Detection of Paralytic Shellfish Toxins in Southern Rock Lobster Jasus edwardsii Using the Qualitative Neogen™ Lateral Flow Immunoassay: Single-Laboratory Validation

2020 ◽  
Vol 103 (3) ◽  
pp. 784-791
Author(s):  
Juan José Dorantes-Aranda ◽  
Aiko Hayashi ◽  
Alison R Turnbull ◽  
Jessica Y C Jolley ◽  
D Tim Harwood ◽  
...  
Keyword(s):  
Monitoring Program ◽  
Rapid Test ◽  
Probability Of Detection ◽  
Rapid Screening ◽  
Rock Lobster ◽  
Shellfish Toxins ◽  
Paralytic Shellfish Toxins ◽  
Paralytic Shellfish ◽  
Jasus Edwardsii ◽  
Single Laboratory

Abstract Background Paralytic shellfish toxins (PST) are a significant problem for the Tasmanian shellfish and Southern Rock Lobster (Jasus edwardsii) industries, and the introduction of a rapid screening test in the monitoring program could save time and money. Objective The aim was to perform a single-laboratory validation of the Neogen rapid test for PST in the hepatopancreas of Southern Rock Lobster. Methods The AOAC INTERNATIONAL guidelines for the validation of qualitative binary chemistry methods were followed. Three different PST profiles (mixtures) were used, of which two were commonly found in naturally contaminated lobster hepatopancreas (high in gonyautoxin 2&3 and saxitoxin), and the third toxin profile was observed in a few select animals (high in gonyautoxin 1&4). Results The Neogen test consistently returned negative results for non-target toxins (selectivity). The probability of detection (POD) of PST in the lobster hepatopancreas using the Neogen test increased with increasing PST concentrations. POD values of 1.0 were obtained at ≥0.57 mg STX-diHCl eq/kg in mixtures 1 and 2, and 0.95 and 1.0 for mixture 3 at 0.79 and 1.21 mg STX-diHCl eq/kg, respectively, with a fitted POD of 0.98 for 0.80 mg STX-diHCl eq/kg. The performance of the Neogen test when using four different production lots (ruggedness) showed no significant differences. Conclusions The results of the validation study were satisfactory and the Neogen test is being trialed within the Tasmanian PST monitoring program of Southern Rock Lobster. Highlights The Neogen rapid kit was successfully validated for the detection of PST in Southern Rock Lobster hepatopancreas.

Accumulation of paralytic shellfish toxins by Southern Rock lobster Jasus edwardsii causes minimal impact on lobster health

2021 ◽  
Vol 230 ◽  
pp. 105704
Author(s):  
Alison Turnbull ◽  
Navreet Malhi ◽  
Andreas Seger ◽  
Jessica Jolley ◽  
Gustaaf Hallegraeff ◽  
...  
Keyword(s):  
Rock Lobster ◽  
Minimal Impact ◽  
Shellfish Toxins ◽  
Paralytic Shellfish Toxins ◽  
Paralytic Shellfish ◽  
Jasus Edwardsii

scholarly journals Experimental uptake and depuration of paralytic shellfish toxins in Southern Rock Lobster, Jasus edwardsii

Toxicon ◽  
2018 ◽  
Vol 143 ◽  
pp. 44-50 ◽  
Author(s):  
Thomas Madigan ◽  
Navreet Malhi ◽  
Jessica Tan ◽  
Catherine McLeod ◽  
Ian Stewart ◽  
...  
Keyword(s):  
Rock Lobster ◽  
Shellfish Toxins ◽  
Paralytic Shellfish Toxins ◽  
Paralytic Shellfish ◽  
Jasus Edwardsii

Single-Laboratory Validation of the Neogen Qualitative Lateral Flow Immunoassay for the Detection of Paralytic Shellfish Toxins in Mussels and Oysters

2018 ◽  
Vol 101 (2) ◽  
pp. 480-489 ◽  
Author(s):  
Alison R Turnbull ◽  
Jessica Y C Tan ◽  
Sarah C Ugalde ◽  
Gustaaf M Hallegraeff ◽  
Katrina Campbell ◽  
...  
Keyword(s):  
Validation Study ◽  
Rapid Test ◽  
Probability Of Detection ◽  
Alexandrium Tamarense ◽  
Shellfish Toxins ◽  
Paralytic Shellfish Toxins ◽  
Paralytic Shellfish ◽  
Test Kit ◽  
The Matrix ◽  
Bivalve Shellfish

Abstract Detection of paralytic shellfish toxins (PSTs) in bivalve shellfish by analytical methods is complicated and costly, requiring specific expertise and equipment. Following extensive blooms of Alexandrium tamarense Group 1 in Tasmania, Australia, an investigation was made into commercially available screening test kits suitable for use with the toxin profiles found in affected bivalves. The qualitative Neogen rapid test kit, with a modified protocol to convert gonyautoxins GTX1&4 and GTX2&3 into neosaxitoxin and saxitoxin (STX), respectively, with higher cross-reactivities, was the best fit-for-purpose. This validation study of the test kit and the modified protocol was undertaken following AOAC INTERNATIONAL guidelines for the validation of qualitative binary chemistry methods. The validation used four different PST profiles representing natural profiles found in Australia and in Europe: two in a mussel matrix and two in an oyster matrix. The test kit was shown to have appropriate selectivity of the toxin analogs commonly found in bivalve shellfish. The matrix and probability of detection (POD) study showed that the rapid test kit used with the modified protocol was able to consistently detect PST at the bivalve regulatory level of 0.8 mg STX⋅2HCl eq/kg, with a POD estimated via the binomial logistic regression of 1.0 at 0.8 mg STX⋅2HCl eq/kg in all tested profiles in both matrixes. The POD at 0.4 mg STX⋅2HCl eq/kg was 0.75 and 0.46 for the two toxin profiles in an oyster matrix and 0.96 and 1.0 for the two toxin profiles in a mussel matrix. No significant differences in the PODs of the PSTs at the regulatory level were found between production lots of the test kits. The results suggest the method is suitable to undergo a collaborative validation study.

scholarly journals Detection of Paralytic Shellfish Toxins in Mussels and Oysters Using the Qualitative Neogen Lateral-Flow Immunoassay: An Interlaboratory Study

2018 ◽  
Vol 101 (2) ◽  
pp. 468-479 ◽  
Author(s):  
Juan José Dorantes-Aranda ◽  
Jessica Y C Tan ◽  
Gustaaf M Hallegraeff ◽  
Katrina Campbell ◽  
Sarah C Ugalde ◽  
...  
Keyword(s):  
Prediction Interval ◽  
Probability Of Detection ◽  
Interlaboratory Study ◽  
Screening Tests ◽  
Standard Protocol ◽  
Bivalve Molluscs ◽  
Shellfish Toxins ◽  
Paralytic Shellfish Toxins ◽  
Paralytic Shellfish ◽  
The Mean

Abstract Paralytic shellfish toxins (PSTs) in bivalve molluscs represent a public health risk and are controlled via compliance with a regulatory limit of 0.8 mg saxitoxin (STX)⋅2HCl equivalents per kilogram of shellfish meat (eq/kg). Shellfish industries would benefit from the use of rapid immunological screening tests for PSTs to be used for regulation, but to date none have been fully validated. An interlaboratory study involving 16 laboratories was performed to determine the suitability of the Neogen test to detect PSTs in mussels and oysters. Participants performed the standard protocol recommended by the manufacturer and a modified protocol with a conversion step to improve detection of gonyautoxin 1&4. The statistical analysis showed that the protocols had good homogeneity across all laboratories, with satisfactory repeatability, laboratory, and reproducibility variation near the regulatory level. The mean probability of detection (POD) at 0.8 mg STX⋅2HCl eq/kg using the standard protocol in mussels and oysters was 0.966 and 0.997, respectively, and 0.968 and 0.966 using the modified protocol. The estimated LOD in mussels was 0.316 mg STX⋅2HCl eq/kg with the standard and 0.682 mg STX⋅2HCl eq/kg with the modified protocol, and 0.710 and 0.734 mg STX⋅2HCl eq/kg for oysters, respectively. The Neogen test may be acceptable for regulatory purposes for oysters in accordance with European Commission directives in which the standard protocol provides, at the regulatory level, a probability of a negative response of 0.033 on 95% of occasions. Its use for mussels is less consistent at the regulatory level due to the wide prediction interval around the POD.

scholarly journals Field Validation of the Southern Rock Lobster Paralytic Shellfish Toxin Monitoring Program in Tasmania, Australia

Marine Drugs ◽  
2021 ◽  
Vol 19 (9) ◽  
pp. 510
Author(s):  
Alison Turnbull ◽  
Juan José Dorantes-Aranda ◽  
Tom Madigan ◽  
Jessica Jolley ◽  
Hilary Revill ◽  
...  
Keyword(s):  
East Coast ◽  
Market Access ◽  
Monitoring Program ◽  
Maximum Level ◽  
Cost Effective ◽  
Management Program ◽  
Rock Lobster ◽  
Paralytic Shellfish Toxins ◽  
Paralytic Shellfish ◽  
Lobster Fishery

Paralytic shellfish toxins (PST) are found in the hepatopancreas of Southern Rock Lobster Jasus edwardsii from the east coast of Tasmania in association with blooms of the toxic dinoflagellate Alexandrium catenella. Tasmania’s rock lobster fishery is one of the state’s most important wild capture fisheries, supporting a significant commercial industry (AUD 97M) and recreational fishing sector. A comprehensive 8 years of field data collected across multiple sites has allowed continued improvements to the risk management program protecting public health and market access for the Tasmanian lobster fishery. High variability was seen in toxin levels between individuals, sites, months, and years. The highest risk sites were those on the central east coast, with July to January identified as the most at-risk months. Relatively high uptake rates were observed (exponential rate of 2% per day), similar to filter-feeding mussels, and meant that lobster accumulated toxins quickly. Similarly, lobsters were relatively fast detoxifiers, losing up to 3% PST per day, following bloom demise. Mussel sentinel lines were effective in indicating a risk of elevated PST in lobster hepatopancreas, with annual baseline monitoring costing approximately 0.06% of the industry value. In addition, it was determined that if the mean hepatopancreas PST levels in five individual lobsters from a site were <0.22 mg STX equiv. kg−1, there is a 97.5% probability that any lobster from that site would be below the bivalve maximum level of 0.8 mg STX equiv. kg−1. The combination of using a sentinel species to identify risk areas and sampling five individual lobsters at a particular site, provides a cost-effective strategy for managing PST risk in the Tasmanian commercial lobster fishery.

Multiplexed ELISA screening assay for nine paralytic shellfish toxins in human plasma

The Analyst ◽  
2019 ◽  
Vol 144 (15) ◽  
pp. 4702-4707 ◽  
Author(s):  
Padmanabhan Eangoor ◽  
Amruta Sanjay Indapurkar ◽  
Mani Deepika Vakkalanka ◽  
Jennifer Sporty Knaack
Keyword(s):  
Human Plasma ◽  
Paralytic Shellfish Poisoning ◽  
Rapid Screening ◽  
Shellfish Poisoning ◽  
Screening Assay ◽  
Shellfish Toxins ◽  
Paralytic Shellfish Toxins ◽  
Paralytic Shellfish

Paralytic shellfish poisoning is a lethal syndrome that can develop in humans who consume shellfish contaminated with paralytic shellfish toxins. This rapid screening assay can be used to quickly diagnose exposure to paralytic shellfish toxins.

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