Rps 8 Maps to a Resistance Gene Rich Region on Soybean Molecular Linkage Group F

Crop Science ◽  
2006 ◽  
Vol 46 (1) ◽  
pp. 168-173 ◽  
Author(s):  
Stuart G. Gordon ◽  
Steven K. St. Martin ◽  
Anne E. Dorrance
Genome ◽  
1999 ◽  
Vol 42 (3) ◽  
pp. 453-456 ◽  
Author(s):  
Yun Hai Lu ◽  
Geneviève Gagne ◽  
Bruno Grezes-Besset ◽  
Philippe Blanchard

Genome ◽  
1999 ◽  
Vol 42 (3) ◽  
pp. 453-456 ◽  
Author(s):  
Yun Hai Lu ◽  
Geneviève Gagne ◽  
Bruno Grezes-Besset ◽  
Philippe Blanchard

A linkage group containing the Or5 gene conferring resistance to Orobanche cumana race E, as well as 5 SCAR markers and 1 RAPD marker has been recently identified in sunflower. A SCAR marker RTS05, mapped 5.6 cM proximal to the Or5 locus, was analysed in an F2 population for which the segregation data of 80 RFLP markers (GIE cartisol - Phase II, France) were available. An association was found between the SCAR marker RTS05 and an RFLP marker S009 (32.1 cM, LOD = 4.7) that had been mapped to the linkage group 17 of the GIE Cartisol RFLP map. Another RFLP marker S010, tightly linked to S009 (0.0 cM) in the same linkage group, was screened in the F2 population that had been previously used for the Or5 linkage map identification. S010 was found to be significantly linked to all 5 SCAR markers as well as to the single RAPD marker with a LOD > 3.0 in each case. This RFLP marker was mapped between two SCAR markers and was situated at 35.1 cM from the resistance gene with a LOD = 2.7. These results showed that the Or5 linkage group could be integrated with the linkage group 17 of the GIE Cartisol RFLP map.Key words: Helianthus, Orobanche, RFLP, SCAR, linkage map.


Genome ◽  
2006 ◽  
Vol 49 (9) ◽  
pp. 1140-1148 ◽  
Author(s):  
C. Bass ◽  
R. Hendley ◽  
M.J. Adams ◽  
K.E. Hammond-Kosack ◽  
K. Kanyuka

A mosaic disease caused by Soil-borne cereal mosaic virus (SBCMV) is becoming increasingly important, particularly in winter wheat in Europe. As there are currently no effective cultural practices or practical environmentally friendly chemicals for disease control, host plant resistance is an important objective in breeding programs. However, development of resistant cultivars is slow owing to difficulties in germplasm screening for resistance. Therefore, there is a need to identify molecular markers linked to SBCMV-resistance gene(s), so that quick and accurate laboratory-based marker-assisted selection rather than prolonged field-based screens for resistance can be used in developing resistant cultivars. We previously demonstrated that resistance to SBCMV in Triticum aestivum ‘Cadenza’ is controlled by a single locus. In this work, we used AFLP and microsatellite technology to map this resistance locus, with the proposed name Sbm1, to the distal end of chromosome 5DL. Interestingly, several expressed disease-resistance gene analogues also map to this gene-rich region on 5DL. Closely linked (~17 cM interval) markers, BARC110 and WMC765, RRES01 and BARC144, that flank Sbm1 will be very useful in breeding for selection of germplasm carrying Sbm1.


Genetics ◽  
2001 ◽  
Vol 157 (4) ◽  
pp. 1735-1747 ◽  
Author(s):  
Devinder Sandhu ◽  
Julie A Champoux ◽  
Svetlana N Bondareva ◽  
Kulvinder S Gill

AbstractThe short arm of Triticeae homeologous group 1 chromosomes is known to contain many agronomically important genes. The objectives of this study were to physically localize gene-containing regions of the group 1 short arm, enrich these regions with markers, and study the distribution of genes and recombination. We focused on the major gene-rich region (“1S0.8 region”) and identified 75 useful genes along with 93 RFLP markers by comparing 35 different maps of Poaceae species. The RFLP markers were tested by gel blot DNA analysis of wheat group 1 nullisomic-tetrasomic lines, ditelosomic lines, and four single-break deletion lines for chromosome arm 1BS. Seventy-three of the 93 markers mapped to group 1 and detected 91 loci on chromosome 1B. Fifty-one of these markers mapped to two major gene-rich regions physically encompassing 14% of the short arm. Forty-one marker loci mapped to the 1S0.8 region and 10 to 1S0.5 region. Two cDNA markers mapped in the centromeric region and the remaining 24 loci were on the long arm. About 82% of short arm recombination was observed in the 1S0.8 region and 17% in the 1S0.5 region. Less than 1% recombination was observed for the remaining 85% of the physical arm length.


2005 ◽  
Vol 111 (2) ◽  
pp. 128-133 ◽  
Author(s):  
A.A. Sazanov ◽  
A.L. Sazanova ◽  
V.A. Stekol’nikova ◽  
A.A. Kozyreva ◽  
M.N. Romanov ◽  
...  

2001 ◽  
Vol 98 (15) ◽  
pp. 8903-8908 ◽  
Author(s):  
H. Fu ◽  
W. Park ◽  
X. Yan ◽  
Z. Zheng ◽  
B. Shen ◽  
...  

Genome ◽  
1997 ◽  
Vol 40 (5) ◽  
pp. 659-665 ◽  
Author(s):  
Evans S. Lagudah ◽  
Odile Moullet ◽  
Rudi Appels

The Cre3 gene confers a high level of resistance to the root endoparasitic nematode Heterodera avenae in wheat. A DNA marker cosegregating with H. avenae resistance was used as an entry point for map-based cloning of a disease resistance gene family at the Cre3 locus. Two related gene sequences have been analysed at the Cre3 locus. One, identified as a cDNA clone, encodes a polypeptide with a nucleotide binding site (NBS) and a leucine-rich region; this member of the disease resistance gene family is expressed in roots. A second Cre3 gene sequence, cloned as genomic DNA, appears to be a pseudogene, with a frame shift caused by a deletion event. These two genes, related to members of the cytoplasmic NBS – leucine rich repeat class of plant disease resistance genes were physically mapped to the distal 0.06 fragment of the long arm of wheat chromosome 2D and cosegregated with nematode resistance.Key words: cereal cyst nematode, disease resistance genes, nucleotide-binding site, leucine-rich repeat.


Genomics ◽  
1998 ◽  
Vol 49 (3) ◽  
pp. 419-429 ◽  
Author(s):  
Paul R. Cooper ◽  
Norma J. Nowak ◽  
Michael J. Higgins ◽  
Deanna M. Church ◽  
Thomas B. Shows

Genome ◽  
2008 ◽  
Vol 51 (1) ◽  
pp. 64-72 ◽  
Author(s):  
Fengqun Yu ◽  
Derek J. Lydiate ◽  
S. Roger Rimmer

The spectrum of resistance to isolates of Leptosphaeria maculans and the map location of a new blackleg resistance gene found in the canola cultivar Brassica napus ‘Surpass 400’ are described. Two blackleg resistance genes, LepR1 and LepR2, from B. rapa subsp. sylvestris and introgressed in B. napus were identified previously. ‘Surpass 400’ also has blackleg resistance introgressed from B. rapa subsp. sylvestris. Using 31 diverse isolates of L. maculans, the disease reaction of ‘Surpass 400’ was compared with those of the resistant breeding lines AD9 (which contains LepR1), AD49 (which contains LepR2), and MC1-8 (which contains both LepR1 and LepR2). The disease reaction on ‘Surpass 400’ was different from those observed on AD9 and MC1-8, indicating that ‘Surpass 400’ carries neither LepR1 nor both LepR1 and LepR2 in combination. Disease reactions of ‘Surpass 400’ to most of the isolates tested were indistinguishable from those of AD49, which suggested ‘Surpass 400’ might contain LepR2 or a similar resistance gene. Classical genetic analysis of F1 and BC1 plants showed that a dominant allele conferred resistance to isolates of L. maculans in ‘Surpass 400’. The resistance gene, which mapped to B. napus linkage group N10 in an interval of 2.9 cM flanked by microsatellite markers sR12281a and sN2428Rb and 11.7 cM below LepR2, was designated LepR3. A 9 cM region of the B. napus genome containing LepR3 was found to be syntenic with a segment of Arabidopsis chromosome 5.


2008 ◽  
Vol 9 (1) ◽  
pp. 67-79 ◽  
Author(s):  
Andrew Chen ◽  
Anita Brûlé-Babel ◽  
Ute Baumann ◽  
Nicholas C. Collins

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