Serum levels of large tenascin-C variants, matrix metalloproteinase-9, and tissue inhibitors of matrix metalloproteinases in concentric versus eccentric left ventricular hypertrophy

The study objective was to investigate serum concentrations of matrix metalloproteinase-9 (MMP-9) and it inhibitor (TIMP-1) in patients with aortic stenosis and mitral or aortic regurgitation. It was revealed that serum MMP-9 and TIMP-1 levels correlated with type of left ventricular hypertrophy. In patient with mitral or aortic regurgitation (volume load) MMP/TIMP ratio was increased due to prevalence of MMP-9. Increased serum ММР-9/TIMP-1 index was related to enlargement of left ventricular end-diastolic volume. The greatest disbalance in matrix metalloproteinase system was observed in patients with inherent connective tissue disease. In patients with pressure load hypertrophy (aortic stenosis) serum MMP blockade prevalence was observed, which promoted heart fibrosis development. Furthermore, increased serum TIMP-1 level was associated with decreased left ventricular injection fraction in patients with aortic stenosis.

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Preanalytical pitfalls of blood sampling to measure true circulating matrix metalloproteinase 9 and tissue inhibitors of matrix metalloproteinases

Clinica Chimica Acta ◽

10.1016/j.cca.2006.03.001 ◽

2006 ◽

Vol 373 (1-2) ◽

pp. 180-181 ◽

Cited By ~ 10

Author(s):

Klaus Jung ◽

Raquel F. Gerlach ◽

Jose E. Tanus-Santos

Keyword(s):

Matrix Metalloproteinases ◽

Matrix Metalloproteinase ◽

Matrix Metalloproteinase 9 ◽

Blood Sampling ◽

Tissue Inhibitors ◽

Metalloproteinase 9

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Recombinant Matrix Metalloproteinase-9 Induces Ventricular Remodeling By Up-Regulating Angiotensin II

American Journal of Hypertension ◽

10.1093/ajh/hpz189 ◽

2020 ◽

Vol 33 (2) ◽

pp. 205-206

Author(s):

Lu-yuan Luo ◽

Si-qi Gu ◽

Wan-xing Zhou ◽

Li-zhi Chen ◽

Xin-chao You ◽

...

Keyword(s):

Angiotensin Ii ◽

Matrix Metalloproteinase ◽

Cardiac Function ◽

Ventricular Remodeling ◽

Serum Levels ◽

Left Ventricular ◽

Matrix Metalloproteinase 9 ◽

Ang Ii ◽

Protein Levels ◽

Metalloproteinase 9

Abstract Objective To study the effect of matrix metalloproteinase-9 (MMP-9) on ventricular remodeling and involvement of angiotensin II (Ang II). Methods Thirty-six adult male Wistar rats were randomly divided into control (n = 12), MMP-9 (n = 12), and MMP-9 + olmesartan (n = 12) groups. Recombinant MMP-9 (2.1 ng/g) was injected intraperitoneally twice a week. Olmesartan (3 mg/kg) was given by oral gavage once daily. Animals were treated for 4 or 8 weeks. Cardiac function was assessed by echocardiography followed by histological analysis. The messenger RNA (mRNA) and protein levels of MMP-9 in myocardial tissues were analyzed by reverse transcription polymerase chain reaction and Western blotting, respectively. Serum levels of Ang I, II, angiotensin converting enzyme (ACE) and MMP-9 were determined by enzyme-linked immunosorbent assay (ELISA). Results MMP9 administration for 4 or 8 weeks increased mRNA and protein levels of MMP-9 in myocardial tissues, serum levels of MMP-9, Ang I, II and ACE, and left ventricular mass index (LVMI) but decreased collagen volume fraction (CVF) compared with the control group (all P < 0.05). Cardiomyocyte cell size was significantly enlarged and disorganized with cytoplasmic lysis and necrosis in the MMP-9 group. After olmesartan treatment, myocardial MMP-9 mRNA and protein levels, serum levels of MMP-9 and Ang II, and LVMI were lower, and CVF was higher with significant improvement in myocardial morphology and cardiac function (all P<0.05). Conclusion Recombinant MMP-9 treatment induces ventricular remodeling and cardiac dysfunction by up-regulating Ang II.

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High Serum Levels of Matrix Metalloproteinase-9 and Matrix Metalloproteinase-1 Are Associated With Rapid Progression in Patients With Metastatic Melanoma

Yearbook of Dermatology and Dermatologic Surgery ◽

10.1016/s0093-3619(08)70290-9 ◽

2006 ◽

Vol 2006 ◽

pp. 352-353

Author(s):

B.H. Thiers

Keyword(s):

Matrix Metalloproteinase ◽

Metastatic Melanoma ◽

Serum Levels ◽

High Serum ◽

Matrix Metalloproteinase 9 ◽

Rapid Progression ◽

Matrix Metalloproteinase 1 ◽

Metalloproteinase 9

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The role of matrix metalloproteinase 9 and its inhibitors in scarring processes following surgery for primary open-angle glaucoma

ZHurnal «Patologicheskaia fiziologiia i eksperimental`naia terapiia» ◽

10.25557/0031-2991.2019.04.72-80 ◽

2019 ◽

pp. 72-80

Author(s):

Е.В. Маркелова ◽

О.В. Овчинникова ◽

А.С. Хохлова ◽

Л.П. Догадова ◽

А.В. Костюшко ◽

...

Keyword(s):

Matrix Metalloproteinase ◽

Postoperative Period ◽

Open Angle Glaucoma ◽

Matrix Metalloproteinase 9 ◽

Tissue Inhibitors Of Metalloproteinases ◽

Open Angle ◽

Tissue Inhibitors ◽

Surgery Outcome ◽

Metalloproteinase 9

Оперативное вмешательство - один из основных методов лечения глаукомы. Однако развитие избыточного рубцевания созданных путей оттока определяет результат хирургического лечения в отдаленные сроки. Процессы рубцевания на данный момент недостаточно изучены. Цель исследования - оценка роли матриксной металлопротеиназы-9, ее ингибиторов в процессах рубцевания у больных с первичной открытоугольной глаукомой после оперативного лечения. Методика. Для выявления возможных маркеров избыточного рубцевания методом твердофазного иммуноферментного анализа определяли содержание матриксных металлопротеиназ-9, тканевых ингибиторов металлопротеиназ 2 и -3 в слезной жидкости у 37 пациентов с активной стадией первичной остроугольной глаукомы в динамике послеоперационного периода. Средний возраст пациентов составил 52,8 лет. В зависимости от исхода оперативного вмешательства все пациенты были разделены на 2 группы - с благоприятным исходом (без избыточного рубцевания) и с неблагоприятным исходом (с избыточным рубцеванием) на месте сформированных дополнительных путей оттока внутриглазной жидкости в послеоперационном периоде. Группа контроля включала 20 человек в возрасте от 50 до 66 лет без сопутствующей офтальмологической и соматической патологии в стадии обострения. Результаты. В динамике показано изменение концентрации матриксной металлопротеиназы-9 и ее ингибиторов в послеоперационном периоде. Анализ данных свидетельствует об обратной зависимости уровня матриксной металлопротеиназы-9 и тканевых ингибиторов металлопротеиназы 2 и 3 типов с исходом операции - чем выше концентрация металлопротеиназы-9 и ниже концентрация тканевых ингибиторов металлопротеиназ 2, -3 в слезной жидкости, тем выше вероятность неблагоприятного исхода в виде рубцевания сформированных дополнительных путей оттока внутриглазной жидкости в послеоперационном периоде. Заключение. Мониторинг уровня металлопротеиназ и их тканевых ингибиторов после проведения хирургического лечения пациентов с первичной открытоугольной глаукомой позволяет прогнозировать раннее рубцевание, дает возможность разработки новых методов лечения как в раннем, так и в позднем послеоперационном периоде. Surgery is one of the major treatments for glaucoma; however excessive scarring of created outflow patways affects the long-term outcome. At the present time, scarring processes are not sufficiently studied. Aim. To evaluate the role of matrix metalloproteinase 9 and its inhibitors in scarring after surgical treatment of open-angle glaucoma. Methods. Concentrations of matrix metalloproteinase 9 and tissue inhibitors of metalloproteinases 2 and 3 were measured in tear fluid of 37 patients (mean age, 52.8) with active primary open-angle glaucoma in dynamics during the postoperative period to identify possible markers of excessive scarring. Based on the surgery outcome, all patients were divided into two groups, with a favorable outcome (without excessive scarring) and an unfavorable outcome (with excessive scarring) in the created additional outflow pathways for the intraocular fluid in the postoperative period. The control group included 20 subjects aged 50-66 without eye disease or somatic disease at exacerbation stage. Results. Analysis of changes in concentrations of matrix metalloproteinase 9 and its inhibitors in the postoperative period showed their inverse relationship with the surgery outcome. The higher was the metalloproteinase 9 level and the lower the level of tissue inhibitors of metalloproteinases 2 and 3 the higher was the probability of unfavorable outcome evident as excessive scarring of the formed additional pathways for tear fluid outflow in the postoperative period. Conclusion. Postoperative monitoring of metalloproteinases and their tissue inhibitors allows to predict early scarring and to develop new treatments both in early and late postoperative periods.

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