophoresis of Serum Albumin and Bacteriophages M13, φ X174, and MS2. Electrophoretic Mobility Measurement

1986 ◽  
Vol 21 (2) ◽  
pp. 187-207 ◽  
Author(s):  
C. J. P. Mullon ◽  
N. S. Mason ◽  
R. E. Sparks
1980 ◽  
Vol 51 (2) ◽  
pp. 238-249 ◽  
Author(s):  
Chris D. Platsoucas ◽  
Robert A. Good ◽  
Sudhir Gupta

1980 ◽  
Vol 63 (3) ◽  
pp. 258-265 ◽  
Author(s):  
C.D. Platsoucas ◽  
S.G. Robbins ◽  
N. Catsimpoolas

1988 ◽  
Vol 9 (10) ◽  
pp. 647-649 ◽  
Author(s):  
Lindsay D. Plank ◽  
M. Elaine Kunze ◽  
Richard A. Gaines ◽  
Paul Todd

1976 ◽  
Vol 25 (2) ◽  
pp. 317-321 ◽  
Author(s):  
N. Catsimpoolas ◽  
A.L. Griffith ◽  
E.M. Skrabut ◽  
C.D. Platsoucas ◽  
C.R. Valeri

1996 ◽  
Vol 17 (1) ◽  
pp. 173-178 ◽  
Author(s):  
Abraham Tulp ◽  
Desirée Verwoerd ◽  
Mar Fernandez-Borja ◽  
Jacques Neefjes ◽  
Augustinus A. M. Hart

1963 ◽  
Vol 16 (3) ◽  
pp. 471-481 ◽  
Author(s):  
Alexandra von der Decken

Ribonucleoprotein particles were prepared by treatment of rat liver microsomes with detergents and high concentrations of KCl. They were active in incorporating 14C amino acids into protein when incubated with cell sap together with ATP, GTP, and a system to regenerate the triphosphates. The albumin of the incubation mixture, soluble at 105,000 g, and that of the fraction released by ultrasonication of the particles were studied by immunoelectrophoresis in agar gel. When the ribonucleoprotein particles were incubated with cell sap the immunological precipitation lines formed with antiserum to rat serum albumin were highly radioactive as tested by autoradiography. After zone electrophoresis on cellulose acetate, two immunologically reactive albumins were obtained which differed in their electrophoretic mobility from rat serum albumin. Labeled albumin, when purified on DEAE-cellulose columns, retained its radioactivity as tested by autoradiography following immunoelectrophoresis. On cellulose acetate this purified albumin showed an electrophoretic mobility higher than that of rat serum albumin.


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