scholarly journals Increased Hydrogen Peroxide After Traumatic Brain Injury Disrupts Phosphatidylinositol 4,5‐Bisphosphate Metabolism Causing Impaired Inward Rectifier Potassium Channel Function

2018 ◽  
Vol 32 (S1) ◽  
Author(s):  
Adrian M. Sackheim ◽  
Nuria Villalba ◽  
Adrian Bonev ◽  
Mark Nelson ◽  
Kalev Freeman
Keyword(s):  
Hydrogen Peroxide ◽  
Traumatic Brain Injury ◽  
Brain Injury ◽  
Potassium Channel ◽  
Inward Rectifier ◽  
Channel Function ◽  
Inward Rectifier Potassium Channel ◽  
Rectifier Potassium Channel

scholarly journals Pathologically Entangled: Brain trauma-evoked ROS imbalance disrupts Kir channel function in distant peripheral vessels

Function ◽  
2021 ◽  
Author(s):  
Nick Weir ◽  
Thomas A Longden
Keyword(s):  
Traumatic Brain Injury ◽  
Brain Injury ◽  
Potassium Channels ◽  
Vascular Function ◽  
Inward Rectifier ◽  
Brain Trauma ◽  
Channel Function ◽  
Inward Rectifier Potassium Channels ◽  
Kir Channel

Abstract A Perspective on "Traumatic Brain Injury Impairs Systemic Vascular Function Through Disruption of Inward-Rectifier Potassium Channels"

scholarly journals Traumatic Brain Injury Impairs Systemic Vascular Function Through Disruption of Inward-Rectifier Potassium Channels

Function ◽  
2021 ◽  
Author(s):  
Adrian M Sackheim ◽  
Nuria Villalba ◽  
Maria Sancho ◽  
Osama F Harraz ◽  
Adrian D Bonev ◽  
...  
Keyword(s):  
Traumatic Brain Injury ◽  
Brain Injury ◽  
Potassium Channels ◽  
Signaling Pathways ◽  
Inward Rectifier ◽  
Pla2 Activity ◽  
Functional Responses ◽  
Channel Function ◽  
Inward Rectifier Potassium Channels ◽  
Kir2.1 Channel

Abstract Trauma can lead to widespread vascular dysfunction, but the underlying mechanisms remain largely unknown. Inward-rectifier potassium channels (Kir2.1) play a critical role in the dynamic regulation of regional perfusion and blood flow. Kir2.1 channel activity requires phosphatidylinositol 4,5-bisphosphate (PIP2), a membrane phospholipid that is degraded by phospholipase A2 (PLA2) in conditions of oxidative stress or inflammation. We hypothesized that PLA2–induced depletion of PIP2 after trauma impairs Kir2.1 channel function. A fluid percussion injury model of traumatic brain injury (TBI) in rats was used to study mesenteric resistance arteries 24 hours after injury. The functional responses of intact arteries were assessed using pressure myography. We analyzed circulating PLA2, hydrogen peroxide (H2O2), and metabolites to identify alterations in signaling pathways associated with PIP2 in TBI. Electrophysiology analysis of freshly-isolated endothelial and smooth muscle cells revealed a significant reduction of Ba2+-sensitive Kir2.1 currents after TBI. Additionally, dilations to elevated extracellular potassium and BaCl2- or ML 133-induced constrictions in pressurized arteries were significantly decreased following TBI, consistent with an impairment of Kir2.1 channel function. The addition of a PIP2 analog to the patch pipette successfully rescued endothelial Kir2.1 currents after TBI. Both H2O2 and PLA2 activity were increased after injury. Metabolomics analysis demonstrated altered lipid metabolism signaling pathways, including increased arachidonic acid, and fatty acid mobilization after TBI. Our findings support a model in which increased H2O2-induced PLA2 activity after trauma hydrolyzes endothelial PIP2, resulting in impaired Kir2.1 channel function.

scholarly journals Identification by Differential Display of a Hypertonicity-inducible Inward Rectifier Potassium Channel Highly Expressed in Chloride Cells

1999 ◽  
Vol 274 (16) ◽  
pp. 11376-11382 ◽  
Author(s):  
Yoshiro Suzuki ◽  
Makoto Itakura ◽  
Masahide Kashiwagi ◽  
Nobuhiro Nakamura ◽  
Taizo Matsuki ◽  
...  
Keyword(s):  
Potassium Channel ◽  
Differential Display ◽  
Inward Rectifier ◽  
Chloride Cells ◽  
Inward Rectifier Potassium Channel ◽  
Rectifier Potassium Channel

scholarly journals Complex Structure and Regulation of Expression of the Rat Gene for Inward Rectifier Potassium Channel Kir7.1

2000 ◽  
Vol 275 (36) ◽  
pp. 28276-28284 ◽  
Author(s):  
Nobuhiro Nakamura ◽  
Yoshiro Suzuki ◽  
Yugo Ikeda ◽  
Michitaka Notoya ◽  
Shigehisa Hirose
Keyword(s):  
Potassium Channel ◽  
Complex Structure ◽  
Inward Rectifier ◽  
Regulation Of Expression ◽  
Inward Rectifier Potassium Channel ◽  
Rectifier Potassium Channel

A Cholesterol Dimer Stabilizes the Inactivated State of an Inward‐rectifier Potassium Channel

2022 ◽  
Author(s):  
Collin G. Borcik ◽  
Isaac R. Eason ◽  
Maryam Yekefellah ◽  
Reza Amani ◽  
Ruixian Han ◽  
...  
Keyword(s):  
Potassium Channel ◽  
Inward Rectifier ◽  
Inward Rectifier Potassium Channel ◽  
Rectifier Potassium Channel

A Cholesterol Dimer Stabilizes the Inactivated State of an Inward‐rectifier Potassium Channel

2022 ◽  
Author(s):  
Collin G. Borcik ◽  
Isaac R. Eason ◽  
Maryam Yekefellah ◽  
Reza Amani ◽  
Ruixian Han ◽  
...  
Keyword(s):  
Potassium Channel ◽  
Inward Rectifier ◽  
Inward Rectifier Potassium Channel ◽  
Rectifier Potassium Channel
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