Separation of Proteic Primary Amino Acids under Several Reversed‐Phase Liquid Chromatographic Conditions

2006 ◽  
Vol 29 (17) ◽  
pp. 2521-2536 ◽  
Author(s):  
V. Concha‐Herrera ◽  
J. R. Torres‐Lapasió ◽  
G. Vivó‐Truyols ◽  
M. C. García‐Álvarez‐Coque
Keyword(s):  
Amino Acids ◽  
Reversed Phase ◽  
Primary Amino ◽  
Phase Liquid ◽  
Liquid Chromatographic

Reversed-Phase Liquid Chromatographic Determination of Hypoglycin A (HG-A) in Canned Ackee Fruit Samples

1994 ◽  
Vol 77 (5) ◽  
pp. 1175-1179 ◽  
Author(s):  
Ghulam Sarwar ◽  
Herbert G Botting
Keyword(s):  
Amino Acids ◽  
Chromatographic Determination ◽  
Reversed Phase ◽  
Precolumn Derivatization ◽  
Edible Portion ◽  
Fruit Samples ◽  
Liquid Chromatographic Determination ◽  
Phase Liquid ◽  
Liquid Chromatographic

Abstract A reversed-phase liquid chromatographic (LC) method involving precolumn derivatization with phenylisothiocyanate (PITC) was developed for determining levels of hypoglycin A (HG-A) in canned ackee fruit samples. HG-A was extracted by homogenizing the drained fruit in 80% ethanol. By using a Waters Pico-Tag amino acid analysis 15-cm-long column (which is also used for analyzing protein hydrolysates and biological samples) and an LC system, the baseline separation of HG-A from other amino acids was completed in about 6 min. The total time for analysis and equilibration was 16 min. HG-A levels in the edible portion of fruit in 18 cans varied from 18.27 to 87.50 mg HG-A/can. Recoveries of added standard HG-A averaged 101%. To our knowledge, this is the first report of the use of this method to determine HG-A in ackee fruit.

scholarly journals Reversed-Phase Liquid Chromatographic Profile of Free Amino Acids in Strawberry-tree (Arbutus unedo L.) Honey

2009 ◽  
Vol 92 (4) ◽  
pp. S1145-S1156 ◽  
Author(s):  
Nadia Spano ◽  
Irene Piras ◽  
Marco Ciulu ◽  
Ignazio Floris ◽  
Angelo Panzanelli ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Free Amino Acid ◽  
Free Amino Acids ◽  
Free Amino ◽  
Reversed Phase ◽  
Arbutus Unedo ◽  
Strawberry Tree ◽  
Phase Liquid ◽  
Liquid Chromatographic

Abstract The typical profile of the free amino acids contained in strawberry-tree (Arbutus unedo L.) honey is reported for the first time. An optimized reversed-phase liquid chromatographic (RP-LC) method with phenyl isothiocyanate precolumn derivatization was used. Fourteen free amino acids were identified and quantified in 16 analytical samples. Proline (65.63) was found to be the most abundant free amino acid, followed by glutamic acid (6.49), arginine (5.21), alanine (5.17), and phenylalanine (4.97). The total free amino acid content of strawberry-tree honey (average value, 436 mg/kg) was found to be low in comparison to amounts cited in the literature concerning unifloral honeys. The analytical method was optimized and fully validated in terms of detection and quantitation limits, precision (by testing repeatability and reproducibility), linearity, and bias (by means of recovery tests). The acceptability of the validation protocol results was verified using Horwitz's mathematical model and AOAC guidelines.

Reversed-phase liquid chromatographic resolution of underivatized d,l-amino acids using chiral eluents

1982 ◽  
Vol 239 ◽  
pp. 671-675 ◽  
Author(s):  
Noriyuki Nimura ◽  
Atsuko Toyama ◽  
Yoko Kasahara ◽  
Toshio Kinoshita
Keyword(s):  
Amino Acids ◽  
Reversed Phase ◽  
Phase Liquid ◽  
Chromatographic Resolution ◽  
Liquid Chromatographic

Determination of Amino Acids in Food and Feed by Derivatization with 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate and Reversed-Phase Liquid Chromatographic Separation

1995 ◽  
Vol 78 (3) ◽  
pp. 736-743 ◽  
Author(s):  
Hong Ji Liu ◽  
Bi Ying Chang ◽  
Hui Wen Yan ◽  
Feng Hua Yu ◽  
Xing Xiang Liu
Keyword(s):  
Amino Acids ◽  
Reversed Phase ◽  
Exchange Chromatography ◽  
Cysteic Acid ◽  
Precolumn Derivatization ◽  
Methionine Sulfone ◽  
Relative Standard ◽  
Food And Feed ◽  
Phase Liquid ◽  
Liquid Chromatographic

Abstract A study of a new amino acid analysis method using 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate as a precolumn derivatization reagent for the analysis of food and feed is described. All amino acids, including methionine sulfone and cysteic acid, were well separated on a liquid chromatographic system using the optimized chromatographic conditions. Salts in food and feed interfered very slightly with the derivatization yields of all amino acids. Several typical agricultural products and animal feeds, including 2 AOAC test samples, were analyzed with the method. The results agreed well with the data generated by using the classical postcolumn method with ion-exchange chromatography. The average relative standard deviations for corn and broiler starter feed were 0.74 and 0.70%, respectively. Good recoveries of all amino acids were demonstrated (average, 101%), even for a sample with a very complex matrix.

scholarly journals Reversed-Phase Liquid Chromatographic Profile of Free Amino Acids in Strawberry-tree (Arbutus unedo L.) Honey

2009 ◽  
Vol 92 (4) ◽  
pp. S73-S84 ◽  
Author(s):  
Nadia Spano ◽  
Irene Piras ◽  
Marco Ciulu ◽  
Ignazio Floris ◽  
Angelo Panzanelli ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Free Amino Acid ◽  
Free Amino Acids ◽  
Free Amino ◽  
Reversed Phase ◽  
Arbutus Unedo ◽  
Strawberry Tree ◽  
Phase Liquid ◽  
Liquid Chromatographic

Abstract The typical profile of the free amino acids contained in strawberry-tree (Arbutus unedo L.) honey is reported for the first time. An optimized reversed-phase liquid chromatographic (RP-LC) method with phenyl isothiocyanate precolumn derivatization was used. Fourteen free amino acids were identified and quantified in 16 analytical samples. Proline (65.63) was found to be the most abundant free amino acid, followed by glutamic acid (6.49), arginine (5.21), alanine (5.17), and phenylalanine (4.97). The total free amino acid content of strawberry-tree honey (average value, 436 mg/kg) was found to be low in comparison to amounts cited in the literature concerning unifloral honeys. The analytical method was optimized and fully validated in terms of detection and quantitation limits, precision (by testing repeatability and reproducibility), linearity, and bias (by means of recovery tests). The acceptability of the validation protocol results was verified using Horwitz's mathematical model and AOAC guidelines.

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