Spectrophotofluorometric Determination of Porphyrins in Urine

1971 ◽  
Vol 17 (3) ◽  
pp. 199-205 ◽  
Author(s):  
Carolyn A Martinez ◽  
Gordon C Mills
Keyword(s):  
Anion Exchange ◽  
Coefficient Of Variation ◽  
Exchange Resin ◽  
Anion Exchange Resin ◽  
Urine Samples ◽  
Rapid Procedure ◽  
Normal Urine ◽  
Urine Specimens

Abstract A rapid procedure has been developed in which an anion-exchange resin and spectrophotofluorometry are used to determine total porphyrins in urine. After porphyrins are eluted from the resin with 3N HCl, their fluorescence at 646 nm is measured with an Aminco-Bowman Spectrophotofluorometer. About 10 min are required per analysis by a skilled technician. Added to urine specimens, 95.3 ± 4.9% of uroporphyrin and 94.7 ± 9.6% of coproporphyrin, were accounted for. Eighteen normal urine samples contained an average of 0.063 µmol of porphyrin per liter (range, 0.028-0.176 µmol/liter). The coefficient of variation, 2.9%, indicates the precision to be very satisfactory.

Quantitative Determination of FD&C Colors in Foods

1975 ◽  
Vol 58 (2) ◽  
pp. 278-282
Author(s):  
Charles Graichen
Keyword(s):  
Quantitative Determination ◽  
Anion Exchange ◽  
Salt Solution ◽  
Exchange Resin ◽  
Anion Exchange Resin ◽  
Collaborative Study ◽  
Chromatographic Technique ◽  
Different Color ◽  
Selection Of

Abstract A published procedure using Amberlite LA-2 (a liquid anion exchange resin) solutions to extract colors from food and the salt solution cellulose chromatographic technique to separate colors from each other were updated and submitted to collaborative study. Three batches of cookies and 4 purchased products were analyzed by 13 collaborators. The 3 batches of cookies contained different color mixtures requiring the selection of various procedures. The 4 purchased products were selected principally to include a variety of foods. Some results were partially deficient. The deficiencies reflect certain inadequacies in the directions as written rather than basic flaws in the method. Some improvements were suggested by the collaborators. The Associate Referee recommends that the method be further revised to correct the present inadequacies and to include some improvements, and that the revised method be submitted to another collaborative study.

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