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2022 ◽  
Vol 17 (1) ◽  
Author(s):  
Lei Sun ◽  
Jia-min Chen ◽  
Kun Yang ◽  
Liang Zhang ◽  
Zhi-yuan Ma ◽  
...  

Abstract Background Cytomegalovirus (CMV) has been recognized as one of the frequently occurring opportunistic infections (OIs) reported in the patients having human immunodeficiency virus/acquired immunodeficiency syndrome (HIV/AIDS). In addition, it has been identified as the factor leading to gastrointestinal (GI) tract disorder among HIV/AIDS population. CMV exhibits broad cell tropism in different organs. This study evaluated the CMV cell tropism and clinicopathological characteristics of CMV infection in the different GI regions in HIV/AIDS cases. Methods Using nucleic acid in situ hybridization (ISH), CMV was detected in the gastrointestinal mucosal biopsy samples. The paraffin-embedded samples were stained with hematoxylin and eosin (HE) and immunohistochemistry (IHC), respectively. Results A total of 32 HIV/AIDS patients were enrolled in this study. Fourteen of these patients underwent gastroscopy, while the remaining eighteen received colonoscopy. CMV-infected cells were observed at 46 GI sites. Among them, the colon was the region with the highest susceptibility to GI CMV infection (n = 12, 26.1%). The CMV giant cell inclusion bodies were detected in epithelial cells and mesenchymal cells, including histiocytes, smooth muscle cells, fibroblasts, and endothelial cells. In the duodenum, there were markedly more positive epithelial cells than mesenchymal cells (p = 0.033). In contrast, in the esophagus (p = 0.030), cardia (p = 0.003), rectum (p = 0.019), colon (p < 0.001), and cecum (p < 0.001), there were notably less positive epithelial cells than mesenchymal cells. The expression levels of PDGFRα and Nrp2 in the mesenchymal cells were higher than the epithelial cells in cardia, cecum, colon, sigmoid, and rectum, especially in the areas with ulcers. However, Nrp2 in the epithelial cells was higher than that in the duodenum. Moreover, the positive CMV DNA in peripheral blood was related to the CMV-positive cell count, as well as the ulceration in GI tract (p = 0.035 and 0.036, respectively). Conclusions The colon has been identified as the GI site with the highest susceptibility to CMV infection. There are different CMV-infected cells in the different sites of the GI that relate to the expression level of PDGFRα and Nrp2. CMV DNA positive in the blood is related to the positive CMV cell count, as well as ulceration in the GI tract.


Author(s):  
Karam Mounzer ◽  
Laurence Brunet ◽  
Jennifer S Fusco ◽  
Ian R Mcnicholl ◽  
Helena Diaz Cuervo ◽  
...  

Abstract Background Approximately 20% of newly diagnosed people with HIV (PWH) in the U.S. have advanced HIV infection, yet literature on current antiretroviral therapy (ART) options is limited. Discontinuation/modification and effectiveness of common regimens were compared among ART-naïve people with advanced HIV infection (CD4 cell count &lt;200 cells/μL). Methods ART-naïve adults with advanced HIV infection initiating bictegravir/emtricitabine/tenofovir alafenamide (B/F/TAF) or a boosted darunavir (bDRV)-, dolutegravir (DTG)- or elvitegravir/cobicistat (EVG/c)-based three-drug regimen between 1JAN2018 and 31JUL2019 in the OPERA cohort were included. The association between regimen and discontinuation or viral suppression (&lt;50 or &lt;200 copies/mL) was assessed using Cox proportional hazards models with inverse probability of treatment weights. Results Overall, 961 PWH were included (416 B/F/TAF, 106 bDRV, 271 DTG, 168 EVG/c); 70% achieved a CD4 cell count ≥200 cells/μL over a 16 months median follow-up. All regimens were associated with a statistically higher likelihood of discontinuation than B/F/TAF (bDRV aHR: 2.65 [95% CI: 1.75, 4.02], DTG: 2.42 [1.75, 3.35], EVG/c: 3.52 [95% CI: 2.44, 5.07]). Compared to B/F/TAF, bDRV initiators were statistically less likely to suppress to &lt;50 copies/mL (0.72 [0.52, 0.99]) and &lt;200 copies/mL (0.55 [0.43, 0.70]); no statistically significant difference was detected with DTG or EVG/c. Conclusions Among people with advanced HIV infection, those initiating B/F/TAF were less likely to discontinue/modify their regimen than those on any other regimen, and more likely to achieve viral suppression compared to those on bDRV but not compared to those on other integrase inhibitors.


2022 ◽  
pp. 000313482110540
Author(s):  
Jae Hee Cho ◽  
M Jason Akers ◽  
Mehrnaz Siavoshi ◽  
Todd Gress ◽  
Errington C Thompson

Background: The purpose of this study is to investigate the relevant findings in adult patients admitted to Cabell Huntington Hospital who were diagnosed with acute appendicitis. Methods: Patients who had the postoperative diagnosis of acute appendicitis and a preoperative computed tomography (CT) scan from January 2011 through December 2016 were included in this retrospective chart review. Results: There were 592 patients. A thick, edematous appendix was the most common CT finding in acute appendicitis. The average diameter was 12.6 mm. The wall thickness correlated to the diameter of the appendix ( P < 0.001). For comparison, we reviewed the CT scans of 50 trauma patients who had normal abdominal CT scans. The average diameter of a normal appendix was 4.9 mm (SD 1.139) with a range of 4-7 mm. Interestingly, the admission white blood cell count ( P = 0.0372) as well as the thickness of the appendix ( P < 0.0001) were strongly associated with increased length of stay. Conclusions: An appendiceal diameter greater than 9 mm should be considered abnormal and associated with acute appendicitis. Appendiceal size, white blood cell count, and age correlate with length of stay. Early antibiotics and early surgical intervention may decrease length of stay.


2022 ◽  
Vol 2022 ◽  
pp. 1-6
Author(s):  
Sergei Yu. Zaitsev ◽  
Oksana A. Voronina ◽  
Anastasia A. Savina ◽  
Larisa P. Ignatieva ◽  
Nadezhda V. Bogolyubova

The aim of the work was to study the correlations between the total amount of water-soluble antioxidants (TAWSA) and biochemical parameters (BC) of cow milk depending on the somatic cell count (SCC). The BC and TAWSA values of cow milk were measured by spectroscopic and amperometric methods, respectively. The milk samples from the black-and-white cows (Moscow region) were divided according to SCС values: (1) ≤200, (2) 200-499, (3) 500-999, and (4) ≥1000 thousand units/mL. The average TAWSA values for groups 1, 2, 3, and 4 (33, 15, 13, and 12 milk samples) were the following: 15.95 ± 0.74 , 14.45 ± 0.84 , 16.04 ± 0.63 , and 14.58 ± 1.18 . The correlations between TAWSA and BC (group 1) were the following: total fat percentage (TFP) -0.305; true protein percentage (TP1) -0.197; total nitrogen percentage (TN2) -0.210; lactose -0.156; solids-not-fat (SNF) -0.276; total dry matter (TDM) -0.399; freezing point (FP) -0.112; pH -0.114; somatic cell count (SCC) - (-0,052). The correlations between TAWSA and BC (group 2) were the following: TFP -0.332; TP1 -0.296; TN2 -0.303; lactose - (-0.308); SNF -0.159; TDM -0.391; FP -0.226; pH - (-0.211); SCC -0.193. The correlations between TAWSA and BC (group 3) were the following: TFP - (-0.352); TP1 - (-0.411); TN2 – (-0.401); lactose - (-0.166); SNF - (-0.462); TDM - (-0.504); FP - (-0.766); pH - (-0.047); SCC - (-0.698). The correlations between TAWSA and BC (group 4) were the following: TFP -0.159; TP1 -0.046; TN2 – 0.077; lactose - (-0.317); SNF - (-0.237); TDM -0.058; FP - (-0.036); pH - (-0.477); SCC - (-0.072). These data are important in assessing the physiological-biochemical status and state of the antioxidant defense system of cows’ organism.


2022 ◽  
Vol 2022 ◽  
pp. 1-13
Author(s):  
Sarang Sharma ◽  
Sheifali Gupta ◽  
Deepali Gupta ◽  
Sapna Juneja ◽  
Punit Gupta ◽  
...  

Blood cell count is highly useful in identifying the occurrence of a particular disease or ailment. To successfully measure the blood cell count, sophisticated equipment that makes use of invasive methods to acquire the blood cell slides or images is utilized. These blood cell images are subjected to various data analyzing techniques that count and classify the different types of blood cells. Nowadays, deep learning-based methods are in practice to analyze the data. These methods are less time-consuming and require less sophisticated equipment. This paper implements a deep learning (D.L) model that uses the DenseNet121 model to classify the different types of white blood cells (WBC). The DenseNet121 model is optimized with the preprocessing techniques of normalization and data augmentation. This model yielded an accuracy of 98.84%, a precision of 99.33%, a sensitivity of 98.85%, and a specificity of 99.61%. The proposed model is simulated with four batch sizes (BS) along with the Adam optimizer and 10 epochs. It is concluded from the results that the DenseNet121 model has outperformed with batch size 8 as compared to other batch sizes. The dataset has been taken from the Kaggle having 12,444 images with the images of 3120 eosinophils, 3103 lymphocytes, 3098 monocytes, and 3123 neutrophils. With such results, these models could be utilized for developing clinically useful solutions that are able to detect WBC in blood cell images.


2022 ◽  
Vol 43 (1) ◽  
pp. 141-158
Author(s):  
Mauricio Fanin ◽  
◽  
Isabela Carvalho dos Santos ◽  
Geysiane Moreira Gerotti ◽  
Camila de Cuffa Matusaiki ◽  
...  

Milk and its derivatives are highly consumed foods worldwide, with recognized nutritional importance. The search for the production of products with superior quality is constant. For the present work, 26 milk-producing properties were selected, with a total of 506 milk samples collected during the period from October 2019 to May 2020 being evaluated. The objective of this study was to evaluate the quality of milk produced in dairy properties in the region west Paraná, classified as good or bad based on the results of the Somatic Cell Count (SCC) and through sampling (n = 10) to evaluate the resistance profile of enterobacteria and Staphylococcus spp. isolated from milk samples, in addition to the presence of the mecA gene in strains of Staphylococcus spp. resistant to oxacillin. There were significant differences between the good and bad properties for the levels of lactose, SCC (cell/mL), and Standard Plate Count (SPC) (CFU/mL). The strains of Staphylococcus spp. showed differences in the percentage of resistance in relation to the good and bad properties for antibiotics: tetracycline, ciprofloxacin, oxacillin, amikacin, clindamycin, gentamycin, and erythromycin. The mecA gene was not detected in any of the coagulase-negative Staphylococcus isolates that showed resistance to oxacillin. For enterobacteria, the isolated species differed in relation to the classification of properties, with predominance for Escherichia coli (40%) for properties classified as bad and Hafnia alvei (40%) for those classified as good. The percentage of antibiotic resistance compared to enterobacteria isolates was higher in properties classified as good. Monitoring through microbial culture and antibiogram is extremely important, favoring the correct choice for the treatment of animals with a reduced selection of resistant strains.


2022 ◽  
pp. 112067212110709
Author(s):  
Bilgehan Sezgin Asena ◽  
Mahmut Kaskaloglu

Purpose To evaluate efficacy and safety of contact lens-assisted corneal cross-linking (CACXL) among progressive keratoconus patients with thin corneas. Setting Private eye hospital Design Cross-sectional study Methods Thirty-six eyes of 36 consecutive patients with progressive keratoconus and thinnest corneal thickness of 380–400 µm after epithelium removal were included. CACXL was performed based on use of a dextran-free isotonic (>0.1%) riboflavin solution with hydroxypropyl methylcellulose (HPMC, 1.1%) after epithelial removal and placement of a riboflavin-soaked contact lens without an ultraviolet filter on the cornea and ultraviolet irradiance. Data on best distance corrected (CDVA) and uncorrected (UDVA) visual acuity, manifest sphere (D), manifest cylinder (D), K1 (D), K2 (D), mean keratometry (D) and Kmax (D) values, endothelial cell count and mean depth of demarcation line were recorded preoperatively and at the postoperative 1-year Results Post-operative 1-year data revealed significant increases in UDVA (0.23  ±  0.15 vs. 0.17  ±  0.13 D, p < 0.001) and CDVA (0.44  ±  0.18 vs. 0.36  ±  0.17 D, p < 0.001) values and a significant reduction in the mean Kmax (from 56  ±  3.3D to 55  ±  3.2 D, p < 0.001), K2 (from 49.7  ±  3.2 D to 49.52  ±  3.11 D, p  =  0.049), manifest sphere (from −1.93  ±  2.21 D to −1.55  ±  2.02 D, p  =  0.001) and manifest cylinder (from −2.83  ±  1.67 D to −2.39  ±  1.36 D, p < 0.001) values along with a similar endothelial cell count. The mean depth of demarcation line was mean 230 (SD17.05, range 200 to 262) at postoperative 1-month. Conclusions Our findings indicate favourable 1-year postoperative outcome of CACXL in progressive keratoconus patients with thin cornea in terms of improved visual acuity and keratectasia status as well as endothelial safety.


Cancers ◽  
2022 ◽  
Vol 14 (2) ◽  
pp. 325
Author(s):  
Maciej Majcherek ◽  
Agnieszka Matkowska-Kocjan ◽  
Donata Szymczak ◽  
Magdalena Karasek ◽  
Agnieszka Szeremet ◽  
...  

Vaccination against SARS-CoV-2 is currently the best tool in the fight against the COVID-19 pandemic. However, there are limited data on its efficacy and safety after hematopoietic stem cell transplantation (HCT). We present the results of a prospective analysis of the humoral response to two doses of BNT162b2 mRNA vaccine in 93 adult patients, including 29 after autologous HCT (autoHCT) and 64 after allogeneic HCT (alloHCT). Positive anti-SARS-CoV-2 antibodies were detected before vaccination in 25% of patients despite a negative medical history of COVID-19. Seroconversion after vaccination was achieved in 89% of patients after alloHCT and in 96% after autoHCT, without grade 3/4 adverse events. Post-vaccination anti-SARS-CoV-2 antibody level correlated with the time from transplant and absolute B-cell count at the vaccination. In univariate analysis restricted to the alloHCT group, short time since transplantation, low B-cell count, low intensity conditioning, GvHD, and immunosuppressive treatment at the vaccination were associated with lack of seroconversion. In the multivariate model, the only negative predictor of seroconversion remained treatment with calcineurin inhibitor (CNI). In conclusion, the BNT162b2 mRNA vaccine is highly immunogenic in patients after HCT, but treatment with CNI at the time of vaccination has a strong negative impact on the humoral response


2022 ◽  
Vol 2022 ◽  
pp. 1-6
Author(s):  
Tian Qin ◽  
Wang Sheng ◽  
Guoheng Hu

To analyze the influencing factors of senile coronary heart disease patients complicated with frailty syndrome. A total of 80 elderly patients with coronary heart disease admitted to our hospital from March 2020 to March 2021 were selected as the research subjects. The Fried Frailty Symptom Scale was used to evaluate whether the 80 patients were complicated with frailty syndrome. According to the evaluation results, the patients were divided into a nonfrailty syndrome group (52 cases in total) and frailty syndrome group (28 cases in total). Clinical data of two groups of patients were collected, and multivariate logistic regression was used to analyze the influencing factors of senile coronary heart disease patients complicated with frailty syndrome. Among 80 patients, the incidence of frailty syndrome was 35.00% (28/80), including 18 cases in early frailty and 10 cases in frailty stage. Univariate analysis showed that age, body mass (BMI), diabetes mellitus, congestive heart failure, chronic renal insufficiency, chronic obstructive pulmonary disease (COPD), tumor, high uric acid hematic disease, arrhythmia, interleukin-6 (IL-6), c-reactive protein (CRP), fibrinogen (FIB), brain natriuretic peptide (BNP), uric acid (UA), serum creatinine (Scr), serum protein (ALB), white blood cell count (WBC), and neutrophil count were the possible risk factors for senile coronary heart disease complicated with frailty syndrome ( P  < 0.05). Multivariate logistic regression analysis showed that combined COPD, combined tumor, IL-6, BNP, UA, SCR, ALB, and neutrophil count were independent risk factors for senile CHD complicated with frailty syndrome ( P  < 0.05). Combined with COPD, combined with tumor, IL-6, BNP, UA, SCR, ALB, and neutron cell count are the influencing factors for senile coronary heart disease patients complicated with frailty syndrome. These factors can be used as the basis for the diagnosis of frailty syndrome and guide the clinical development of targeted diagnosis and treatment plan.


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