PENGARUH KEPEMIMPINAN TRANSFORMASIONAL, KEMAMPUAN MANAJERIAL, KARAKTERISTIK PEKERJAAN DAN MOTIVASI KERJA TERHADAP KINERJA PEGAWAI DINAS PEMUDA, OLAHRAGA, PARIWISATA DAN KEBUDAYAAN KABUPATEN BARITO SELATAN DI BUNTOK

Abstrak : Penelitian ini bertujuan untuk menganalisis Pengaruh Kepemimpinan Transformasional, Kemampuan Manajerial, Karakteristik Pekerjaan dan Motivasi Kerja terhadap Kinerja Pegawai Dinas Pemuda, Olahraga, Pariwisata dan Kebudayaan Kabupaten Barito Selatan di Buntok. Obyek penelitian adalah para pegawai Dinas Poraparbud sebanyak 67 pegawai. Penentuan sampel dalam penelitian ini menggunakan sampel jenuh. Teknik pengumpulan data menggunakan kuesioner, wawancara dan metode studi literatur. Analisis data yang digunakan menggunakan analisis regresi linier berganda. Hasil penelitian menunjukkan bahwa : (1) Motivasi Kerja berpengaruh secara dominan terhadap Kinerja pegawai Dinas Pemuda, Olahraga, Pariwisata dan Kebudayaan Kabupaten Barito Selatan hal ini telah terbukti dengan nilai beta sebesar 0,392 paling besar nilainya dibandingkan dengan nilai beta variable lainnya. (2) Secara simultan Kepemimpinan Transformasional (X1), Kemampuan Manajerial (X2), Karakteristik Pekerjaan (X3), dan Motivasi Kerja (X4) berpengaruh secara signifikan terhadap Kinerja Dinas Pemuda, Olahraga, Pariwisata dan Kebudayaan Kabupaten Barito Selatan. (3) Secara parsial Kepemimpinan Transformasional (X1), Kemampuan Manajerial (X2), Karakteristik Pekerjaan (X3), dan Motivasi Kerja (X4) berpengaruh secara signifikan terhadap Kinerja Dinas Pemuda, Olahraga, Pariwisata dan Kebudayaan Kabupaten Barito Selatan. Kata Kunci : Kepemimpinan transformasional, Kemampuan Manajerial, Karakteristik Pekerjaan, Motivasi Kerja, Kinerja Pegawai

Identification of a unique TCR repertoire, consistent with a superantigen selection process in Children with Multi-system Inflammatory Syndrome

SummaryMultisystem Inflammatory Syndrome in Children (MIS-C), a hyperinflammatory syndrome associated with SARS-CoV-2 infection, shares many clinical features with toxic shock syndrome, which is triggered by bacterial superantigens. The superantigen specificity for binding different Vβ-chains results in Vβ-skewing, whereby T cells with specific Vβ-chains and diverse antigen specificity are overrepresented in the TCR repertoire. Here, we characterized the TCR repertoire of MIS-C patients and found a profound expansion of TCR Beta Variable gene (TRBV)11-2. Furthermore, TRBV11-2 skewing was remarkably correlated with MIS-C severity and serum cytokine levels. Further analysis of TRBJ gene usage and CDR3 length distribution of MIS-C expanding TRBV11-2 clones revealed extensive junctional diversity, indicating a superantigen-mediated selection process for TRBV expansion. In silico modelling indicates that polyacidic residues in TCR Vβ11-2 engage in strong interactions with the superantigen-like motif of SARS-CoV-2 spike glycoprotein. Overall, our data indicate that the immune response in MIS-C is consistent with superantigenic activation.HighlightsMultisystem Inflammatory Disease in Children (MIS-C) patients exhibit T cell receptor (TCR) repertoire skewing, with expansion of T cell Receptor Beta Variable gene (TRBV)11-2TRBV11-2 skewing correlates with MIS-C severity and cytokine stormJ gene/CDR3 diversity in MIS-C patients is compatible with a superantigen selection processIn silico modelling indicates TCR Vβ11-2 engages in CDR3-independent interactions with the polybasic insert P681RRAR in the SAg-like motif of SARS-CoV-2 spike

Worldwide genetic variation of the IGHV and TRBV immune receptor gene families in humans

The immunoglobulin heavy variable (IGHV) and T cell beta variable (TRBV) loci are among the most complex and variable regions in the human genome. Generated through a process of gene duplication/deletion and diversification, these loci can vary extensively between individuals in copy number and contain genes that are highly similar, making their analysis technically challenging. Here, we present a comprehensive study of the functional gene segments in the IGHV and TRBV loci, quantifying their copy number and single-nucleotide variation in a globally diverse sample of 109 (IGHV) and 286 (TRBV) humans from over a 100 populations. We find that the IGHV and TRBV gene families exhibit starkly different patterns of variation. In addition to providing insight into the different evolutionary paths of the IGHV and TRBV loci, our results are also important to the adaptive immune repertoire sequencing community, where the lack of frequencies of common alleles and copy number variants is hampering existing analytical pipelines.

Haplotype Analysis of the TRB Locus by TCRB Repertoire Sequencing

Polymorphism within the T cell receptor beta variable gene (TRBV) has been implicated in autoimmune disease and immuneCrelated adverse events (IRAEs) during immunotherapy. Previous efforts to evaluate TRBV polymorphism by whole genome sequencing (WGS) have been hampered by the repetitive nature of the TCRB locus. We present a novel longCamplicon TCRB repertoire sequencing approach to evaluate TRBV polymorphism from peripheral blood, which we use to identify TRBV allele haplotypes in 81 Caucasians.