Rapid Single-Step Kinetic Colorimetric Assay for Lactate Dehydrogenase in Serum

1973 ◽  
Vol 19 (2) ◽  
pp. 223-227 ◽  
Author(s):  
Charles C Allain ◽  
Carl P Henson ◽  
M Keith Nadel ◽  
Adam J Knoblesdorff
Keyword(s):  
Lactate Dehydrogenase ◽  
Dehydrogenase Activity ◽  
Dynamic Range ◽  
Colorimetric Assay ◽  
Single Step ◽  
Tetrazolium Salt ◽  
Lactate Dehydrogenase Activity ◽  
Tetrazolium Chloride ◽  
System A ◽  
Reducing Substances

Abstract We report an improved kinetic colorimetric system for measuring lactate dehydrogenase activity in serum. In the system a tetrazolium salt, 2-p-iodophenyl-3-p-nitrophenyl-5-phenyl tetrazolium chloride, is used as the chromogenic indicator of dehydrogenase activity, with diaphorase serving as the electron transfer agent. All ingredients required for an assay are combined in a single dry reagent that is stable at room temperature. The method is 2.5 times as sensitive as the ultraviolet method of Wacker and has a dynamic range three times that of the ultraviolet method. Reducing substances in serum do not affect the results. Precision, range of linearity, and stability of reagent after reconstitution are excellent. Results for fresh sera correlated well with those obtained by the "A-Gent" ultraviolet method (Wacker method at 37°C) and with the SMA 12/60.

Kinetic Colorimetric Measurement of Serum Lactate Dehydrogenase Activity

1973 ◽  
Vol 19 (7) ◽  
pp. 766-769 ◽  
Author(s):  
Arthur L Babson ◽  
Susan R Babson
Keyword(s):  
Lactate Dehydrogenase ◽  
Dehydrogenase Activity ◽  
Colorimetric Assay ◽  
Molar Absorptivity ◽  
Serum Lactate ◽  
Serum Lactate Dehydrogenase ◽  
Tetrazolium Salt ◽  
Kinetic Assay ◽  
Lactate Dehydrogenase Activity ◽  
Tetrazolium Chloride

Abstract A kinetic colorimetric assay procedure is described for measuring lactate dehydrogenase activity, in which the reduction of NAD is coupled to the reduction of a tetrazolium salt, 2-p-iodophenyl-3-p-nitrophenyl-5-phenyl tetrazolium chloride (INT), with phenazine methosulfate serving as an intermediate electron carrier. The molar absorptivity of the formazan of INT was found to be 19.3 x 103 at 503 nm, which provides a threefold increase in sensitivity over the ultraviolet (340 nm) kinetic assay. The results correlate well with those obtained by 340-nm kinetic methods and the procedure does not require an ultraviolet spectrophotometer. The method is simple, rapid, and does not rely on secondary enzyme standards. It requires only two reagents, which are stable.

Lactate dehydrogenase activity and its isoenzymes in concentric layers of adult bovine and calf lenses

1987 ◽  
Vol 6 (4) ◽  
pp. 555-560 ◽  
Author(s):  
David Sempol ◽  
Edurado Osinaga ◽  
Seymour Zigman ◽  
Israel Korc ◽  
Beatriz Korc ◽  
...  
Keyword(s):  
Lactate Dehydrogenase ◽  
Dehydrogenase Activity ◽  
Lactate Dehydrogenase Activity

Lactate Dehydrogenase Activity is Inhibited by Methylmalonate in vitro

2006 ◽  
Vol 31 (4) ◽  
pp. 541-548 ◽  
Author(s):  
Laura O. Saad ◽  
Sandra R. Mirandola ◽  
Evelise N. Maciel ◽  
Roger F. Castilho
Keyword(s):  
Lactate Dehydrogenase ◽  
Dehydrogenase Activity ◽  
Lactate Dehydrogenase Activity

scholarly journals CSF Lactate Dehydrogenase Activity in Patients with Creutzfeldt-Jakob Disease Exceeds That in Other Dementias

2004 ◽  
Vol 17 (3) ◽  
pp. 204-206 ◽  
Author(s):  
H. Schmidt ◽  
M. Otto ◽  
P. Niedmann ◽  
L. Cepek ◽  
A. Schröter ◽  
...  
Keyword(s):  
Lactate Dehydrogenase ◽  
Dehydrogenase Activity ◽  
Lactate Dehydrogenase Activity ◽  
Csf Lactate ◽  
Jakob Disease
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