Induction of Hairy Roots on Cultivated Soybean Genotypes and Their Use to Propagate the Soybean Cyst Nematode

1990 ◽  
Vol 80 (5) ◽  
pp. 503 ◽  
Author(s):  
M. A. Savka
Keyword(s):  
Hairy Roots ◽  
Soybean Cyst Nematode ◽  
Cyst Nematode ◽  
Soybean Genotypes ◽  
Cultivated Soybean

Identification of novel haplotype of a cyst nematode resistance gene, GmSNAP18 in soybean [Glycine max (L.) Merr.]

2020 ◽  
Vol 80 (03) ◽  
Author(s):  
Ik-Young Choi ◽  
Prakash Basnet ◽  
Hana Yoo ◽  
Neha Samir Roy ◽  
Rahul Vasudeo Ramekar ◽  
...  
Keyword(s):  
Soybean Cyst Nematode ◽  
Gene Families ◽  
Nematode Resistance ◽  
Cyst Nematode ◽  
Soybean Breeding ◽  
Resistant Varieties ◽  
Soybean Genotypes ◽  
Glycine Max L ◽  
Scn Resistance

Soybean cyst nematode (SCN) is one of the most damaging pest of soybean. Discovery and characterization of the genes involved in SCN resistance are important in soybean breeding. Soluble NSF attachment protein (SNAP) genes are related to SCN resistance in soybean. SNAP genes include five gene families, and 2 haplotypes of exons 6 and 9 of SNAP18 are considered resistant to the SCN. In present study the haplotypes of GmSNAP18 were surveyed and chacterized in a total of 60 diverse soybean genotypes including Korean cultivars, landraces, and wild-types. The target region of exons 6 and 9 in GmSNAP18 region was amplified and sequenced to examine nucleotide variation. Characterization of 5 haplotypes identified in present study for the GmSNAP18 gene revealed two haplotypes as resistant, 1 as susceptible and two as novel. A total of twelve genotypes showed resistant haplotypes, and 45 cultivars were found susceptible. Interestingly, the two novel haplotypes were present in 3 soybean lines. The information provided here about the haplotypic variation of GmSNAP18 gene can be further explored for soybean breeding to develop resistant varieties.

scholarly journals Soybean Stem Colonization by Genotypes A and B of Cadophora gregata Increases with Increasing Population Densities of Heterodera glycines

Plant Disease ◽  
2006 ◽  
Vol 90 (10) ◽  
pp. 1297-1301 ◽  
Author(s):  
G. M. Tabor ◽  
G. L. Tylka ◽  
C. R. Bronson
Keyword(s):  
Population Density ◽  
Soybean Cyst Nematode ◽  
Heterodera Glycines ◽  
Cyst Nematode ◽  
Stem Rot ◽  
Population Densities ◽  
Brown Stem Rot ◽  
Phialophora Gregata ◽  
Soybean Genotypes ◽  
Genotype A

Growth chamber experiments were conducted to investigate whether parasitism by increasing population densities of Heterodera glycines, the soybean cyst nematode, increases the incidence and severity of stem colonization by the aggressive genotype A and the mild genotype B of Cadophora gregata (Phialophora gregata), causal agents of brown stem rot of soybeans. Soybean genotypes with three combinations of resistance and susceptibility to H. glycines and genotype A of C. gregata were inoculated with each genotype of C. gregata alone or each genotype with two population densities of H. glycines eggs, 1,500 or 10,000 per 100 cm3 of soil. Stems of two H. glycines-susceptible soybeans were more colonized by both aggressive and mild genotypes of C. gregata in the presence of high than in the presence of low H. glycines population density.

scholarly journals Heterodera glycines Infection Increases Incidence and Severity of Brown Stem Rot in Both Resistant and Susceptible Soybean

Plant Disease ◽  
2003 ◽  
Vol 87 (6) ◽  
pp. 655-661 ◽  
Author(s):  
G. M. Tabor ◽  
G. L. Tylka ◽  
J. E. Behm ◽  
C. R. Bronson
Keyword(s):  
Soybean Cyst Nematode ◽  
Heterodera Glycines ◽  
Cyst Nematode ◽  
Stem Rot ◽  
Growth Chamber ◽  
Susceptible Genotype ◽  
Brown Stem Rot ◽  
Soybean Cultivars ◽  
Phialophora Gregata ◽  
Soybean Genotypes

Growth chamber experiments were conducted to investigate whether parasitism by Heterodera glycines, the soybean cyst nematode, increases incidence and severity of brown stem rot (BSR) of soybean, caused by Phialophora gregata, in both resistant and susceptible soybean cultivars. Soybean genotypes with various combinations of resistance and susceptibility to both pathogens were inoculated with P. gregata alone or P. gregata plus H. glycines. In most tests of H. glycines-susceptible genotypes, incidence and severity of internal stem discoloration, characteristic of BSR, was greater in the presence than in the absence of H. glycines, regardless of susceptibility or resistance to BSR. There was less of an increasing effect of H. glycines on stem symptoms in genotypes resistant to both BSR and H. glycines; however, P. gregata colonization of these genotypes was increased. Stems of both a BSR-resistant and a BSR-susceptible genotype were colonized earlier by P. gregata in the presence than in the absence of H. glycines. Our findings indicate that H. glycines can increase the incidence and severity of BSR in soybean regardless of resistance or susceptibility to either pathogen.

High-efficiency induction of soybean hairy roots and propagation of the soybean cyst nematode

Planta ◽  
2000 ◽  
Vol 210 (2) ◽  
pp. 195-204 ◽  
Author(s):  
Hyeon-Je Cho ◽  
Stephen K. Farrand ◽  
Gregory R. Noel ◽  
Jack M. Widholm
Keyword(s):  
Hairy Roots ◽  
Soybean Cyst Nematode ◽  
High Efficiency ◽  
Cyst Nematode

Soybean Genotypes with Resistance to Races of Soybean Cyst Nematode 1

Crop Science ◽  
1985 ◽  
Vol 25 (6) ◽  
pp. 1073-1075 ◽  
Author(s):  
S. C. Anand ◽  
J. A. Wrather ◽  
C. R. Shumway
Keyword(s):  
Soybean Cyst Nematode ◽  
Cyst Nematode ◽  
Soybean Genotypes

Expression of Soybean Cyst Nematode Resistance in Transgenic Hairy Roots of Soybean

Crop Science ◽  
1999 ◽  
Vol 39 (6) ◽  
pp. 1680-1686 ◽  
Author(s):  
R. A. Narayanan ◽  
R. Atz ◽  
R. Denny ◽  
N. D. Young ◽  
D. A. Somers
Keyword(s):  
Hairy Roots ◽  
Soybean Cyst Nematode ◽  
Nematode Resistance ◽  
Cyst Nematode ◽  
Transgenic Hairy Roots ◽  
Soybean Cyst Nematode Resistance

scholarly journals Novel resistance strategies to soybean cyst nematode (SCN) in wild soybean

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Janice Kofsky ◽  
Hengyou Zhang ◽  
Bao-Hua Song
Keyword(s):  
Gene Pool ◽  
Soybean Cyst Nematode ◽  
Agronomic Traits ◽  
Glycine Soja ◽  
Wild Soybean ◽  
Cyst Nematode ◽  
Resistance Response ◽  
Soybean Cultivars ◽  
Scn Resistance ◽  
Cultivated Soybean

AbstractSoybean cyst nematode (SCN, Heterodera glycine Ichinohe) is the most damaging soybean pest worldwide and management of SCN remains challenging. The current SCN resistant soybean cultivars, mainly developed from the cultivated soybean gene pool, are losing resistance due to SCN race shifts. The domestication process and modern breeding practices of soybean cultivars often involve strong selection for desired agronomic traits, and thus, decreased genetic variation in modern cultivars, which consequently resulted in limited sources of SCN resistance. Wild soybean (Glycine soja) is the wild ancestor of cultivated soybean (Glycine max) and it’s gene pool is indisputably more diverse than G. max. Our aim is to identify novel resistant genetic resources from wild soybean for the development of new SCN resistant cultivars. In this study, resistance response to HG type 2.5.7 (race 5) of SCN was investigated in a newly identified SCN resistant ecotype, NRS100. To understand the resistance mechanism in this ecotype, we compared RNA seq-based transcriptomes of NRS100 with two SCN-susceptible accessions of G. soja and G. max, as well as an extensively studied SCN resistant cultivar, Peking, under both control and nematode J2-treated conditions. The proposed mechanisms of resistance in NRS100 includes the suppression of the jasmonic acid (JA) signaling pathway in order to allow for salicylic acid (SA) signaling-activated resistance response and polyamine synthesis to promote structural integrity of root cell walls. Our study identifies a set of novel candidate genes and associated pathways involved in SCN resistance and the finding provides insight into the mechanism of SCN resistance in wild soybean, advancing the understanding of resistance and the use of wild soybean-sourced resistance for soybean improvement.

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