O-glycosylation in Spodoptera frugiperda (Sf9) and Trichoplusia ni (Hi-5) insect cell lines is complex and include abundant hexuronic acid (Sf9 and Hi-5) and O-linked phosphocholine (Sf9)

Some insect tissue culture cells may be readily infected with inocula of infectious hemolymph from the respective diseased host species or infectious cell culture supernatants from the respective infected tissue culture cells. We were interested in studying the degree of specificity of several insect cell lines to non-host viruses. Viruses investigated were baculoviruses (BV) isolated from corn earworm, Heliothis zea (Boddie); cabbage looper, Trichoplusia ni (Hübner) ; fall armyworm, Spodoptera frugiperda (J. D. Smith); alfalfa looper, Autographa californica (Speyer); gypsy moth, Porthetria dispar (L.) and cotton bollworm, Heliothis armigera (Hubner) on cell lines obtained from T. ni, H. zea, S. frugiperda.

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Trichoplusia ni cells (High FiveTM) are highly efficient for the production of influenza A virus-like particles: a comparison of two insect cell lines as production platforms for influenza vaccines

Molecular Biotechnology ◽

10.1007/s12033-010-9268-3 ◽

2010 ◽

Vol 45 (3) ◽

pp. 226-234 ◽

Cited By ~ 82

Author(s):

Florian Krammer ◽

Theresa Schinko ◽

Dieter Palmberger ◽

Christopher Tauer ◽

Paul Messner ◽

...

Keyword(s):

Influenza A Virus ◽

Cell Lines ◽

Insect Cell ◽

Trichoplusia Ni ◽

Influenza A ◽

Influenza Vaccines ◽

Virus Like Particles ◽

Highly Efficient ◽

Insect Cell Lines

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The role of host cell physiology in the productivity of the baculovirus-insect cell system: Fluxome analysis of Trichoplusia ni and Spodoptera frugiperda cell lines

Biotechnology and Bioengineering ◽

10.1002/bit.26089 ◽

2016 ◽

Vol 114 (3) ◽

pp. 674-684 ◽

Cited By ~ 7

Author(s):

Francisca Monteiro ◽

Vicente Bernal ◽

Paula M. Alves

Keyword(s):

Host Cell ◽

Cell Lines ◽

Spodoptera Frugiperda ◽

Insect Cell ◽

Trichoplusia Ni ◽

Cell System ◽

Cell Physiology

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A recombinant baculovirus TnGV in Trichoplusia ni larvae using the PIG bombardment method and the CRISPR/Cas9 system

10.1101/2021.08.01.454629 ◽

2021 ◽

Author(s):

Oscar J. Ortiz-Arrazola ◽

Maria Cristina Del Rincon-Castro

Keyword(s):

Cell Lines ◽

Insect Cell ◽

Trichoplusia Ni ◽

High Efficiency ◽

Recombinant Baculovirus ◽

Heterologous Proteins ◽

Reporter Protein ◽

Insect Cell Lines ◽

Specific Sequences

Baculoviruses have been used for the expression of heterologous proteins of biotechnological interest. However, most of these proteins are obtained by homologous co-transfection recombination in cell lines, limiting their use. Recently, the CRISPR/Cas9 system has excelled in its high efficiency in editing specific sequences without the need for insect cell lines. In this work, the CRISPR/Cas9 system was used to edit the genome of Trichopusia ni granulovirus (TnGV) and transformation of insects by the PIG bombardment method. A homologous repair vector (pTnGV101) was designed with regions orf5 and orf7, as well as sgRNA flanking TnGV P10 of this virus. The bombardment transformation was carried out at 175 psi with 40% of infected T. ni larvae, of which 38% expressed the reporter protein EGFP. These results demonstrate that the CRISPR/Cas9 system and PIG bombardment can be used for genetic modification of baculovirus in vivo.

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