First Report of Citrus Tristeza Virus in Louisiana.

Plant Disease ◽  
1996 ◽  
Vol 80 ◽  
pp. 103 ◽  
Author(s):  
R. A. Valverde
Keyword(s):  
Citrus Tristeza Virus ◽  
First Report ◽  
Tristeza Virus ◽  
Citrus Tristeza

First report of citrus tristeza virus in commercial citrus orchards in Tunisia

2021 ◽  
Author(s):  
Asma Najar ◽  
Imen Hamdi ◽  
Souad Mahmoud ◽  
Lassaad Medhioub ◽  
Imed Jaouadi ◽  
...  
Keyword(s):  
Citrus Tristeza Virus ◽  
First Report ◽  
Citrus Orchards ◽  
Tristeza Virus ◽  
Citrus Tristeza

scholarly journals Correction to: First report of citrus tristeza virus trifoliate resistance-breaking (RB) genotype in Citrus grandis in China

2018 ◽  
Vol 101 (2) ◽  
pp. 453-453
Author(s):  
Jun Wang ◽  
Tianyu Zhou ◽  
Mengji Cao ◽  
Yan Zhou ◽  
Zhongan Li
Keyword(s):  
Citrus Tristeza Virus ◽  
First Report ◽  
Resistance Breaking ◽  
Tristeza Virus ◽  
Citrus Tristeza

scholarly journals First Report from Syria of Citrus tristeza virus in Citrus spp.

Plant Disease ◽  
2008 ◽  
Vol 92 (10) ◽  
pp. 1468-1468
Author(s):  
R. Abou Kubaa ◽  
K. Djelouah ◽  
A. M. D'Onghia ◽  
R. Addante ◽  
M. Jamal
Keyword(s):  
Citrus Tristeza Virus ◽  
Sweet Orange ◽  
Citrus Limon ◽  
Potential Threat ◽  
First Report ◽  
Mexican Lime ◽  
Vein Clearing ◽  
Citrus Varieties ◽  
Tristeza Virus ◽  
Citrus Tristeza

During the spring of 2006, the main Syrian citrus-growing areas of Lattakia (Jableh, Aledyye, Eseelya, Siano, and Hresoon provinces) and Tartous (Almintar, Aljammase, Karto, Majdaloonelbahr, Yahmour, Amreet, Althawra, and Safita provinces) were surveyed to assess the presence of Citrus tristeza virus (CTV). Eight nurseries (approximately 130 plants per nursery), two budwood source fields (approximately 230 trees per field), and 19 groves (approximately 60 trees per grove) containing the main citrus varieties were visually inspected and sampled for serological assays. The hierarchical sampling method was carried out in each selected grove (2). Infected samples were collected from two nurseries, two budwood source fields, and six groves. Stems and leaf petioles from nursery trees and flower explants from the groves were collected and analyzed for CTV by direct tissue blot immunoassay (DTBIA) with the commercial kit from Plantprint (Valencia, Spain). Of 2,653 samples tested, 89 (4%) CTV-infected plants were detected. Five citrus varieties were found to be infected and Meyer lemon (Citrus limon ‘Meyer’) had the highest incidence at 16%. Numerous sweet orange varieties (Citrus sinensis L.) were found to be highly infected in the field, but only the Washington navel sweet orange was found to be infected in the nurseries. No clear CTV symptoms were observed during the survey. Samples that were positive for CTV by DTBIA were also positive by biological indexing on Mexican lime (C. aurantifolia) and immunocapture-reverse transcription-PCR as described by Nolasco et al. (3). Coat protein gene sequences obtained from five selected clones of a Syrian CTV isolate (GenBank Accession No. EU626555) showed more than 99 and 98% nucleotide sequence identity to a Jordanian CTV isolate (GenBank Accession No. AY550252) and the VT isolate (GenBank Accession No. U56902), respectively. Almost all infected samples induced moderate vein clearing symptoms when grafted to Mexican lime. Symptoms of vein clearing, leaf cupping, stunting, and stem pitting on Mexican lime were induced by graft transmission of CTV from one Valencia sample from the Tartous area. The viral inoculum is widely and randomly distributed in commercial groves, especially in the southern Tartous area and in some nurseries. To our knowledge, this is the first report of CTV in Syria. However, CTV was reported from the neighboring citrus-growing countries of Lebanon, Turkey, and Jordan (1), and the severe seedling yellows strain is present in this area, which poses a potential threat to Syrian citriculture. References: (1) G. H. Anfoka et al. Phytopathol. Mediterr. 44:17, 2005. (2) G. Hughes and T. R. Gottwald, Phytopathology 88:715, 1998. (3) G. Nolasco et al. Eur. J. Plant Pathol. 108:293, 2002.

scholarly journals First Report of Citrus tristeza virus in Greece

Plant Disease ◽  
2002 ◽  
Vol 86 (3) ◽  
pp. 329-329 ◽  
Author(s):  
D. Dimou ◽  
J. Drossopoulou ◽  
E. Moschos ◽  
C. Varveri ◽  
F. Bem
Keyword(s):  
Citrus Tristeza Virus ◽  
Sweet Orange ◽  
Enzyme Linked Immunosorbent Assay ◽  
First Report ◽  
Vein Clearing ◽  
Carrizo Citrange ◽  
Sweet Lime ◽  
Tristeza Virus ◽  
Propagation Material ◽  
Citrus Tristeza

Large-scale surveys of Citrus spp. for the presence of Citrus tristeza virus (CTV) by the Ministry of Agriculture in Greece began in 1995. Over 26,000 trees have been tested by enzyme-linked immunosorbent assay and immunoprinting (2). In summer 2000, the first CTV-infected sweet orange cv. Lane Late tree grafted on CTV-tolerant Carrizo citrange was found in Argolis County, Peloponnese. This tree belonged to a batch of CAC propagation material (20 trees) illegally introduced from Spain in 1994, which was subsequently traced and found to be infected (45%). A follow-up search of trees grafted with the above material was undertaken in the two concerned regions (Argolis and Chania-Crete), and more than 3,500 trees have been removed. Extensive surveys continue to identify and destroy new infections. Few cases (15 of 16,800) of natural transmission to cultivars other than cv. Lane Late have been found. All of these have been close to the initially infected trees in the Argolis area. Surveys in spring 2001 were extended to certified propagation material of Clemenpons mandarin on Carrizo citrange imported from Spain, and 7 of 1,038 plants were infected (0.64%). The virus was successfully graft-transmitted to sweet orange cv. Madame Vinous and sweet lime seedlings, where it was identified by immunoprinting and reverse transcription-polymerase chain reaction (1). Mild vein clearing symptoms appeared on both indicators. Vein clearing on sweet lime was also accompanied by leaf cupping. To our knowledge, this is the first report of CTV in Greece. References: (1) A. Sambade et al. J. Virol. Methods 87:25, 2000. (2) C. Vela et al. J. Gen. Virol. 67:91, 1986.

scholarly journals First report of the Citrus tristeza virus resistance-breaking strain in Morocco

2018 ◽  
Vol 100 (2) ◽  
pp. 351-351 ◽  
Author(s):  
Mohamed Afechtal ◽  
Anna Maria D’Onghia ◽  
Giuseppe Eros Massimino Cocuzza ◽  
Khaled Djelouah
Keyword(s):  
Virus Resistance ◽  
Citrus Tristeza Virus ◽  
Breaking Strain ◽  
First Report ◽  
Resistance Breaking ◽  
Citrus Tristeza Virus Resistance ◽  
Tristeza Virus ◽  
Citrus Tristeza

scholarly journals First Report of the Citrus tristeza virus Trifoliate Resistance-Breaking (RB) Genotype in ‘Newhall’ Sweet Orange in South America

Plant Disease ◽  
2017 ◽  
Vol 101 (6) ◽  
pp. 1063 ◽  
Author(s):  
L. Hernández-Rodríguez ◽  
A. Bertalmío ◽  
A. Arruabarrena ◽  
L. Rubio ◽  
F. Rivas ◽  
...  
Keyword(s):  
South America ◽  
Citrus Tristeza Virus ◽  
Sweet Orange ◽  
First Report ◽  
Resistance Breaking ◽  
Tristeza Virus ◽  
Citrus Tristeza

scholarly journals First Report of Citrus tristeza virus (CTV) in Greece

2002 ◽  
Vol 15 (15) ◽  
Author(s):  
D. Dimou ◽  
J. Drosopoulou ◽  
E. Moschos ◽  
C. Spanou ◽  
P. Dermatas
Keyword(s):  
Citrus Tristeza Virus ◽  
First Report ◽  
Tristeza Virus ◽  
Citrus Tristeza

scholarly journals First Report of Citrus tristeza virus Infecting Citrus Trees in Georgia, USA

Plant Disease ◽  
2021 ◽  
Author(s):  
Emran Ali ◽  
Allina Bennett ◽  
Tammy Stackhouse ◽  
Sumyya Waliullah ◽  
Jonathan E Oliver
Keyword(s):  
Plant Material ◽  
Citrus Tristeza Virus ◽  
Infected Plant ◽  
Poncirus Trifoliata ◽  
First Report ◽  
Leaf Material ◽  
Tristeza Virus ◽  
Infected Plant Material ◽  
Citrus Tristeza ◽  
Citrus Trees

Citrus tristeza virus (CTV) [genus Closterovirus; family Closteroviridae] is one of the most important, economically devastating viruses of citrus worldwide. On citrus trees grafted onto sour orange rootstock, typical CTV symptoms include dieback and defoliation, stunting, curling and chlorotic leaves, stem-pitting, and pinholes below the bud union on the inner face of the bark (Moreno et al. 2008). This single-stranded, positive-sense RNA virus is most efficiently transmitted by the brown citrus aphid (Toxoptera citricida), but it can also be transmitted by other aphid species and through grafting of infected plant material onto healthy plants (Moreno et al 2008; Herron et al. 2006). In Fall 2020, leaf material for virus testing was collected from 13 navel orange trees (Citrus × sinensis) grafted onto Poncirus trifoliata rootstocks (including ‘Flying Dragon’) located in a citrus research orchard in Tifton, GA. Trees ranged in age from 2 to 10 years, with the younger trees having been grafted from cuttings taken from the older trees. The oldest of these trees was derived from cuttings taken in 2009 from an orange tree growing locally in a residential yard in Tifton; this parent tree was more than 15 years old when these cuttings were obtained and was no longer available for sampling as of 2020. Symptoms or other visible signs of disease had not been noted on any of the tested trees, and trees were chosen for testing prior to the further dissemination of this plant material. The presence of CTV was verified via molecular and serological testing. CTV infection was initially confirmed in 8 of 13 tested samples using the ImmunoStrip® for CTV assay (Agdia® Inc., Elkhart, IN, cat no: ISK 78900/0025) according to the manufacturer’s instructions. RNA was extracted from leaf material collected from the 13 sampled trees using the RNeasy Plant Mini Kit (Qiagen, Valencia, CA). Following cDNA synthesis, samples were tested for the presence of CTV by reverse-transcription PCR using primer pair AR18F (5’-ATGTCAGGCAGCTTGGGAAATT-3’) and AR18R (5’-TTCGTGTCTAAGTCRCGCTAAACA-3’) which produces a 511 bp amplicon (Roy et al., 2005). PCR reactions confirmed the presence of CTV, with the same eight samples that had previously tested positive via Immunostrip® producing PCR fragments of the expected size. Amplified products from two of these samples were then sequenced using Sanger sequencing (Retrogen Inc, San Diego, CA, USA) and subjected to BLAST analysis (https://blast.ncbi.nlm.nih.gov/Blast.cgi) for further identification. Sequence analysis revealed that the obtained partial sequences (MW540805) from the p18 gene of both isolates were 100% identical to one another and shared 100% identity to corresponding sequences from CTV strain N4 (MK779711.1). To the best of our knowledge, this is the first report of CTV infecting citrus plants in Georgia. CTV could pose an imminent threat to the emerging citrus industry in Georgia if it were to become established in commercial citrus plantings either via the dissemination of infected plant material or via vector transfer of the virus under field conditions. While the brown citrus aphid is not known to be widespread in Georgia at this time, other CTV vectors are prevalent including the cotton aphid (Aphis gossypii) and the black citrus aphid (T. aurantia). Georgia citrus growers and plant propagators should be aware of this virus and take appropriate control measures to prevent the spread of this viral diseas.

First Report of Citrus tristeza virus (CTV) Disease in Commercial Citrus Orchards in Sudan

2017 ◽  
Vol 3 (1) ◽  
pp. 1-11
Author(s):  
Mohamed Abubaker ◽  
Siddig Elhassan ◽  
Awadalla Irabi
Keyword(s):  
Citrus Tristeza Virus ◽  
First Report ◽  
Citrus Orchards ◽  
Tristeza Virus ◽  
Citrus Tristeza

First report of citrus tristeza virus in Lao PDR

2021 ◽  
Author(s):  
N. J. Donovan ◽  
A. Englezou ◽  
G. A. Chambers ◽  
S. Phanthavong ◽  
A. Daly ◽  
...  
Keyword(s):  
Citrus Tristeza Virus ◽  
Lao Pdr ◽  
First Report ◽  
Tristeza Virus ◽  
Citrus Tristeza
Sign in / Sign up

Export Citation Format

Share Document