scholarly journals Liquid Chromatographic Determination of Fenbendazole Residues in Pig Tissues after Treatment with Medicated Feed

1999 ◽  
Vol 82 (5) ◽  
pp. 1007-1016 ◽  
Author(s):  
Bettencourt P S Capece ◽  
Belén Pérez ◽  
Eugeni Castells ◽  
Margarita Arboix ◽  
Carles Cristòfol
Keyword(s):  
High Performance ◽  
Chromatographic Determination ◽  
Farm Animals ◽  
Liquid Liquid Extraction ◽  
Limit Of Quantitation ◽  
Maximum Residue Limits ◽  
Cleanup Procedure ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract Fenbendazole (FBZ) is an anthelmintic widely used in farm animals to treat parasitic infestations, in pigs, it is administered in the food. The aim of this study was to validate an analytical method for the determination of FBZ and its metabolites in pig tissues. This method is based on oxidation of FBZ and its sulfoxide metabolite to the sulfone metabolite (FBZSO2). The limit of quantitation for this method is 20 ng FBZSO2/g for all tissues. The maximum residue limits (MRLs) established for FBZ and its metabolites in pig tissues are 50 ng/g for muscle, fat, and kidney and 500 ng/g for liver. This method is based on a liquid-liquid extraction followed by an oxidation with peracetic acid and a cleanup procedure based on 2 liquid-liquid extractions. Determination is achieved by high- performance liquid chromatography with ultraviolet detection. The present method is adjusted to the MRL established for FBZ and its metabolite residues. The analysis of the residues shows that after 72 h posttreatment, no FBZSO2 was detected in muscle, fat, and kidney and that liver levels were below the MRL.

High Performance Liquid Chromatographic Determination of Fructose, Glucose, and Sucrose in Molasses

1980 ◽  
Vol 63 (3) ◽  
pp. 476-480 ◽  
Author(s):  
Carolyn E Damon ◽  
Bruce C Pettitt
Keyword(s):  
High Performance ◽  
Reducing Sugars ◽  
Internal Standard ◽  
Chromatographic Determination ◽  
High Performance Liquid Chromatographic ◽  
Cleanup Procedure ◽  
Liquid Chromatographic Determination ◽  
Standard Solution ◽  
Liquid Chromatographic

Abstract Fructose, glucose, and sucrose were determined quantitatively in molasses by high performance liquid chromatography (HPLC) using maltose as an internal standard. After a simple cleanup procedure, chromatographic separation required less than 20 min for the 17 molasses samples. HPLC gave lower results for reducing sugars (fructose plus glucose) and for sucrose by an average of 3.9 and 1.2%, respectively, when compared to results obtained by classical methods. The lower values are believed to result from elimination of interfering substances by this method. The values probably reflect more accurately the true composition of the molasses. Repetitive injections of a standard solution indicated a coefficient of variation of 2.0% for fructose and glucose and of 2.2% for sucrose.

Liquid Chromatographic Determination of Amiodarone Hydrochloride and Related Compounds in Raw Materials and Tablets

1994 ◽  
Vol 77 (6) ◽  
pp. 1447-1453 ◽  
Author(s):  
Pauline M Lacrok ◽  
Norman M Curran ◽  
Wing-Wah Sy ◽  
Dennis K J Goreck ◽  
Pierre Thibault ◽  
...  
Keyword(s):  
Raw Materials ◽  
Chromatographic Determination ◽  
Raw Material ◽  
Liquid Chromatographic Method ◽  
Limit Of Quantitation ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic ◽  
Amiodarone Hydrochloride ◽  
Related Compounds

Abstract A liquid chromatographic method for the determination of amiodarone hydrochloride and 10 related compounds in drug raw material and for assay of drug in tablets was developed. The method specifies a 3 jxm Hypersil nitrile column (150 × 4.6 mm), a mobile phase of 1 + 1 acetonitrile–ammonium acetate buffer (0.1 M adjusted to pH 6.0 with 0.1 M acetic acid), a flow rate of 1 mL/min, and detection at 240 nm. The lower limit of quantitation of the related compounds is 0.02% or less. Drug contents in 2 raw material samples were 100.1 and 99.9% and ranged from 98.2 to 99.4% in 3 tablet formulations. Impurity levels in 2 samples of raw material from different manufacturers were ca 0.4%. The presence of 3 of the known related compounds in these samples was confirmed by liquid chromatographymass spectrometry. The method applied to raw materials was evaluated by a second laboratory and found to be satisfactory.

Sign in / Sign up

Export Citation Format

Share Document