First Report of Fusarium oxysporum Causing Root Rot in Ophiopogon bodinieri in China

A new wilt and root rot disease was observed in 6 and 11 commercial fields of lettuce (Lactuca sativa) in western Arizona during the fall of 2001 and 2002, respectively. Distance between infested sites ranged from approximately 0.5 to 39 km. Five head lettuce cultivars as well as a red leaf lettuce cultivar were affected. Disease symptoms included yellowing and wilting of leaves, as well as stunting and plant death. The cortex of the crown and upper root of infected plants usually was decayed and reddish brown. Disease symptoms first appeared at the time of plant thinning and continued to develop up to plant maturity. Fusarium oxysporum was consistently isolated from symptomatic plant roots. Seeds of cv. Lighthouse were planted in nonsterile vermiculite within 3.0-cm-square × 7.0-cm-deep cells in a transplant tray and thinned to a single plant per cell. When the first true leaves were emerging, 10 individual seedlings were inoculated with a single-spore isolate of F. oxysporum recovered from diseased lettuce root cortex tissue. Inoculum was prepared by growing the fungus on potato dextrose agar in 100-mm-diameter × 15-mm-deep plastic petri dishes at 28°C with a 12-h photoperiod under fluorescent light. Once the fungus completely covered the agar surface, 50 ml of sterile distilled water was added to the dish, and the mycelia and conidia on the surface were scraped off the agar and suspended in the water. This fungal suspension was decanted, and a 2-ml aliquot containing 1.8 × 105 CFU was pipetted into the vermiculite near the stem of each lettuce seedling. Ten plants grown in noninfested vermiculite served as uninoculated controls. After inoculation, plants were maintained in a growth chamber at 28°C with a 12-h photoperiod under fluorescent light for 3 weeks. Symptoms of yellowing, wilt, vascular decay, and often plant death developed during the incubation period on all inoculated plants but not on control plants. Fusarium oxysporum was consistently reisolated from inoculated plants but not from uninoculated plants. The experiment was repeated and yielded the same results. A wilt and root rot disease of lettuce attributed to F. oxysporum f. sp. lactucae was first reported in Japan in 1967 (3) and subsequently in the United States (San Joaquin Valley of California) in 1993 (2), and Italy in 2002 (1). The researchers of the U.S. report did not cite the earlier work from Japan and described the pathogen as F. oxysporum f. sp. lactucum. The Arizona isolate used to demonstrate pathogenicity was of the same vegetative compatibility group as an isolate of the pathogen from lettuce in California reported in 1993. Several companies grow and harvest lettuce in Arizona and California. At the end of production and harvest in the fall, tractors, implements, and harvesting equipment are transported from the San Joaquin Valley in California to western Arizona. The similarity between the isolate of F. oxysporum f. sp. lactucae from western Arizona and the San Joaquin Valley of California suggest a possible introduction of the pathogen into Arizona from California, perhaps on soil adhering to farm equipment. To our knowledge, this is the first report of F. oxysporum f. sp. lactucae infecting lettuce in Arizona. References: (1) A. Garibaldi et al. Plant Dis. 86:1052, 2002. (2) J. C. Hubbard and J. S. Gerik. Plant Dis. 77:750, 1993. (3) T. Matuo and S. Motohashi. Trans. Mycol. Soc. Jpn. 8:13, 1967.

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First report of root rot caused by Fusarium oxysporum on Macleaya cordata in China

Journal of Plant Pathology ◽

10.1007/s42161-019-00382-8 ◽

2019 ◽

Vol 102 (1) ◽

pp. 191-192

Author(s):

Li Zhou ◽

Peng Huang ◽

Linlan Yu ◽

Jianguo Zeng

Keyword(s):

Fusarium Oxysporum ◽

Root Rot ◽

First Report ◽

Macleaya Cordata

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First Report of Fusarium Crown and Root Rot on Torreya grandis Caused by Fusarium oxysporum Species Complex in China

Plant Disease ◽

10.1094/pdis-12-15-1514-pdn ◽

2016 ◽

Vol 100 (7) ◽

pp. 1500-1500 ◽

Cited By ~ 1

Author(s):

C. Q. Zhang ◽

S. Y. Zhang ◽

X. L. Chen ◽

Q. Q. Qi ◽

H. Z. Lou

Keyword(s):

Fusarium Oxysporum ◽

Root Rot ◽

Species Complex ◽

First Report ◽

Torreya Grandis ◽

Crown And Root Rot ◽

Fusarium Oxysporum Species Complex

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First Report of Fusarium Root Rot Caused by Fusarium oxysporum Infecting Pigmented Grapefruit Trees in Turkey

Plant Disease ◽

10.1094/pdis-07-14-0746-pdn ◽

2015 ◽

Vol 99 (4) ◽

pp. 553-553

Author(s):

F. Baysal-Gurel ◽

A. Cinar

Keyword(s):

Fusarium Oxysporum ◽

Root Rot ◽

Fusarium Root Rot ◽

First Report

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First Report of Stem Wilt and Root Rot of Schlumbergera truncata Caused by Fusarium oxysporum f. sp. Opuntiarum in Southern Italy

Plant Disease ◽

10.1094/pdis-11-12-1092-pdn ◽

2013 ◽

Vol 97 (6) ◽

pp. 846-846 ◽

Cited By ~ 6

Author(s):

F. Lops ◽

F. Cibelli ◽

M. L. Raimondo ◽

A. Carlucci

Keyword(s):

Fusarium Oxysporum ◽

Root Rot ◽

Southern Italy ◽

Nucleotide Sequences ◽

Control Treatment ◽

Conidial Suspension ◽

Internal Transcribed Spacer Region ◽

Fungal Isolation ◽

First Report ◽

Fusarium Sp

Schlumbergera truncata (Haw.) Moran, belonging to the Cactaceae, is a very common ornamental cactus in southern Italy. In November 2011, sudden stem wilt and root rot was observed in about 45% of vegetatively propagated plants cultivated as potted ornamental plants in a commercial greenhouse in Cerignola (Foggia Province, Apulia, Italy). The roots and collars of the plants showed brown rot. Yellow sunken lesions that were similar to cortical cankers were detected at basal level of the stem. Ten plants with these symptoms were analyzed by fungal isolation techniques. Small (0.5 cm) tissue portions from root, collar, and basal stem were plated on potato dextrose agar (PDA) after disinfection with 75% ethanol for 1 to 2 min, 0.2% NaOCl for 1 to 2 min, and a wash with sterile distilled water. A fungal isolate that was morphologically similar to Fusarium sp. was isolated from 85% of these tissue samples. It had nucleotide sequences of the internal transcribed spacer region (ITS1-5.8S-ITS2) of ribosomal DNA (GenBank Accession No. KC196121) 100% identical to those of the comparable sequences of Fusarium oxysporum (HQ651161). The nucleotide sequences of its translation elongation factor 1-α (EF-1α) gene (KC196120) showed 100% identity to sequences of F. oxysporum f. sp. opuntiarum (DQ837689, AF246881) retrieved from GenBank. Pathogenicity tests were performed at 22 ± 3°C on 18 45-day-old plants of S. truncate by adding of a 5-ml aliquot of conidial suspension adjusted to 5 × 106 conidia/ml to soil of each plant. Six non-inoculated plants were used for a control treatment and sprayed with 5 ml of sterilized water. Plants were maintained in greenhouse at 22 ± 3°C. After 10 days, nine of the inoculated plants showed wilting, and after 45 days, all of them were dead, with root and collar rot and lesions on the basal stem. Control plants were symptomless. Koch's postulates were fulfilled as the pathogen was reisolated from all of the symptomatic tissues and identified as Fusarium sp. On the basis of 3-septate macroconidia (mean 31.75 × 3.21 μm; range, 26 to 35 μm long, 3.0 to 4.2 μm wide), aseptate microconidia, single chlamydospores, and monophialide conidiophores on carnation leaf agar, and molecular analyses, the fungus was identified as F. oxysporum f. sp. opuntiarum (Speg) (1,2,3). In Italy, F. oxysporum f. sp. opuntiarum was reported as basal stem rot of Echinocactus grusoni (4). To our knowledge, this is the first report of stem wilt and root rot of S. truncata caused by F. oxysporum f. sp. opuntiarum in Italy. References: (1) W. Gerlach. Phytopathol. Z. 74:197, 1972. (2) W. L. Gordon. Can. J. Bot. 43:1309, 1965. (3) P. E. Nelson et al. Fusarium Species: An Illustrated Manual for Identification. Pennsylvania State University Press, University Park, 1983. (4) G. Polizzi et al. Plant Dis. 88:85, 2004.

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