scholarly journals Absence of Telomerase Activity in Malignant Bone Tumors and Soft-Tissue Sarcomas

Sarcoma ◽  
2002 ◽  
Vol 6 (1) ◽  
pp. 43-46 ◽  
Author(s):  
Nina K. Lauer ◽  
Sandra M. Maier ◽  
Martin Oberringer ◽  
Michael Schulte ◽  
Wolf Mutschler ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Bone Tumors ◽  
Soft Tissue Sarcomas ◽  
Telomerase Activity ◽  
Telomeric Repeat ◽  
Malignant Bone Tumors ◽  
Trap Assay ◽  
Cell Immortalization ◽  
Amplification Assay ◽  
Subordinate Role

Purpose:Telomerase activity appears to play a crucial role in the development of many tumors. More than 80% of all malignant human tumors show an increased telomerase activity. However, conflicting results have been reported about telomerase activity in sarcomas. The aim of the study was to obtain more information about telomerase activity in sarcomas based on a large number of cases.Methods:Telomerase activity was measured in 69 different tumor samples (33 malignant bone tumors and 36 soft tissue sarcomas). Tumor samples were obtained intraoperatively and frozen immediately in liquid nitrogen. Telomerase activity was detected by the telomeric repeat amplification assay (TRAP-assay).Results:Only 7% of the samples showed telomerase activity. No correlation between staging and telomerase activity could be observed.Discussion:The fact that only five out of 69 examined tumor samples showed a telomerase activity provides experimental evidence that in sarcomas the reactivation of telomerase may play a subordinate role. Our results suggest that alternative mechanisms for cell immortalization, yet to be determined, seem to be involved in the development and/or maintenance of soft-tissue sarcomas and malignant bone tumors.

Lymphographic Evaluation in Bone and Soft Tissue Sarcomas

1977 ◽  
Vol 63 (3) ◽  
pp. 283-288 ◽  
Author(s):  
Renato Musumeci ◽  
Achille Bombarda ◽  
Ignazio Cataldo ◽  
Franco Fontana ◽  
Raffaele Petrillo ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Lymph Nodes ◽  
Bone Tumors ◽  
Ewing's Sarcoma ◽  
Soft Tissue Sarcomas ◽  
Diagnostic Workup ◽  
Reticulum Cell ◽  
Reticulum Cell Sarcoma ◽  
Malignant Bone Tumors ◽  
Cell Sarcoma

A group of 79 patients with soft tissue sarcomas and 46 with primary malignant bone tumors underwent lymphangiography as part of the initial diagnostic workup. In the group of soft tissue sarcomas, the overall incidence of metastases was 22 of 79 (28%). According to the site of origin, the highest incidence was found in tumors originating from the buttocks (57%), followed by those from the inferior limbs (31%). Considering the histology, the incidence ranges from 50 % in anaplastic sarcoma, to 43 % in rhabdomyosarcoma, to 23 % for liposarcoma and fibrosarcoma. The incidence of lymphographycally proven metastases in bone tumors was 8 of 46 (17 %), with 1 out of 4 in reticulum cell sarcoma, 1/1 in chordoma, and 22 % in Ewing's sarcoma. A radiographic/histologic correlation on the lymph nodes was obtained in 19/79 (24 %) soft tissue sarcomas and in 4/46 (9 %) bone tumors. In the 12 radiographically negative and 11 radiographically positive cases, this correlation was always correct. The results of this study suggest a larger use of lymphography in these tumors.

Scintigraphy with 99m Tc-Phosphonate and 67Gallium in Patients Suspected of Primary Bone Tumors

1982 ◽  
Vol 21 (04) ◽  
pp. 136-139 ◽  
Author(s):  
C.-J. Edeling
Keyword(s):  
Soft Tissue ◽  
Bone Scintigraphy ◽  
Primary Tumor ◽  
Bone Tumors ◽  
Whole Body ◽  
Skeletal Metastases ◽  
Malignant Bone Tumors ◽  
67Ga Scintigraphy ◽  
Primary Bone Tumors ◽  
Primary Bone

Whole-body scintigraphy with both 99mTc-phosphonate and 67Ga was performed on 92 patients suspected of primary bone tumors. In 46 patients with primary malignant bone tumors, scintigraphy with 99mTc-phosphonate disclosed the primary tumor in 44 cases and skeletal metastases in 11, and 67Ga scintigraphy detected the primary tumor in 43 cases, skeletal metastases in 6 cases and soft-tissue metastases in 8 cases. In 25 patients with secondary malignant bone tumors, bone scintigraphy visualized a single lesion in 10 cases and several lesions in 15 cases, and 67Ga scintigraphy detected the primary tumor in 17 cases, skeletal metastases in 17 cases and soft-tissue metastases in 9 cases. In 21 patients with benign bone disease positive uptake of 99mTc-phosphonate was recognized in 19 cases and uptake of 67Ga in 17 cases. It is concluded that bone scintigraphy should be used in patients suspected of primary bone tumors. If malignancy is suspected, 67Ga scintigraphy should be performed in addition.

Bone Tumors and Soft Tissue Sarcomas

2014 ◽  
pp. 536-536
Author(s):  
Ajay Puri ◽  
Nirmala Jambhekar
Keyword(s):  
Soft Tissue ◽  
Bone Tumors ◽  
Soft Tissue Sarcomas

scholarly journals Rapid, quantitative nonisotopic assay for telomerase activity in human tumors

1998 ◽  
Vol 44 (10) ◽  
pp. 2133-2138 ◽  
Author(s):  
Stefania Gelmini ◽  
Anna Caldini ◽  
Lucia Becherini ◽  
Sergio Capaccioli ◽  
Mario Pazzagli ◽  
...  
Keyword(s):  
Pcr Amplification ◽  
Telomerase Activity ◽  
Quantitative Information ◽  
Cellular Protein ◽  
Large Variability ◽  
Trap Assay ◽  
Mean Values ◽  
Double Stranded Dna ◽  
Amplification Protocol ◽  
Cell Immortalization

Abstract Telomerase is a ribonucleoprotein enzyme that adds TTAGGG repeats onto human telomeres, preventing their shortening. The activation of this enzyme is an important step in cell immortalization and carcinogenesis and seems to represent a new and promising marker in cancer diagnosis and management. Telomerase activity is usually detected in cellular protein extract by the telomeric repeat amplification protocol (TRAP) assay, which can provide only a qualitative (presence/absence) evaluation. Here we present a modification of this method that can provide quantitative information without requiring time-consuming post-PCR procedures such as gel electrophoresis with radioactive materials and autoradiography. The detection and measurement of telomerase activity is performed by evaluating the amount of double-stranded DNA generated in the telomerase reaction and PCR amplification, with the use of the sensitive DNA fluorescent dye PicoGreen®. In a subset of tumors, the presence of telomerase activity was confirmed by the conventional TRAP assay. By this method we evaluated telomerase activity in unselected groups of breast (n = 15), ovarian (n = 12), endometrial (n = 12), gastric (n = 20), and renal (n = 12) carcinomas, in meningiomas (n = 8), and in pheochromocitomas (n = 10). The results indicate substantial differences of telomerase activity among cancer groups; however, a large variability among patients of the same group is observed. Kidney, ovarian, and breast carcinomas showed the highest mean values (31.8 ± 28.9, 29.2 ± 26.7, and 35.3 ± 15.9 ng DNA/μg protein, respectively, mean ± SD), whereas gastric and endometrial cancers had a lower activity (17.2 ± 8.8 and 13.5 ± 7.9 ng DNA/μg protein, respectively). Very low or no detectable telomerase activity was found in meningiomas (with the exception of one malignant atypical variant) and pheochromocitomas (9.7 ± 12.9 and 2.8 ± 2.1 ng DNA/μg protein, respectively). In conclusion, our method seems to be an accurate and reasonable procedure for measuring telomerase activity in human cancers.

scholarly journals Real-Time Quantitative Telomeric Repeat Amplification Protocol Assay for the Detection of Telomerase Activity2

2001 ◽  
Vol 47 (3) ◽  
pp. 519-524 ◽  
Author(s):  
Mi Hou ◽  
Dawei Xu ◽  
Magnus Björkholm ◽  
Astrid Gruber
Keyword(s):  
Real Time ◽  
Half Life ◽  
Pcr Amplification ◽  
Human Tumor ◽  
Telomerase Activity ◽  
Quantitative Information ◽  
Telomeric Repeat ◽  
Telomeric Repeat Amplification Protocol ◽  
Trap Assay ◽  
Amplification Protocol

Abstract Background: Telomerase is a ribonucleoprotein enzyme associated with immortalization and transformation of human cells. The telomeric repeat amplification protocol (TRAP) is widely used for the detection of telomerase activity. The TRAP method, although highly sensitive and specific because it includes PCR amplification, is laborious and does not provide precise quantitative information. Methods: We developed a real-time quantitative TRAP (RTQ-TRAP) system by combining a real-time PCR technique with the conventional TRAP method. Telomerase activity in human tumor cell lines and in 13 lymphoma samples was measured using the RTQ-TRAP assay, and the results obtained from the samples using the RTQ-TRAP method were compared with the conventional TRAP method. Results: The RTQ-TRAP method was both accurate and reproducible in measuring telomerase activity in a dilution series of protein extracts from HL60 cells. Telomerase activity in 13 lymphoma samples, as determined by the RTQ-TRAP method, was ninefold lower than that measured by the conventional TRAP method. The half-life of telomerase activity in human tumor cells, as determined using RTQ-TRAP, was much shorter than the half-life reported previously. Conclusions: Our results suggest that the conventional TRAP assay frequently overestimates telomerase activity in tumor samples. The RTQ-TRAP method is thus a useful tool to rapidly and precisely quantify telomerase activity.

Prognostic significance of telomerase activity in soft tissue sarcomas.

1999 ◽  
Author(s):  
R Schneider-Stock ◽  
J Rys ◽  
V Jaeger ◽  
A Niezabitowski ◽  
A Kruczak ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Prognostic Significance ◽  
Soft Tissue Sarcomas ◽  
Telomerase Activity

Lymphography in Bone and Soft Tissue Sarcomas. Experiences from Three Institutions

1980 ◽  
Vol 66 (6) ◽  
pp. 721-728 ◽  
Author(s):  
Kaj Tallroth ◽  
Francisco Makai ◽  
Renato Musumeci
Keyword(s):  
Lymph Node ◽  
Soft Tissue ◽  
Diagnostic Accuracy ◽  
Bone Tumors ◽  
Soft Tissue Sarcomas ◽  
Hematogenous Spread ◽  
Primary Bone Tumor ◽  
Bone Sarcomas ◽  
Neurogenic Sarcoma ◽  
Primary Bone

The case material was collected from 3 Institutions with a total of 411 patients: 217 with primary bone tumor and 224 with soft tissue sarcomas. In the majority of patients lymphography was performed during the initial diagnostic workup. The lymphograms were interpreted as negative or positive for metastases. In bone tumors, the incidence of metastases was 21 %, ranging from 28 % for osteosarcoma to 18 % for Ewing's sarcoma and 13 % for chondrosarcoma. In tumors of the soft tissue, the frequency was somewhat higher (28%), with special regard to rhabdomyosarcoma (53%), anaplastic sarcoma (67 %), neurogenic sarcoma (42%) and synovial sarcoma (35%). In the group of bone sarcomas, primary hematogenous spread was 3 times more frequent than lymphogenous spread, while in soft tissue sarcomas, with a higher incidence of lymphatic spread, this finding was inverted. In the more consistent tumor groups, the occurrence of lymphatic metastases indicated a significant worsening of the prognosis. In 96 patients, lymph node biopsies were performed and the radiologic histologic correlation gave evidence of a 91.7 % overall diagnostic accuracy.

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