scholarly journals Identification and Physical Localization of Useful Genes and Markers to a Major Gene-Rich Region on Wheat Group1SChromosomes

Genetics ◽  
2001 ◽  
Vol 157 (4) ◽  
pp. 1735-1747 ◽  
Author(s):  
Devinder Sandhu ◽  
Julie A Champoux ◽  
Svetlana N Bondareva ◽  
Kulvinder S Gill
Keyword(s):  
Dna Analysis ◽  
Major Gene ◽  
Rflp Markers ◽  
Rich Region ◽  
Wheat Group ◽  
Marker Loci ◽  
Group 1 Chromosomes ◽  
Gene Rich Region ◽  
Homeologous Group ◽  
Group 1

AbstractThe short arm of Triticeae homeologous group 1 chromosomes is known to contain many agronomically important genes. The objectives of this study were to physically localize gene-containing regions of the group 1 short arm, enrich these regions with markers, and study the distribution of genes and recombination. We focused on the major gene-rich region (“1S0.8 region”) and identified 75 useful genes along with 93 RFLP markers by comparing 35 different maps of Poaceae species. The RFLP markers were tested by gel blot DNA analysis of wheat group 1 nullisomic-tetrasomic lines, ditelosomic lines, and four single-break deletion lines for chromosome arm 1BS. Seventy-three of the 93 markers mapped to group 1 and detected 91 loci on chromosome 1B. Fifty-one of these markers mapped to two major gene-rich regions physically encompassing 14% of the short arm. Forty-one marker loci mapped to the 1S0.8 region and 10 to 1S0.5 region. Two cDNA markers mapped in the centromeric region and the remaining 24 loci were on the long arm. About 82% of short arm recombination was observed in the 1S0.8 region and 17% in the 1S0.5 region. Less than 1% recombination was observed for the remaining 85% of the physical arm length.

Interspecific nuclear–cytoplasmic compatibility controlled by genes on group 1 chromosomes in durum wheat

Genome ◽  
1995 ◽  
Vol 38 (4) ◽  
pp. 803-808 ◽  
Author(s):  
J. A. Anderson ◽  
S. S. Maan
Keyword(s):  
Dna Markers ◽  
Male Fertility ◽  
Spontaneous Mutation ◽  
Nuclear Genome ◽  
Rflp Markers ◽  
Seedling Vigor ◽  
Group 1 Chromosomes ◽  
Two Populations ◽  
Aneuploid Analysis ◽  
Group 1

Triticum longissimum cytoplasm is incompatible with the T. turgidum nuclear genome. Two nuclear genes, scs and Vi, derived from the nuclear genome of T. timopheevii and by a spontaneous mutation, respectively, restore nuclear–cytoplasmic compatibility, normal plant vigor, and male fertility in these alloplasmic genotypes. The objectives of this study were (i) to determine the chromosomal locations of scs and Vi; (ii) to identify DNA markers for scs and Vi; and (iii) to determine the interactions involving the dosage of scs and Vi. Two populations segregating for scs and Vi were produced and scored for seedling vigor (indicating presence of scs) and degree of self-fertility (indicating presence of Vi). Four RFLP markers were mapped near scs. Aneuploid analysis revealed that these markers, and hence the scs gene, are located on the long arm of chromosome 1A. Four RFLP markers were mapped near Vi on 1BS. Results indicated that other factors may be inhibiting the expression of Vi. We determined the dosage of scs and Vi in both populations with the aid of the linked RFLP markers. Individuals with two versus one dose of scs had reduced self-fertility, while individuals with two versus one dose of Vi had similar self-fertility.Key words: scs, Vi, Triticum, nucleocytoplasmic compatibility, RFLP.

The molecular identification of the midget chromosome from the rye genome

Genome ◽  
1994 ◽  
Vol 37 (6) ◽  
pp. 1056-1061 ◽  
Author(s):  
M. G. Francki ◽  
P. Langridge
Keyword(s):  
Genomic Library ◽  
Nuclear Genome ◽  
Repeat Sequence ◽  
Proximal Region ◽  
Rflp Markers ◽  
Repeated Dna Sequence ◽  
Chromosome 1R ◽  
Group 1 Chromosomes ◽  
Group 1

The diminutive "midget" chromosome is found in plants containing a wheat nuclear genome with a substituted rye cytoplasm. This cytoplasmic substituted line arose during successive backcrossing of a wheat/rye amphiploid to wheat as the recurrent male parent. Southern and in situ hybridization with a dispersed repeat sequence specific for rye, R173, indicates that the midget chromosome originates from within the rye genome. Various DNA markers previously mapped to group 1 chromosomes of wheat and barley were used to trace the origin of the midget chromosome from within the rye genome. Ten short arm and 36 long arm probes were used and one marker was identified, which hybridizes to the midget chromosome and maps to the proximal region of the long arm of chromosome 1R. An additional marker was generated from a genomic library of the line containing the midget chromosome. This also maps to the long arm of 1R. The results indicate that the midget chromosome contains a small segment of the long arm of chromosome 1R.Key words: midget chromosome, RFLP markers, chromosome 1R, repeated DNA sequence.

Chromosomal localization of seven HSA3q13→q23 NotI linking clones on chicken microchromosomes: orthology of GGA14 and GGA15 to a gene-rich region of HSA3

2005 ◽  
Vol 111 (2) ◽  
pp. 128-133 ◽  
Author(s):  
A.A. Sazanov ◽  
A.L. Sazanova ◽  
V.A. Stekol’nikova ◽  
A.A. Kozyreva ◽  
M.N. Romanov ◽  
...  
Keyword(s):  
Chromosomal Localization ◽  
Rich Region ◽  
Gene Rich Region

scholarly journals The highly recombinogenic bz locus lies in an unusually gene-rich region of the maize genome

2001 ◽  
Vol 98 (15) ◽  
pp. 8903-8908 ◽  
Author(s):  
H. Fu ◽  
W. Park ◽  
X. Yan ◽  
Z. Zheng ◽  
B. Shen ◽  
...  
Keyword(s):  
Maize Genome ◽  
Rich Region ◽  
Gene Rich Region

Rps 8 Maps to a Resistance Gene Rich Region on Soybean Molecular Linkage Group F

Crop Science ◽  
2006 ◽  
Vol 46 (1) ◽  
pp. 168-173 ◽  
Author(s):  
Stuart G. Gordon ◽  
Steven K. St. Martin ◽  
Anne E. Dorrance
Keyword(s):  
Linkage Group ◽  
Resistance Gene ◽  
Rich Region ◽  
Molecular Linkage Group ◽  
Gene Rich Region

A family of endosperm globulins encoded by genes located in group 1 chromosomes of wheat and related species

1988 ◽  
Vol 214 (3) ◽  
pp. 541-546 ◽  
Author(s):  
Luis Gomez ◽  
Rosa Sanchez-Monge ◽  
Gabriel Salcedo
Keyword(s):  
Related Species ◽  
Group 1 Chromosomes ◽  
Group 1

Transcript Mapping of the Human Chromosome 11q12–q13.1 Gene-Rich Region Identifies Several Newly Described Conserved Genes

Genomics ◽  
1998 ◽  
Vol 49 (3) ◽  
pp. 419-429 ◽  
Author(s):  
Paul R. Cooper ◽  
Norma J. Nowak ◽  
Michael J. Higgins ◽  
Deanna M. Church ◽  
Thomas B. Shows
Keyword(s):  
Human Chromosome ◽  
Transcript Mapping ◽  
Rich Region ◽  
Conserved Genes ◽  
Gene Rich Region

Structure–function analysis of the barley genome: the gene-rich region of chromosome 2HL

2008 ◽  
Vol 9 (1) ◽  
pp. 67-79 ◽  
Author(s):  
Andrew Chen ◽  
Anita Brûlé-Babel ◽  
Ute Baumann ◽  
Nicholas C. Collins
Keyword(s):  
Structure Function ◽  
Function Analysis ◽  
Rich Region ◽  
Barley Genome ◽  
Gene Rich Region

scholarly journals Molecular-genetic maps for group 1 chromosomes of Triticeae species and their relation to chromosomes in rice and oat

Genome ◽  
1995 ◽  
Vol 38 (1) ◽  
pp. 45-59 ◽  
Author(s):  
A. E. Van Deynze ◽  
J. Dubcovsky ◽  
K. S. Gill ◽  
J. C. Nelson ◽  
M. E. Sorrells ◽  
...  
Keyword(s):  
Dna Markers ◽  
Molecular Genetic ◽  
Genetic Maps ◽  
Chromosome 1 ◽  
Linkage Maps ◽  
Consensus Map ◽  
Triticeae Species ◽  
Group 1 Chromosomes ◽  
Homoeologous Chromosomes ◽  
Group 1

Group 1 chromosomes of the Triticeae tribe have been studied extensively because many important genes have been assigned to them. In this paper, chromosome 1 linkage maps of Triticum aestivum, T. tauschii, and T. monococcum are compared with existing barley and rye maps to develop a consensus map for Triticeae species and thus facilitate the mapping of agronomic genes in this tribe. The consensus map that was developed consists of 14 agronomically important genes, 17 DNA markers that were derived from known-function clones, and 76 DNA markers derived from anonymous clones. There are 12 inconsistencies in the order of markers among seven wheat, four barley, and two rye maps. A comparison of the Triticeae group 1 chromosome consensus map with linkage maps of homoeologous chromosomes in rice indicates that the linkage maps for the long arm and the proximal portion of the short arm of group 1 chromosomes are conserved among these species. Similarly, gene order is conserved between Triticeae chromosome 1 and its homoeologous chromosome in oat. The location of the centromere in rice and oat chromosomes is estimated from its position in homoeologous group 1 chromosomes of Triticeae.Key words: Triticeae, RFLP, consensus, comparative.

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