scholarly journals EFFECT OF INFECTION WITH M. TUBERCULOSIS AND OF TUBERCULIN SHOCK ON THE SUCCINIC DEHYDROGENASE ACTIVITY OF GUINEA PIG TISSUES

1953 ◽  
Vol 98 (2) ◽  
pp. 99-105 ◽  
Author(s):  
S. N. Chaudhuri ◽  
Samuel P. Martin
Keyword(s):  
Enzyme Activity ◽  
Guinea Pig ◽  
Dehydrogenase Activity ◽  
Guinea Pigs ◽  
Normal Tissue ◽  
Succinic Dehydrogenase ◽  
Tissue Extract ◽  
Succinic Dehydrogenase Activity ◽  
H37rv Strain ◽  
Sublethal Doses

The kidney of guinea pigs infected with the H37Rv and BCG strains of M. tuberculosis showed a diminution in succinic dehydrogenase activity when measured by the tetrazolium technique. This effect was also seen in the liver and spleen of animals infected with the BCG strain. Sensitized animals showed similar results when given tuberculin in sublethal doses. The succinic oxidase was also low in the kidneys of animals infected with the H37Rv strain. The depressed enzyme activity of the tissues of infected animals could be restored to normal by addition of normal tissue extract or dialysate. This suggests that the alteration in tissue metabolism observed in tuberculosis may depend upon the loss of some as yet unidentified factor important for succinic dehydrogenase activity.

Changes in succinic dehydrogenase activity related to feeding

1961 ◽  
Vol 201 (1) ◽  
pp. 37-40 ◽  
Author(s):  
R. R. Nielson ◽  
H. M. Klitgaard
Keyword(s):  
Enzyme Activity ◽  
Dehydrogenase Activity ◽  
Succinic Dehydrogenase ◽  
Feeding Period ◽  
Succinic Dehydrogenase Activity ◽  
Fasting Period ◽  
Psoas Muscles ◽  
Liver And Kidney ◽  
Effect Of Feeding ◽  
Feeding And Fasting

The effect of feeding and fasting on succinic dehydrogenase activity in rats accustomed to a feeding regime (fed 5 hr/24 hr) was studied over a 24-hr period and over a subsequent extended fast. The results showed a fall in enzyme activity to a minimal value at 6 hr after the initial feeding period in the four tissues studied. During the 24-hr period following feeding, cardiac and psoas muscles obtained maximum values which were 134% and 174%, respectively, of the 6-hr value at 18 hr. Liver and kidney attained their maximum values (133% and 174%, respectively) during the second 5-hr feeding period. When the fasting period was extended by omitting the second feeding period, liver and kidney tended to plateau (after their peak at 24 hr) near the 6-hr assay, whereas cardiac and psoas muscles (after their peaks at 24 and 18 hr, respectively) tended to plateau with 145% of the 6-hr activity. Variations related to feeding times, not previously realized, were demonstrated.

SUCCINIC DEHYDROGENASE ACTIVITY OF THE THYROID GLAND AS AN INDICATOR OF THE ACTIVITY OF THE THYROID CELLS

1960 ◽  
Vol XXXV (I) ◽  
pp. 135-138 ◽  
Author(s):  
Antti Telkkä ◽  
K. J. Heikkilä ◽  
Väinö K. Hopsu
Keyword(s):  
Thyroid Gland ◽  
Enzymatic Activity ◽  
Dehydrogenase Activity ◽  
Guinea Pigs ◽  
Quantitative Estimation ◽  
Succinic Dehydrogenase ◽  
Succinic Dehydrogenase Activity ◽  
Thyroid Cells ◽  
Thyroid Glands ◽  
Sensitive Method

ABSTRACT The thyroid glands of guinea-pigs were stimulated by the administration of methylthiouracil. The histochemically demonstrable succinic dehydrogenase activity was determined by the quantitative tetrazolium method. A marked increase in the enzymatic activity of the stimulated thyroid glands was observed and measured. The quantitative estimation of enzymatic activity is apparently a reliable and sensitive method for demonstrating the activity of the thyroid cells.

Succinic dehydrogenase activity in the skin of the guinea pig

1954 ◽  
Vol 120 (4) ◽  
pp. 893-905 ◽  
Author(s):  
Victor R. Formisano ◽  
William Montagna
Keyword(s):  
Guinea Pig ◽  
Dehydrogenase Activity ◽  
Succinic Dehydrogenase ◽  
Succinic Dehydrogenase Activity

Histochemistry of Hymenolepis microstoma (Cestoda: Hymenolepididae)

Parasitology ◽  
1966 ◽  
Vol 56 (1) ◽  
pp. 55-61 ◽  
Author(s):  
Burton J. Bogitsh ◽  
David A. Nunnally
Keyword(s):  
Enzyme Activity ◽  
Dehydrogenase Activity ◽  
Succinic Dehydrogenase ◽  
Anterior Region ◽  
Succinic Dehydrogenase Activity ◽  
Blue Tetrazolium ◽  
High Concentrations ◽  
Intense Activity ◽  
Cryostat Sections ◽  
Hymenolepis Microstoma

Reduction of neotetrazolium in the presence of succinate is used as a quantitative assay for succinic dehydrogenase activity in homogenates of Hymenolepis microstoma, and nitro blue tetrazolium and thiazolyl blue tetrazolium-cobalt are employed with cryostat sections for its histochemical localization in adults and cysticercoids. The highest concentrations of the enzyme occur in the anterior region of the worm (scolex, neck, and immature proglottids), lesser amounts in the region of mature and early gravid segments, and least amounts in the gravid region of the strobila. Primary sites of succinic dehydrogenase activity are the mitochondrial layer of the cuticle and the cells of the subcuticle. In the anterior region the entire parenchyma shows uniformly high concentrations of enzyme activity. This reaction decreases posteriorly until, in the gravid region, only the subcuticle and the mitochondrial layer of the cuticle display activity. Intense activity was also noted in the layer just under the shells of the eggs. The ovary and immature testes show high enzyme concentrations; however, in the testes, the concentration diminishes as the organs mature. In the scolex, the rostellum and the muscles of the suckers show higher enzyme activity than the surrounding tissues. The scolex, inner membrane, and tail of the cysticercoid show uniformly high concentrations of formazan.

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