scholarly journals Integrated Genetic Map of Anopheles gambiae: Use of RAPD Polymorphisms for Genetic, Cytogenetic and STS Landmarks

Genetics ◽  
1996 ◽  
Vol 143 (2) ◽  
pp. 953-960
Author(s):  
George Dimopoulos ◽  
Liangbiao Zheng ◽  
Vasantha Kumar ◽  
Alessandra della Torre ◽  
Fotis C Kafatos ◽  
...  
Keyword(s):  
Anopheles Gambiae ◽  
Polytene Chromosomes ◽  
Sequence Tagged Sites ◽  
Entry Points ◽  
Vector Mosquito ◽  
Mosquito Anopheles Gambiae ◽  
Generating Sequence ◽  
Integrated Genetic Map ◽  
Gambiae Genome

Abstract Randomly amplified polymorphic DNA (RAPD) markers have been integrated in the genetic and cytogenetic maps of the malaria vector mosquito, Anopheles gambiae. Fifteen of these markers were mapped by recombination, relative to microsatellite markers that had been mapped previously. Thirty-four gel-purified RAPD bands were cloned and sequenced, generating sequence tagged sites (STSs) that can be used as entry points to the A. gambiae genome. Thirty one of these STSs were localized on nurse cell polytene chromosomes through their unique hybridization signal in in situ hybridization experiments. Five STSs map close to the breakpoints of polymorphic inversions, which are notable features of the Anopheles genome. The usefulness and limitations of this integrated mosquito map are discussed.

scholarly journals An Integrated Genetic Map of the African Human Malaria Vector Mosquito, Anopheles gambiae

Genetics ◽  
1996 ◽  
Vol 143 (2) ◽  
pp. 941-952 ◽  
Author(s):  
Liangbiao Zheng ◽  
Mark Q Benedict ◽  
Anton J Cornel ◽  
Frank H Collins ◽  
Fotis C Kafatos
Keyword(s):  
Microsatellite Markers ◽  
Anopheles Gambiae ◽  
Genetic Map ◽  
Malaria Vector ◽  
Average Distance ◽  
Polytene Chromosomes ◽  
Human Malaria ◽  
The Third ◽  
Vector Mosquito ◽  
Mosquito Anopheles Gambiae

Abstract We present a genetic map based on microsatellite polymorphisms for the African human malaria vector, Anopheles gambiae. Polymorphisms in laboratory strains were detected for 89% of the tested microsatellite markers. Genotyping was performed for individual mosquitoes from 13 backcross families that included 679 progeny. Three linkage groups were identified, corresponding to the three chromosomes. We added 22 new markers to the existing X chromosome map, for a total of 46 microsatellite markers spanning a distance of 48.9 cM. The second chromosome has 57 and the third 28 microsatellite markers spanning a distance of 72.4 and 93.7 cM, respectively. The overall average distance between markers is 1.6 cM (or 1.1, 1.2, and 3.2 cM for the X, second, and third chromosomes, respectively). In addition to the 131 microsatellite markers, the current map also includes a biochemical selectable marker, Dieldrin resistance (Dl), on the second chromosome and five visible markers, pink-eye (p) and white (w) on the X, collarless (c) and lunate (lu) on the second, and red-eye (r) on the third. The cytogenetic locations on the nurse cell polytene chromosomes have been determined for 47 markers, making this map an integrated tool for cytogenetic, genetic, and molecular analysis.

scholarly journals Antennal-Expressed Ammonium Transporters in the Malaria Vector Mosquito Anopheles gambiae

PLoS ONE ◽  
2014 ◽  
Vol 9 (10) ◽  
pp. e111858 ◽  
Author(s):  
R. Jason Pitts ◽  
Stephen L. Derryberry ◽  
Fadi E. Pulous ◽  
Laurence J. Zwiebel
Keyword(s):  
Anopheles Gambiae ◽  
Malaria Vector ◽  
Ammonium Transporters ◽  
Vector Mosquito ◽  
Mosquito Anopheles Gambiae

Analysis of a lysozyme gene from the malaria vector mosquito, Anopheles gambiae

Gene ◽  
1996 ◽  
Vol 174 (2) ◽  
pp. 239-244 ◽  
Author(s):  
Daiwu Kang ◽  
Patricia Romans ◽  
Jong-Youn Lee
Keyword(s):  
Anopheles Gambiae ◽  
Malaria Vector ◽  
Lysozyme Gene ◽  
Vector Mosquito ◽  
Mosquito Anopheles Gambiae

The defensin peptide of the malaria vector mosquito Anopheles gambiae: antimicrobial activities and expression in adult mosquitoes

2001 ◽  
Vol 31 (3) ◽  
pp. 241-248 ◽  
Author(s):  
Jacopo Vizioli ◽  
Adam M. Richman ◽  
Sandrine Uttenweiler-Joseph ◽  
Claudia Blass ◽  
Philippe Bulet
Keyword(s):  
Anopheles Gambiae ◽  
Malaria Vector ◽  
Antimicrobial Activities ◽  
Vector Mosquito ◽  
Mosquito Anopheles Gambiae
Sign in / Sign up

Export Citation Format

Share Document