Characterizing a Phosphoglycolate Phosphatase Ortholog in D. melanogaster

2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1
Author(s):  
William Pascal Dwyer
Keyword(s):  
Phosphoglycolate Phosphatase

Phosphoglycolate Phosphatase in Several Population Groups in Israel

1981 ◽  
Vol 31 (2) ◽  
pp. 89-92 ◽  
Author(s):  
R. Golan ◽  
J.B. Ben-Ezzer ◽  
A. Szeinberg
Keyword(s):  
Population Groups ◽  
Phosphoglycolate Phosphatase

scholarly journals Phosphoglycolate has profound metabolic effects but most likely no role in a metabolic DNA response in cancer cell lines

2019 ◽  
Vol 476 (4) ◽  
pp. 629-643 ◽  
Author(s):  
Isabelle Gerin ◽  
Marina Bury ◽  
Francesca Baldin ◽  
Julie Graff ◽  
Emile Van Schaftingen ◽  
...  
Keyword(s):  
Dna Damage ◽  
Cell Lines ◽  
Oxidative Dna Damage ◽  
Fold Increase ◽  
Metabolic Effects ◽  
Phosphoglycerate Mutase ◽  
Wild Type ◽  
Metabolic Disturbances ◽  
Phosphoglycolate Phosphatase ◽  
Damage Repair

Abstract Repair of a certain type of oxidative DNA damage leads to the release of phosphoglycolate, which is an inhibitor of triose phosphate isomerase and is predicted to indirectly inhibit phosphoglycerate mutase activity. Thus, we hypothesized that phosphoglycolate might play a role in a metabolic DNA damage response. Here, we determined how phosphoglycolate is formed in cells, elucidated its effects on cellular metabolism and tested whether DNA damage repair might release sufficient phosphoglycolate to provoke metabolic effects. Phosphoglycolate concentrations were below 5 µM in wild-type U2OS and HCT116 cells and remained unchanged when we inactivated phosphoglycolate phosphatase (PGP), the enzyme that is believed to dephosphorylate phosphoglycolate. Treatment of PGP knockout cell lines with glycolate caused an up to 500-fold increase in phosphoglycolate concentrations, which resulted largely from a side activity of pyruvate kinase. This increase was much higher than in glycolate-treated wild-type cells and was accompanied by metabolite changes consistent with an inhibition of phosphoglycerate mutase, most likely due to the removal of the priming phosphorylation of this enzyme. Surprisingly, we found that phosphoglycolate also inhibits succinate dehydrogenase with a Ki value of <10 µM. Thus, phosphoglycolate can lead to profound metabolic disturbances. In contrast, phosphoglycolate concentrations were not significantly changed when we treated PGP knockout cells with Bleomycin or ionizing radiation, which are known to lead to the release of phosphoglycolate by causing DNA damage. Thus, phosphoglycolate concentrations due to DNA damage are too low to cause major metabolic changes in HCT116 and U2OS cells.

scholarly journals Characteristics and Sequence of Phosphoglycolate Phosphatase from a Eukaryotic Green AlgaChlamydomonas reinhardtii

2001 ◽  
Vol 276 (49) ◽  
pp. 45573-45579 ◽  
Author(s):  
Tarlan G. Mamedov ◽  
Kensaku Suzuki ◽  
Kenji Miura ◽  
Ken-ichi Kucho ◽  
Hideya Fukuzawa
Keyword(s):  
Phosphoglycolate Phosphatase

scholarly journals Correction: Phosphoglycolate phosphatase is a metabolic proofreading enzyme essential for cellular function in Plasmodium berghei.

2019 ◽  
Vol 294 (44) ◽  
pp. 16479-16479
Author(s):  
Lakshmeesha Kempaiah Nagappa ◽  
Pardhasaradhi Satha ◽  
Thimmaiah Govindaraju ◽  
Hemalatha Balaram
Keyword(s):  
Plasmodium Berghei ◽  
Cellular Function ◽  
Phosphoglycolate Phosphatase

Phosphoglycolate Phosphatase Polymorphism: Gene Frequencies in Three Italian Samples

1986 ◽  
Vol 36 (5) ◽  
pp. 281-285 ◽  
Author(s):  
C. Santolamazza ◽  
A. Benincasa ◽  
R. Scozzari
Keyword(s):  
Gene Frequencies ◽  
Phosphoglycolate Phosphatase
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