Citrus Diseases Exotic to Florida: Citrus Tristeza Virus–Stem Pitting (CTV-SP)

This document is one in a series designed to provide important information on the causal agent, symptoms, and transmission of exotic citrus diseases. This information can be used as an educational tool to raise awareness about these diseases and for scouting and identification efforts. Disseminating information about the diseases to the citrus industry may prevent their introduction and spread in Florida. This document will focus on the exotic viral disease caused by isolates of citrus tristeza virus–stem pitting (CTV-SP). Original version: Chung, Kuang-Ren, and Ronald Brlansky. 2006. “Citrus Diseases Exotic to Florida: Citrus Tristeza Virus– Stem Pitting (CTV-SP)”. EDIS 2006 (7). https://doi.org/10.32473/edis-pp149-2006.

La importancia de la aplicación de micorrizas para disminuir la abscisión de naranja (Citrus sinensis L. Osbeck) en Veracruz, México / The importance of the application of mycorrhizae to reduce abscission of orange in Veracruz, Mexico

México es el cuarto productor de cítricos a nivel mundial (FAOSTAT, 2019) y el estado de Veracruz aporta el 53 % de la producción nacional (SIAP, 2019). El problema de la abscisión de frutos de naranja cobró importancia en 2017, pero ya existía este problema desde hace 15 años, lo cual ocasiona pérdidas en la producción del 20% en la zona Norte de Veracruz (Gómez et al., 2020). El ataque de patógenos: Colletotrichum spp, Botryodiplodia spp, Fusarium spp, Citrus tristeza virus (VTC), Candidatus Liberibacter asiaticus (CLas), Citrus exocortis viroid (CEVd) y Hop stunt viroid (HSVd); pueden influir, entre otros factores, en la caída de naranja. El experimento se llevó a cabo en la huerta `Grupo Los Gómez’ en el ejido de San Pablo, Papantla, Veracruz, México; en naranja ‘Valencia’ portainjerto ‘Agrio’ y en suelo tipo aluvión. El objetivo central de la investigación fue evaluar 15 insumos agroecológicos para disminuir la abscisión de naranja, 7 de estos insumos con micorrizas y el resto sin su aplicación. Los tratamientos con mejores resultados fueron: Agua de vidrio (1.25 %) + Micorriza; Optifer amino (0.5 %) + Micorriza; y EM (5 %) + Micorriza con menor porcentaje de caída de naranja con 4.97%, 5.79%, 6.10% respectivamente.

The Intriguing Conundrum of a Nonconserved Multifunctional Protein of Citrus Tristeza Virus That Interacts with a Viral Long Non-Coding RNA

Citrus tristeza virus (CTV), the largest non-segmented plant RNA virus, has several peculiar features, among which is the production of a 5′-terminal long non-coding RNA (lncRNA) termed low-molecular-weight tristeza 1 (LMT1). In this study, we found that p33, a unique viral protein that performs multiple functions in the virus infection cycle, specifically binds LMT1, both in vivo and in vitro. These results were obtained through the expression of p33 under the context of the wild type virus infection or along with a mutant CTV variant that does not produce LMT1 as well as via ectopic co-expression of p33 with LMT1 in Nicotiana benthamiana leaves followed by RNA immunoprecipitation and rapid amplification of cDNA ends assays. Further experiments in which a recombinant p33 protein and an in vitro transcribed full-length LMT1 RNA or its truncated fragments were subjected to an electrophoretic mobility shift assay demonstrated that p33 binds to at least two distinct regions within LMT1. To the best of our knowledge, this is the first report of a plant virus protein binding to a lncRNA produced by the same virus. The biological significance of the interaction between these two viral factors is discussed.

Incidence of Citrus Tristeza Virus and its Vector Toxoptera citricida in Different Parts of Assam and Nagaland, India

A field survey was conducted in 2018 to study the incidence of citrus tristeza virus (CTV) and its aphid vectors, in different citrus growing regions of the states of Assam and Nagaland, India. Leaf samples of Assam lemon (Citrus limon) and Khasi mandarin (Citrus reticulata) were collected from four districts of Assam (Jorhat, Tinsukia, Sivasagar and Golaghat) and two districts of Nagaland (Mokokchung and Wokha). Citrus leaf samples were collected from a total of 190 citrus plants and were used for detection of CTV infection through Double Antibody Sandwich-Enzyme linked Immuno-Sorbent Assay (DAS-ELISA). According to the results, 75 per cent CTV disease incidence was detected in surveyed areas of Assam and 24.55 per cent CTV disease incidence was detected in surveyed areas of Nagaland. District wise, the highest CTV disease incidence (96.67 %) was detected in Tinsukia district of Assam and the lowest (21.43 %) was detected in Mokokchung district of Nagaland. Aphid samples were also collected during the survey and the presence of the vector Toxoptera citricida, in all the locations was determined.