Blood Glucose Determination without Deproteinization, with Use of o-Toluidine in Dilute Acetic Acid

1971 ◽  
Vol 17 (5) ◽  
pp. 440-441 ◽  
Author(s):  
Giovanni Ceriotti

Abstract A method is described for determining glucose in 25 µl of plasma. o-Toluidine is used in weak acetic acid solution. Addition of the emulsifier "Cremophor" obviates the need for deproteinization. The reaction is complete after 15 min at 100°C, and the color is stable for longer than 24 h. The blank is almost colorless. The reagent is stable, colorless, not viscous, and convenient to handle.

1962 ◽  
Vol 8 (6) ◽  
pp. 592-592 ◽  
Author(s):  
Kurt M Dubowski

Abstract Since publication of the above paper, we have found that a procedure similar to the one described for determination of aldosaccharides in body fluids using o-toluidine in glacial acetic acid solution was described by Eric Hultman in Nature183, 108 (1959). A modification of Hultman's procedure has recently been described by A. Hyvärinen and E. A. Nikkilä in Clin. Chim. Acta7, 140 (1962). The omission of reference to Dr. Hultman's paper was entirely unintentional. Our own procedure was, of course, derived entirely experimentally, based upon Dr. O. M. Forsell's suggestion to us of Mar. 13, 1960, that we should consider the use of o-toluidine as a glucose reagent; and this suggestion is properly credited in the paper as a personal communication. The papers by Hultman and by Hyvärinen and Nikkilä nicely confirm some of the significant experimental details and results of our procedure; but obviously the Hultman paper has priority with respect to this principle.


2016 ◽  
Vol 10 (2) ◽  
pp. 135-139 ◽  
Author(s):  
Valentina Chernova ◽  
◽  
Angela Shurshina ◽  
Elena Kulish ◽  
Gennady Zaikov ◽  
...  

Some ways of estimating the values of the intrinsic viscosity of chitosan were analyzed. It was shown that the method of Irzhak and Baranov for estimating the current value of the intrinsic viscosity allows to adequately estimates the conformational state of the macromolecular coil and its degree of swelling.


2010 ◽  
Vol 82 (2) ◽  
pp. 251-255 ◽  
Author(s):  
H.M.P. Naveen Kumar ◽  
M.N. Prabhakar ◽  
C. Venkata Prasad ◽  
K. Madhusudhan Rao ◽  
T.V. Ashok Kumar Reddy ◽  
...  

1944 ◽  
Vol 66 (10) ◽  
pp. 1752-1755 ◽  
Author(s):  
Herbert S. Anker ◽  
Konrad Bloch

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