scholarly journals Regulation of TCR δ and α repertoires by local and long-distance control of variable gene segment chromatin structure

2005 ◽  
Vol 202 (4) ◽  
pp. 467-472 ◽  
Author(s):  
Abbas Hawwari ◽  
Michael S. Krangel

Murine Tcrd and Tcra gene segments reside in a single genetic locus and undergo recombination in CD4−CD8− (double negative [DN]) and CD4+CD8+ (double positive [DP]) thymocytes, respectively. TcraTcrd locus variable gene segments are subject to complex regulation. Only a small subset of ∼100 variable gene segments contributes substantially to the adult TCRδ repertoire. Moreover, although most contribute to the TCRα repertoire, variable gene segments that are Jα proximal are preferentially used during primary Tcra recombination. We investigate the role of local chromatin accessibility in determining the developmental pattern of TcraTcrd locus variable gene segment recombination. We find variable gene segments to be heterogeneous with respect to acetylation of histones H3 and H4. Those that dominate the adult TCRδ repertoire are hyperacetylated in DN thymocytes, independent of their position in the locus. Moreover, proximal variable gene segments show dramatic increases in histone acetylation and germline transcription in DP thymocytes, a result of super long-distance regulation by the Tcra enhancer. Our results imply that differences in chromatin accessibility contribute to biases in TcraTcrd locus variable gene segment recombination in DN and DP thymocytes and extend the distance over which the Tcra enhancer can regulate chromatin structure to a remarkable 525 kb.

1998 ◽  
Vol 61 (2-3) ◽  
pp. 151-155 ◽  
Author(s):  
Yasuyoshi Kanari ◽  
Ryusuke Nakagawa ◽  
Hiroshi Arakawa ◽  
Hideo Yamagishi

1995 ◽  
Vol 42 (6) ◽  
Author(s):  
Bernhard Arden ◽  
StephenP. Clark ◽  
Dieter Kabelitz ◽  
TakW. Mak

1995 ◽  
Vol 41 (2-3) ◽  
Author(s):  
Juergen Bachl ◽  
Nadine Defranoux ◽  
Matthias Wabl

1988 ◽  
Vol 23 (2) ◽  
pp. 77-78
Author(s):  
E.J. Ball ◽  
K. Black ◽  
J.D. Capra ◽  
P. Stastny

2005 ◽  
Vol 57 (5) ◽  
pp. 352-363 ◽  
Author(s):  
Kathy S. Cho ◽  
Shi-Kang Zhai ◽  
Pedro J. Esteves ◽  
Katherine L. Knight

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