Antioxidant and cytoprotective effects of avocado oil and extract (Persea americana Mill) against rotenone using monkey kidney epithelial cells (Vero)

2021 ◽  
pp. 1-16
Author(s):  
Nelzi Ferreira Queiroz Junior ◽  
Jovani Antônio Steffani ◽  
Larissa Machado ◽  
Pâmela Jéssyca Hoss Longhi ◽  
Marco Aurélio Echart Montano ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Persea Americana ◽  
Monkey Kidney ◽  
Avocado Oil ◽  
Kidney Epithelial Cells

scholarly journals CYTOLOGICAL ABERRATIONS IN CULTURES OF "NORMAL" MONKEY KIDNEY EPITHELIAL CELLS

1958 ◽  
Vol 107 (4) ◽  
pp. 599-606 ◽  
Author(s):  
Ruth Kleinfeld ◽  
Joseph L. Melnick
Keyword(s):  
Epithelial Cells ◽  
Mitotic Activity ◽  
Primary Cultures ◽  
Mitotic Rate ◽  
Monkey Kidney ◽  
Mitotic Abnormalities ◽  
Normal Monkey ◽  
Dividing Cells ◽  
Chromosome Fragments ◽  
Kidney Epithelial Cells

A cytological study was made of short term primary cultures of monkey kidney epithelial cells grown in serum-containing (M-H) and synthetic (SM-2) media. The mitotic activity of the cultures reached a peak 3 to 5 days after seeding, and rapidly declined if the medium was not changed. With replenishment at 48 hour intervals a high mitotic rate was maintained through the 8th day. Cultures subjected to a glycine- and glucose-deficient medium showed a sharp decline in mitotic activity within 24 hours. When such cultures were replenished a burst of mitotic activity occurred 20 to 30 hours later. Mitotic aberrations such as multipolar spindles, anomalous spindles with scattered chromosomes, lagging or precocious movement of chromosomes, chromosome fragments, and chromosome bridges were found in all cultures ranging from 16 to 40 per cent of the dividing cells. Non-mitotic aberrations such as giant nuclei, multinucleate cells, nuclear fragmentation, micronuclei, nuclear inclusions, and phagocytosis were found in approximately 3 to 7 per cent of the non-dividing cell population. The frequency and types of mitotic abnormalities were generally independent of the medium used and the mitotic index of the cultures.

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