Development of High-Performance Liquid Chromatographic Method for Determination of Brotizolam in Human Plasma. A Clinical Pharmacokinetic Study

2003 ◽  
Vol 36 (14) ◽  
pp. 3025-3034 ◽  
Author(s):  
T. Osanai ◽  
T. Ohkubo
Keyword(s):  
Human Plasma ◽  
Clinical Pharmacokinetic ◽  
Pharmacokinetic Study ◽  
High Performance ◽  
Chromatographic Method ◽  
High Performance Liquid Chromatographic ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Fluoroimmunoassays for procainamide and N-acetylprocainamide compared with a liquid-chromatographic method.

1984 ◽  
Vol 30 (5) ◽  
pp. 768-773 ◽  
Author(s):  
J J MacKichan ◽  
J D Coyle ◽  
B J Shields ◽  
H Boudoulas ◽  
J J Lima
Keyword(s):  
Excellent Agreement ◽  
Pharmacokinetic Study ◽  
Active Metabolite ◽  
High Performance ◽  
Chromatographic Method ◽  
High Performance Liquid Chromatographic ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Abstract We measured procainamide and its active metabolite, N-acetylprocainamide (NAPA), in 80 sera from 37 patients by a new fluorimmunoassay procedure and an established "high-performance" liquid-chromatographic method. Additive and proportional differences between the methods were 0.07 mg/L and 9%, respectively, for procainamide and 0.62 mg/L and 16% for NAPA. Between-day CVs by the chromatographic and immunoassay methods, respectively, were 3.9% and 2.2% for procainamide at a concentration of 6 mg/L, and 5.1% and 1.2% for NAPA (14 mg/L). We applied a modification of the fluoroimmunoassay for determination of procainamide concentrations, using sera obtained during a pharmacokinetic study, and demonstrated excellent agreement with the chromatographic method.

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