scholarly journals Glycosylation of aquaporins in the freeze tolerant tree frog, Hyla chrysoscelis

2009 ◽  
Vol 23 (S1) ◽  
Author(s):  
Khadijeh Alnajjar ◽  
Ram Naresh Pandey ◽  
James Frisbie ◽  
David L. Goldstein
Keyword(s):  
Tree Frog ◽  
Hyla Chrysoscelis ◽  
Freeze Tolerant

scholarly journals Aquaglyceroporin expression and erythrocyte omoregulation in cultures from the freeze tolerant anuran, Cope's gray tree frog, Hyla chrysoscelis

2012 ◽  
Vol 26 (S1) ◽  
Author(s):  
Venkateshwar Mutyam ◽  
James Frisbie ◽  
David L Goldstein ◽  
Carissa M Krane
Keyword(s):  
Tree Frog ◽  
Hyla Chrysoscelis ◽  
Freeze Tolerant ◽  
Gray Tree Frog

scholarly journals Membrane trafficking of aquaglyceroporin HC‐3 in erythrocytes from the freeze tolerant anuran, Cope's gray treefrog, Hyla chrysoscelis

2013 ◽  
Vol 27 (S1) ◽  
Author(s):  
Elizabeth Claire Wetzel ◽  
Philip Nickell ◽  
Venkateshwar Mutyam ◽  
James Frisbie ◽  
David L Goldstein ◽  
...  
Keyword(s):  
Membrane Trafficking ◽  
Hyla Chrysoscelis ◽  
Freeze Tolerant ◽  
Gray Treefrog

Excretion and conservation of glycerol, and expression of aquaporins and glyceroporins, during cold acclimation in Cope's gray tree frog Hyla chrysoscelis

2007 ◽  
Vol 292 (1) ◽  
pp. R544-R555 ◽  
Author(s):  
Sarah L. Zimmerman ◽  
James Frisbie ◽  
David L. Goldstein ◽  
Jennifer West ◽  
Kevin Rivera ◽  
...  
Keyword(s):  
Cold Acclimation ◽  
Expression System ◽  
Plasma Concentrations ◽  
Water Channel ◽  
Tree Frog ◽  
Hyla Chrysoscelis ◽  
Water Reabsorption ◽  
Real Time Quantitative Pcr ◽  
Xenopus Oocyte Expression ◽  
Gray Tree Frog

Cope's gray tree frog Hyla chrysoscelis accumulates glycerol during cold acclimation. We hypothesized that, during this process, gray tree frogs adjust renal filtration and/or reabsorption rates to retain accumulated glycerol. During cold acclimation, plasma concentrations of glycerol rose >200-fold, to 51 mmol/l. Although fractional water reabsorption decreased, glomerular filtration rate (GFR) and, consequently, urine flow were <5% of warm levels, and fractional glycerol reabsorption increased. In contrast, dehydrated frogs increased fractional water reabsorption, decreased GFR, and did not accumulate glycerol. We hypothesized that expression of proteins from the aquaporin (AQP)/glyceroporin (GLP) family was associated with changing patterns of water and glycerol movement. We cloned the cDNA for three such proteins, quantified mRNA expression in nine tissues using real-time quantitative PCR, and functionally characterized them using a Xenopus oocyte expression system. HC-1, an AQP1-like water channel conferring low glycerol permeability, is expressed ubiquitously in warm- and cold-acclimated tissues. HC-2, a water channel most similar to AQP2, is primarily expressed in organs of osmoregulation. HC-3, which is most similar to AQP3, is functionally characterized as a GLP, with low permeability to water but high permeability to glycerol. Aspects of expression levels and functional characteristics varied between cold and warm conditions for each of the three AQPs, suggesting a complex pattern of involvement in osmoregulation related to thermal acclimation.

Power output of sound-producing muscles in the tree frogs Hyla versicolor and Hyla chrysoscelis

1999 ◽  
Vol 202 (22) ◽  
pp. 3225-3237 ◽  
Author(s):  
M. Girgenrath ◽  
R.L. Marsh
Keyword(s):  
Power Output ◽  
Maximum Power ◽  
Trunk Muscles ◽  
Total Power ◽  
Hyla Versicolor ◽  
Tree Frog ◽  
Hyla Chrysoscelis ◽  
Force Velocity ◽  
External Oblique

Sound-producing muscles provide the opportunity of studying the limits of power production at high contractile frequencies. We used the work loop technique to determine the power available from the external oblique muscles in two related species of North American gray tree frog, Hyla chrysoscelis and Hyla versicolor. These trunk muscles contract cyclically, powering high-intensity sound production in anuran amphibians. The external oblique muscles in H. chrysoscelis have an in vivo operating frequency of 40–55 Hz at 20–25 degrees C, whereas in H. versicolor these muscles contract with a frequency of 20–25 Hz at these temperatures. In vivo investigations have shown that these muscles use an asymmetrical sawtooth length trajectory (with a longer shortening phase compared with the lengthening phase) during natural cycles. To study the influence of this particular length trajectory on power output, we subjected the muscles to both sinusoidal and sawtooth length trajectories. In both species, the sawtooth trajectory yielded a significantly higher power output than the sinusoidal length pattern. The maximum power output during sawtooth cycles was similar in both species (54 W kg(−)(1) in H. chrysoscelis and 58 W kg(−)(1) in H. versicolor). These values are impressive, particularly at the operating frequencies and temperatures of the muscle. The sinusoidal length trajectory yielded only 60 % of the total power output compared with the sawtooth trajectory (34 W kg(−)(1) for H. chrysoscelis and 36 W kg(−)(1) for H. versicolor). The optimum cycle frequencies maximizing the power output using a sawtooth length pattern were approximately 44 Hz for H. chrysoscelis and 21 Hz for H. versicolor. These frequencies are close to those used by the two species during calling. Operating at higher frequencies, H. chrysoscelis maximized power at a strain amplitude of only 8 % compared with a value of 12 % in H. versicolor. These strains match those used in vivo during calling. The stimulus timing observed in vivo during calling was also similar to that yielding maximum power at optimal frequency in both species (6 ms and 8 ms before the start of shortening in H. chrysoscelis and H. versicolor, respectively). As expected, twitch duration in H. chrysoscelis is much shorter than that in H. versicolor (23 ms and 37 ms, respectively). There was a less remarkable difference between their maximum shortening velocities (V(max)) of 13.6 L(0)s(−)(1) in H. chrysoscelis and 11.1 L(0)s(−)(1) in H. versicolor, where L(0) is muscle length. The force-velocity curves are very flat, which increases power output. At the myofibrillar level, the flat force-velocity curves more than compensate for the lower peak isometric force found in these muscles. The data presented here emphasize the importance of incorporating in vivo variables in designing in vitro studies.

scholarly journals Cell swelling induced by water and glycerol gradients in hepatocytes from warm‐and cold‐acclimated Hyla chrysoscelis , a freeze‐tolerant amphibian

2013 ◽  
Vol 27 (S1) ◽  
Author(s):  
PonTi Tsou ◽  
Geetha Koneru ◽  
Luiz Finatti ◽  
Kara Bobka ◽  
James Frisbie ◽  
...  
Keyword(s):  
Cell Swelling ◽  
Hyla Chrysoscelis ◽  
Freeze Tolerant

Isolation and characterization of simple sequence repeat loci in the gray tree frog, Hyla chrysoscelis

Genome ◽  
1999 ◽  
Vol 42 (4) ◽  
pp. 676-680 ◽  
Author(s):  
J.D. Krenz ◽  
R.D. Semlitsch ◽  
H.C. Gerhardt ◽  
P.A. Mahoney
Keyword(s):  
Simple Sequence Repeat ◽  
Tree Frog ◽  
Hyla Chrysoscelis ◽  
Sequence Repeat ◽  
Isolation And Characterization ◽  
Simple Sequence ◽  
Gray Tree Frog
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