Concentrations of atrial natriuretic peptide in plasma and urine of kidney disease patients

Abstract Concentrations of human atrial natriuretic peptide-like immunoreactivity (hANP-LI) were measured by a highly sensitive and specific radioimmunoassay (Biochem Biophys Res Commun 1986;137:231-6) in normal subjects and in renal disease patients without accompanying congestive heart failure, hypertension, edema, diabetes, or pregnancy. We attempted to clarify whether the hANP-LI concentration in plasma was increased by loss of renal mass. We found no correlation between the hANP-LI concentration in plasma and creatinine clearance (Ccr, 4.6-122.3 mL/min) in patients with renal disease (n = 63, r = -0.196), nor between hANP-LI concentrations in plasma and urine (n = 97, r = -0.207). The fractional excretion of hANP (FEhANP) correlated significantly with Ccr (n = 63, r = 0.520, P less than 0.01) and with FENa (n = 35, r = -0.503, P less than 0.01). Increased FEhANP in patients with chronic renal failure may have resulted because of an increase in single-nephron glomerular filtration rate similar to the FENa increase in these patients. The present data indicate that decreased renal function itself does not increase the concentration of hANP-LI in plasma.

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Response of atrial natriuretic peptide to acute saline loading in essential hypertension

AJP Renal Physiology ◽

10.1152/ajprenal.1988.255.6.f1085 ◽

1988 ◽

Vol 255 (6) ◽

pp. F1085-F1090 ◽

Cited By ~ 2

Author(s):

A. Mimran ◽

J. Nussberger ◽

J. Ribstein ◽

B. Waeber ◽

H. R. Brunner

Keyword(s):

Essential Hypertension ◽

Atrial Natriuretic Peptide ◽

Natriuretic Peptide ◽

Volume Expansion ◽

Isotonic Saline ◽

Fractional Excretion ◽

Normal Subjects ◽

Saline Loading ◽

Fractional Excretion Of Sodium ◽

Atrial Natriuretic

To further investigate the mechanism(s) of the exaggerated natriuretic response of hypertensives to volume expansion (VE; 1,800 ml iv isotonic saline over 3 h), the plasma levels of immunoreactive atrial natriuretic peptide (ANP) were measured in 11 normal subjects (NT) and 12 patients with mild essential hypertension (HT). NT and HT groups were similar with respect to age and basal levels of renin, aldosterone and ANP (34.5 +/- 5.5 in NT and 32.5 +/- 6.3 pg/ml in HT, mean +/- SE). In response to VE, ANP increased to the same extent in both groups (a change of 19.3 +/- 5.2 in NT and of 22.2 +/- 7.1 pg/ml in HT) despite the finding of an exaggerated natriuretic response to VE in essential hypertension (36 +/- 3.5 in NT and 54.9 +/- 6.3 nmol/3 h in HT, P less than 0.02). In addition, the fall in hematocrit and serum protein associated with saline infusion was less marked in HT than NT. The change in ANP induced by VE was inversely correlated with the percent fall in hematocrit and the increment in the fractional excretion of sodium in both groups. These observations suggest that ANPs may participate in the control of the renal response to isotonic VE; however they do not support an unequivocal influence of ANP in the exaggerated natriuretic response to VE of patients with essential hypertension.

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Hemodynamic and renal effects following bolus injection of atrial natriuretic peptide (α-hANP) in normal subjects

Acta Endocrinologica ◽

10.1530/acta.0.117s235 ◽

1988 ◽

Vol 117 (4_Suppl) ◽

pp. S235-S236

Author(s):

G. MÜLLER-ESCH ◽

J. POTRATZ ◽

W. KLINGLER ◽

R. GERZER ◽

R. LAWRENZ ◽

...

Keyword(s):

Atrial Natriuretic Peptide ◽

Natriuretic Peptide ◽

Bolus Injection ◽

Normal Subjects ◽

Renal Effects ◽

Atrial Natriuretic

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Radioimmunoassays of Arginine Vasopressin and Atrial Natriuretic Peptide: Application of a Common Protocol for Plasma Extraction Using Sep-Pak C18 Cartridges

Annals of Clinical Biochemistry International Journal of Laboratory Medicine ◽

10.1177/000456329202900606 ◽

1992 ◽

Vol 29 (6) ◽

pp. 652-658 ◽

Cited By ~ 16

Author(s):

M D Penney ◽

D Hampton ◽

D A Oleesky ◽

C Livingstone ◽

E Mulkerrin

Keyword(s):

Standard Deviation ◽

Atrial Natriuretic Peptide ◽

Natriuretic Peptide ◽

Arginine Vasopressin ◽

Normal Subjects ◽

Positive Pressure ◽

Aqueous Methanol ◽

Simultaneous Extraction ◽

Plasma Extraction ◽

Atrial Natriuretic

A rapid vacuum-driven procedure, using pre-treated Sep-Pak C18 cartridges, has been developed for the simultaneous extraction of arginine vasopressin (AVP) and atrial natriuretic peptide (ANP) from plasma. Non-specific interference was removed by fractional elution with an aqueous methanol/trifluoroacetic acid (TFA) mixture. AVP and ANP were coeluted under positive pressure with a methanol/TFA mixture and the eluates air-dried before measurement using separate radioimmunoassays. Assay ranges for AVP and ANP were 0·12–29·5 pmol/L and 0·65–162 pmol/L, respectively, with mean recoveries (standard deviation in parentheses) for AVP of 96·4% (5·5%) at a level of 11·8 pmol/L and for ANP of 94·8% (5·9%) at a level of 32·4 pmol/L. The extraction and assay procedures were validated by observing the changes in plasma AVP and ANP concentrations in normal subjects at different stages of hydration and in elderly patients during treatment for congestive cardiac failure.

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Immunoradiometric assay of atrial natriuretic peptide in unextracted plasma

Clinical Chemistry ◽

10.1093/clinchem/36.6.855 ◽

1990 ◽

Vol 36 (6) ◽

pp. 855-859 ◽

Cited By ~ 7

Author(s):

J E Tattersall ◽

A Dawnay ◽

C McLean ◽

W R Cattell

Keyword(s):

Atrial Natriuretic Peptide ◽

Natriuretic Peptide ◽

Normal Subjects ◽

Disulfide Bridge ◽

Immunoradiometric Assay ◽

Assay Sensitivity ◽

Standard Curve ◽

Hook Effect ◽

C Terminus ◽

Atrial Natriuretic

Abstract We have developed and validated a two-site liquid-phase immunoradiometric assay (IRMA) of atrial natriuretic peptide (ANP) in unextracted human plasma. Both radiolabeled rabbit anti-ANP IgG and polyclonal mouse anti-ANP must bind to ANP for detection, and the assay is specific for peptides with both an intact C-terminus and a disulfide bridge. The assay sensitivity (detection limit) is 0.96 pmol/L, and the working range is 2.3-300 pmol/L, with the hook effect occurring above 500 pmol/L. Results for diluted plasma from normal subjects and from patients with renal failure paralleled the standard curve; analytical recovery of ANP added to such samples averaged 94%. The between- and within-assay CVs at 8 pmol/L were 10% and 5%, respectively. The assay is sufficiently sensitive and precise to detect the postural change in ANP concentrations in normal subjects.

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Atrial Natriuretic Peptide and Renal Disease

Nephrology Dialysis Transplantation ◽

10.1093/ndt/4.12.1008 ◽

1989 ◽

Keyword(s):

Atrial Natriuretic Peptide ◽

Renal Disease ◽

Natriuretic Peptide ◽

Atrial Natriuretic

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Effect of atrial natriuretic peptide on catecholamine release from human pheochromocytoma

Acta Endocrinologica ◽

10.1530/acta.0.1200107 ◽

1989 ◽

Vol 120 (1) ◽

pp. 107-112 ◽

Cited By ~ 5

Author(s):

Mitsuaki Nakamaru ◽

Toshio Ogihara ◽

Hiroshi Saito ◽

Hiromi Rakugi ◽

Kiyoko Hashizume ◽

...

Keyword(s):

Atrial Natriuretic Peptide ◽

Natriuretic Peptide ◽

Direct Action ◽

Plasma Catecholamines ◽

Normal Subjects ◽

Catecholamine Release ◽

Plasma Norepinephrine ◽

Atrial Natriuretic

Abstract. The effect of synthetic alpha human atrial natriuretic peptide on catecholamine release from human pheochromocytomas was studied both in vivo and in vitro. Iv infusion of atrial natriuretic peptide at a rate of 0.1 μg ·kg−1·min−1 for 60 min into two normotensive patients with pheochromocytoma caused a small decrease in the mean blood pressure, increase in the heart rate, and marked increase in the plasma level of norepinephrine (2.08 to 6.83 nmol/l, and 1.15 to 2.83 nmol/l, respectively) compared with 0.60 ± 0.10 to 1.19 ± 0.20 nmol/l in normal subjects. Treatment with atrial natriuretic peptide also increased the plasma epinephrine level from 0.34 to 1.27 nmol/l, and from 0.67 to 0.79 nmol/l in the patients with pheochromocytoma, but not in the normal subjects (0.05 ± 0.01 to 0.05 ± 0.01 nmol/l). After removal of the tumour, the responses of the plasma norepinephrine and epinephrine to atrial natriuretic peptide infusion were normalized. There was no significant effect of 10−8 to 10−5 mol/l atrial natriuretic peptide on the basal release of catecholamines from isolated superfused pheochromocytoma tissue. Atrial natriuretic peptide (10−7 mol/l) did not affect the increase in catecholamine release induced by glucagon (10−5 mol/l). These results suggest that the exaggerated responses of plasma catecholamines to atrial natriuretic peptide in patients with pheochromocytoma may be due to a washout effect resulting from change in blood flow in the vessels feeding the tumour rather than increased sympathetic nerve activity induced by hypotension and hypovolemia. The results also suggest that atrial natriuretic peptide dose not have any direct action on pheochromocytoma tissue causing catecholamine release.

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Atrial Natriuretic Peptide in Kindney of Renal Disease Patients and Healthy Persons.

Endocrinologia Japonica ◽

10.1507/endocrj1954.35.523 ◽

1988 ◽

Vol 35 (4) ◽

pp. 523-529 ◽

Cited By ~ 1

Author(s):

FUMIAKI MARUMO ◽

HISATO SAKAMOTO ◽

NAOKI UMETANI ◽

MICHIHITO OKUBO

Keyword(s):

Atrial Natriuretic Peptide ◽

Renal Disease ◽

Natriuretic Peptide ◽

Healthy Persons ◽

Atrial Natriuretic

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The plasma release of atrial natriuretic peptide in man

Clinical Science ◽

10.1042/cs0710151 ◽

1986 ◽

Vol 71 (2) ◽

pp. 151-155 ◽

Cited By ~ 110

Author(s):

J. V. Anderson ◽

J. Donckier ◽

W. J. McKenna ◽

S. R. Bloom

Keyword(s):

Atrial Natriuretic Peptide ◽

Natriuretic Peptide ◽

Pressure Rise ◽

Plasma Osmolality ◽

Normal Subjects ◽

Solution Saline ◽

The Mean ◽

Maximal Rise ◽

Important Stimulus ◽

Atrial Natriuretic

1. We studied plasma atrial natriuretic peptide (ANP) concentrations in seven normal subjects after the acute intravenous infusion of sodium chloride/potassium chloride solution (saline). 2. Three separate infusions of 6, 12 and 18 ml of saline/kg body weight each significantly increased the circulating concentration of ANP without changes of plasma osmolality or electrolyte concentrations. 3. The mean maximal rise of the plasma ANP concentration after the three saline infusions was significantly correlated (r = 0.74, P < 0.001) with, but occurred 10–30 min later than, the maximal atrial pressure rise. 4. These observations are in accord with the hypotheses that: (a) ANP is a circulating natriuretic factor; (b) atrial distension is an important stimulus to ANP release in man.

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