Studies on Algal Cytochromes. V. Purification and Characterization of Cytochrome c-552 from a Red Alga, Polysiphonia urceolata1

1984 ◽  
Vol 96 (6) ◽  
pp. 1681-1687 ◽  
Author(s):  
Yasutomo SUGIMURA ◽  
Ken HOSOYA ◽  
Fuminori YOSHIZAKI ◽  
Masami SHIMOKORIYAMA
1997 ◽  
Vol 52 (11-12) ◽  
pp. 740-746 ◽  
Author(s):  
Röbbe Wünschiers ◽  
Thomas Zinn ◽  
Dietmar Linder ◽  
Rüdiger Schulz

Abstract Purification of a soluble cytochrome c6 from the unicellular green alga Scenedesmus obliquus by a simple and rapid method is described. The purification procedure includes ammonium sulfate precipitation and non-denaturating PAGE. The N-terminal sequence of the first 20 amino acids was determined and shows 85% similarity and 75% identity to the sequence of cytochrome c6 from the green alga Monoraphidium braunii. The ferrocyto-chrome shows typical UV/VIS absorption peaks at 552.9, 521.9 and 415.7 nm. The apparent molecular mass was estimated to be 12 kD a by SDS-PAGE. EPR-spectroscopy at 20K shows resonances indicative for two distinct low-spin heme forms.


2001 ◽  
Vol 3 (5) ◽  
pp. 493-500 ◽  
Author(s):  
Koji Iwamoto ◽  
Hideaki Kawanobe ◽  
Yoshihiro Shiraiwa ◽  
Tomoyoshi Ikawa

1996 ◽  
Vol 98 (4) ◽  
pp. 753-758 ◽  
Author(s):  
Pavel V. Prosselkov ◽  
Wolfgang Gross ◽  
Abir U. Igamberdiev ◽  
Claus Schnarrenberger

1981 ◽  
Vol 10 (3) ◽  
pp. 267-270 ◽  
Author(s):  
Kazunobu Matsushita ◽  
Kenji Tayama ◽  
Emiko Shinagawa ◽  
Osao Adachi ◽  
Minoru Ameyama

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