scholarly journals An Escherichia coli ribonuclease which removes an extra nucleotide from a biosynthetic intermediate of bacteriophage T4 proline transfer RNA

1978 ◽  
Vol 5 (11) ◽  
pp. 4129-4140 ◽  
Author(s):  
Francis J. Schmidt ◽  
William H. McClain
1973 ◽  
Vol 19 (4) ◽  
pp. 421-426 ◽  
Author(s):  
E. R. Roback ◽  
J. D. Friesen ◽  
N. P. Fill

A method of selecting temperature-sensitive mutants of Escherichia coli which makes use of a double temperature shift combined with suicide as a result of incorporated 3H-uridine is described. One mutant selected in this way shows restricted growth at 42 °C resulting from early inhibition of protein and ribonucleic acid (RNA) synthesis. In vitro assays of aminoacyl-transfer RNA synthetase activity indicated a complete absence of active glycyl-transfer RNA synthetase in the mutant. This enzyme activity in the mutant was distinguishable from the wild type by its rapid inactivation at 28 °C in cell-free extracts. Infection of the temperature-sensitive mutant with bacteriophage T4 did not alter the heat-sensitivity of the mutant enzyme. The mutation is 72% cotransducible with the xyl locus.


ACS Sensors ◽  
2021 ◽  
Author(s):  
Pallavi Halkare ◽  
Nirmal Punjabi ◽  
Jigme Wangchuk ◽  
Santhosh Madugula ◽  
Kiran Kondabagil ◽  
...  

1974 ◽  
Vol 90 (4) ◽  
pp. 665-676 ◽  
Author(s):  
M.Margaret Comer ◽  
Christine Guthrie ◽  
William H. McClain

2009 ◽  
Vol 26 (3) ◽  
pp. 865-871
Author(s):  
Dominic Sauvageau ◽  
B��atrice Allain ◽  
David G. Cooper

Biochemistry ◽  
1973 ◽  
Vol 12 (3) ◽  
pp. 372-380 ◽  
Author(s):  
John S. Erickson ◽  
Christopher K. Mathews

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