Multiple Infections by Cytomegalovirus in Patients with Acquired Immunodeficiency Syndrome: Documentation by Southern Blot Hybridization

High-grade malignant nonHodgkin's lymphomas--five lymphoblastic, three pleomorphic, and two immunoblastic--developed in 10/25 cynomolgus monkeys (Macaca fascicularis) followed for up to 746 d after infection with simian immunodeficiency virus, strain SIVsm. These lymphomas were shown to be associated with an Epstein-Barr (EB)-like cynomolgus B-lymphotropic herpesvirus (CBLV) by electron microscopy, by Southern blot hybridization with probes against human EBV, and by the expression of antigens corresponding to EBV-associated nuclear antigens (EBNAs) involved in human B cells transformation. Southern blot demonstration of immunoglobulin gene rearrangements and homogeneous EBV episomes indicated that all the lymphomas were CBLV-associated monoclonal B cell proliferations. Our findings suggest that these tumors correspond to the EBV-associated malignant lymphomas in acquired immunodeficiency syndrome with respect to clinical, morphological, phenotypic, and genotypic characteristics. The particular susceptibility of SIVsm immunodeficient cynomolgus monkeys for CBLV-associated lymphomagenesis appears therefore a useful model for EBV-associated lymphomas in humans.

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Agreement between premortem and postmortem diagnoses in patients with acquired immunodeficiency syndrome observed at a brazilian teaching hospital

Revista do Instituto de Medicina Tropical de São Paulo ◽

10.1590/s0036-46651997000400007 ◽

1997 ◽

Vol 39 (4) ◽

pp. 217-222 ◽

Cited By ~ 16

Author(s):

Aércio Sebastião BORGES ◽

Marcelo Simão FERREIRA ◽

Sérgio de Andrade NISHIOKA ◽

Marco Túlio Alvarenga SILVESTRE ◽

Arnaldo Moreira SILVA ◽

...

Keyword(s):

Acquired Immunodeficiency Syndrome ◽

Causes Of Death ◽

Single Case ◽

Postmortem Examination ◽

Multiple Infections ◽

Postmortem Diagnosis ◽

Clinical Diagnoses ◽

Acquired Immunodeficiency ◽

Immunodeficiency Syndrome ◽

Postmortem Examinations

Acquired immunodeficiency syndrome (AIDS) is one of the main causes of death in adults worldwide. More commonly than in the general population, in patients with AIDS there is substantial disagreement between causes of death which are clinically suspected and those established by postmortem examination. The findings of 52 postmortem examinations were compared to the premortem (clinical) diagnoses, and there was 46% agreement between them. Fifty two percent of the patients had more than one postmortem diagnosis, and 48% had at least one AIDS-related disease not suspected clinically. Cytomegalovirus infection was the commonest (30.7%) autopsy finding, but not a single case had been suspected premortem. Bacterial infection, tuberculosis, and histoplasmosis were also common, sometimes not previously suspected, postmortem findings. This study shows that multiple infections occur simultaneously in AIDS patients, and that many among them are never suspected before the postmortem examination. These findings suggest that an aggressive investigation of infections and cancers should be done in patients with AIDS, particularly in those who do not respond to therapy of an already recognized condition

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