Immunoglobulin Concentrations in Sera of Normal Children: Quantitation against an International Reference Preparation

1974 ◽  
Vol 20 (6) ◽  
pp. 656-659 ◽  
Author(s):  
Jan Čejka ◽  
Darlene W Mood ◽  
Chung Su Kim
Keyword(s):  
Human Serum ◽  
Healthy Children ◽  
Normal Children ◽  
International Reference ◽  
Serum Immunoglobulins ◽  
Reference Preparation

Abstract Immunoglobulins G, A, and M were quantitated in sera from healthy children of various ages by using a standard related to the international reference preparation for human serum immunoglobulins. The results, expressed in International Units per milliliter, provide a basis for the establishment of normal immunoglobulin values in different laboratories.

Standardization for four protein analytes with the Behring Nephelometer.

1988 ◽  
Vol 34 (9) ◽  
pp. 1870-1872 ◽  
Author(s):  
S B Schotters ◽  
J H McBride ◽  
D O Rodgerson ◽  
S Higgins ◽  
M Pisa
Keyword(s):  
Human Serum ◽  
World Health Organization ◽  
Serum Proteins ◽  
World Health ◽  
International Reference ◽  
The World ◽  
Reference Preparation ◽  
Primary Standards ◽  
Health Organization ◽  
Ig G

Abstract Owing to the generally higher values observed in the initial establishment of immunoglobulins (Ig) G, A, and M assays with the Behring Nephelometer, we elected to verify five commercial protein calibrators. This initial verification was performed by standardizing the Behring Nephelometer with the World Health Organization (WHO) International Reference Preparation for Human Serum Immunoglobulins G, A, and M. The instrument was also standardized for immunoglobulins and transferrin with use of the Reference Preparation for Serum Proteins (RPSP II). Analytical recoveries of the commercial calibrators varied. Also, assigned protein concentrations in both the WHO and RPSP II preparations made them unacceptable to us as benchmark calibrators for the Behring Nephelometer. Individual proteins (IgG, IgA, IgM, and transferrin) were obtained and primary standards prepared. Both transferrin and IgG were successfully standardized. However, IgA and IgM primary standards lacked 100% antigenicity, requiring removal of nonreactive IgA and IgM or reassignment of the correct value. The problem remains to find appropriate purified materials for IgA and IgM standardization.

THE AUSTRALIAN REFERENCE PREPARATION OF HUMAN SERUM IMMUNOGLOBULINS

1980 ◽  
Vol 58 (4) ◽  
pp. 351-356 ◽  
Author(s):  
KJ Turner ◽  
PM Carter ◽  
CS Hosking ◽  
WK Finger
Keyword(s):  
Human Serum ◽  
Serum Immunoglobulins ◽  
Reference Preparation
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