Development of Competitive Enzyme-Linked Immunosorbent Assays for Antibody Detection Based on Bluetongue Virus Monoclonal Antibodies

2018 ◽  
Vol 31 (3) ◽  
pp. 264-271 ◽  
Author(s):  
Zhou Han ◽  
Zang Mingxin ◽  
Li Xuechun ◽  
Xu Yigang ◽  
Qiao Xinyuan ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Bluetongue Virus ◽  
Antibody Detection ◽  
Immunosorbent Assays

Monoclonal Antibody Detection of Porcine Meat

1994 ◽  
Vol 57 (2) ◽  
pp. 146-149 ◽  
Author(s):  
P. MORALES ◽  
T. GARCÍA ◽  
I. GONZÁLEZ ◽  
R. MARTÍN ◽  
B. SANZ ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Bovine Serum Albumin ◽  
Horseradish Peroxidase ◽  
Indirect Elisa ◽  
Cross Reactivity ◽  
Antibody Detection ◽  
Muscle Proteins ◽  
Elisa Format ◽  
Soya Proteins

A stable hybridoma cell line (DD9) has been produced secreting a monoclonal antibody specific for porcine muscle proteins. The DD9 monoclonal antibody (mAb) failed to show a significant cross-reactivity when tested against beef, horse, chicken, and soya proteins, as well as bovine caseins, gelatin, and bovine serum albumin. The DD9 mAb was further used in an indirect ELISA format for detection of defined amounts of porcine meat (1–100%) in beef and chicken meat mixtures immobilized on 96-well plates. Immunorecognition of monoclonal antibodies adsorbed to porcine meat was made with rabbit anti-mouse immunoglobulins conjugated to the enzyme horseradish peroxidase.

Monoclonal Antibodies Against VP7 of Bluetongue Virus

Hybridoma ◽  
2012 ◽  
Vol 31 (6) ◽  
pp. 469-472 ◽  
Author(s):  
Wen-Shi Wang ◽  
En-Cheng Sun ◽  
Ni-Hong Liu ◽  
Tao Yang ◽  
Qing-Yuan Xu ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Bluetongue Virus

Detection of Escherichia coli cytotoxins by enzyme-linked immunosorbent assays

1991 ◽  
Vol 37 (8) ◽  
pp. 650-653 ◽  
Author(s):  
Joan I. Speirs ◽  
Mumtaz Akhtar
Keyword(s):  
Escherichia Coli ◽  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Vero Cell ◽  
Key Words ◽  
E Coli ◽  
Treated Cell ◽  
Cell Extracts ◽  
Immunosorbent Assays ◽  
Key Words Escherichia Coli

Sandwich enzyme-linked immunosorbent assays (ELISAs) were developed to detect Escherichia coli cytotoxins. Wells were coated with monoclonal antibodies from hybridomas 13C4 and (or) 11E10, and biotin conjugates of these antibodies were used for detecting verotoxin 1 and Shiga-like toxin II, respectively. Sensitivities were about 100 and 200 cytotoxic doses, respectively. Verotoxin 2 was detected by ELISA with monoclonal antibody 11E10, but at a sensitivity of only about 4000 cytotoxic doses. ELISA results of polymyxin-treated cell extracts from cultures of 67 E. coli strains were in agreement with Vero cell assay as regards the presence and type of toxin. Key words: Escherichia coli, cytotoxin, ELISA.

Identification of antigenic epitopes of monoclonal antibodies against the VP2 protein of the 25 serotype of bluetongue virus

2018 ◽  
Vol 219 ◽  
pp. 136-143 ◽  
Author(s):  
Shuangyu Xie ◽  
Yiming Shi ◽  
Ruyue Gong ◽  
Wen Cui ◽  
Yanping Jiang ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Bluetongue Virus ◽  
Vp2 Protein ◽  
Antigenic Epitopes
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