scholarly journals Natural Occurrence of the Entomopathogenic Fungus Nomuraea rileyi in the Soybean Green Semilooper, Chrysodeixis acuta, in India

2003 ◽  
Vol 4 (1) ◽  
pp. 42 ◽  
Author(s):  
Ved Prakash Gupta

Nomuraea rileyi (Farlow) Samson is an entomopathogenic fungus that infects lepidopteran larvae. The fungus was observed in populations of the soybean green semilooper, Chrysodeixis acuta, in the humid Southeastern Plain Zone of Rajasthan, India, in 2001. Accepted for publication 16 December 2002. Published 13 January 2003.

FLORESTA ◽  
2011 ◽  
Vol 41 (1) ◽  
Author(s):  
Larissa Rolim Borges ◽  
Sônia Maria Noemberg Lazzari ◽  
Ida Chapaval Pimentel ◽  
Lívia Cardoso Bueno de Camargo ◽  
Lílian Mattos Kalinoski

O controle da broca-da-erva-mate, Hedypathes betulinus, limita-se ao emprego de práticas silviculturais e catação manual dos insetos adultos, não sendo permitido o uso de inseticidas químicos na cultura. Esta pesquisa teve por objetivos verificar a presença natural de Beauveria bassiana no solo de plantio em monocultura de erva-mate, determinar a persistência de B. bassiana no solo após duas e três aplicações do fungo e averiguar a influência de fatores abióticos na estabilidade do fungo no solo. O estudo foi conduzido em um erval no município de Campo Alegre, Santa Catarina, Brasil, onde foram coletadas amostras de solo em duas áreas, para avaliação da presença natural de inóculos do fungo B. bassiana, procedendo-se à contagem, isolamento e identificação.Não foi detectada a ocorrência natural do fungo no solo em nenhuma das duas áreas. Após essa confirmação, foram realizadas pulverizações com B. bassiana em ambas as áreas, sendo que em uma ocorreram duas e na outra três aplicações. Após a última pulverização, realizada em fevereiro/2006, verificou-se que os inóculos do fungo persistiram por 90 dias na área com duas aplicações e 120 dias na área com três aplicações. A temperatura afetou a persistência do fungo no solo em ambas as áreas, mas a umidade relativa não mostrou influência sobre os inóculos em campo.Palavras-chave: Broca-da-erva-mate; controle biológico; epizootia; fungo entomopatogênico. AbstractPersistence in soil of inoculum of Beauveria bassiana used to control Hedypathes betulinus in yerba maté plantation, Ilex paraguariensis. Control measures of Hedypathes betulinus are restricted to agricultural practices and hand picking of adult insects. No chemical control is allowed in yerba maté cultivation areas. The objectives of this research were to verify the natural occurrence of the entomopathogenic fungus Beauveria bassiana in the soil of yerba maté in monoculture; to determine the persistence of inoculums in the soil after applications of the fungus; and to evaluate the influence of abiotic factors on fungus stability in the soil. The soil samples were taken from two experimental areas in a yerba maté plantation in the county of Campo Alegre, Santa Catarina, Brazil. The samples were analyzed in order to count, isolate and identify inoculums of B. bassiana and other fungi. After the confirmation that B. bassiana was not present in the soil, a suspension of the fungus was applied, twice in one of the areas and three times in the other. After the last application in February/2006, the inoculum persisted for 90 and 120 days, in the area with two and three applications, respectively. The temperature affected the persistence of B. bassiana in soil; however, the relative humidity did not.Keywords: Biological control; epizooty; entomopathogenic fungus; wood borer. 


1993 ◽  
Vol 122 (3) ◽  
pp. 149-152 ◽  
Author(s):  
G. N. El-Sayed ◽  
C. M. Ignoffo ◽  
T. D. Leathers ◽  
S. C. Gupta

2015 ◽  
Vol 83 ◽  
pp. 19-25 ◽  
Author(s):  
Changwen Shao ◽  
Youping Yin ◽  
Zhaoran Qi ◽  
Ren Li ◽  
Zhangyong Song ◽  
...  

2007 ◽  
Vol 49 ◽  
pp. 67-68
Author(s):  
Naofumi Hozumi ◽  
Yoko Kawamura ◽  
Yoshinari Enami

1978 ◽  
Vol 7 (5) ◽  
pp. 724-727 ◽  
Author(s):  
C. M. Ignoffo ◽  
Clemente Garcia ◽  
D. L. Hostetter ◽  
R. E. Pinnell

1981 ◽  
Vol 27 (3) ◽  
pp. 364-366 ◽  
Author(s):  
D. J. Joslyn ◽  
D. G. Boucias

The zymogram technique has been applied to three pathotypes of the entomopathogenic fungus Nomuraea rileyi. Isozyme profiles of isolates from Heliothis zea, Pseudoplusia includens, and Anticarsia gemmatalis were compared for 17 enzymes of known metabolic function. Electrophoretic data supported the taxonomic differences inferred for the three pathotypes based on host specificity. The isolate from A. gemmatalis was found consistently to be the most distinct.This study demonstrates that isozyme analysis may be used to distinguish closely related fungal isolates of N. rileyi.


2002 ◽  
Vol 45 (3) ◽  
pp. 269-275 ◽  
Author(s):  
Sideney Becker Onofre ◽  
Raul Riveros Gonzalez ◽  
Cláudio Luiz Messias ◽  
João Lúcio Azevedo ◽  
Neiva Monteiro de Barros

The entomopathogenic fungus Nomuraea rileyi (Farlow) Samson produced a peptide active against Anticarsia gemmatalis 3rd instar larvae. To produce this peptide, N. rileyi was cultivated aerobically in Saboraud, maltose, yeast-extract broth at 26 ± 1ºC for 12 days, after which the medium was filtered and separated in a liquid/liquid extractor, concentrated and the peptide purified chromatographically. The crystals obtained were kept refrigerated until needed for LC50 analysis. The LC50 of this peptide against A. gemmatalis 3rd instar larvae was determined in triplicate experiments using solutions containing 1.0, 0.2, 0.1, 0.01, 0.001 and 0.0001 mg/ml of N. rileyi peptide. The results of these experiments were used to calculate a linear equation in which Y = 6,81176 + 1,01382 * LOGx , giving a LC50 value of 0.0163 mg/ml.


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