scholarly journals Protein phosphatase 1 Inhibitor‐1 deficiency causes hypotension and reduces the phosphorylation of renal NaCl cotransporter (892.18)

2014 ◽  
Vol 28 (S1) ◽  
Author(s):  
Nicolas Picard ◽  
Katja Trompf ◽  
Chao‐Ling Yang ◽  
R. Miller ◽  
Monique Carrel ◽  
...  
Keyword(s):  
Protein Phosphatase ◽  
Protein Phosphatase 1 ◽  
Nacl Cotransporter

scholarly journals Protein Phosphatase 1 Inhibitor–1 Mediates the cAMP-Dependent Stimulation of the Renal NaCl Cotransporter

2019 ◽  
Vol 30 (5) ◽  
pp. 737-750 ◽  
Author(s):  
David Penton ◽  
Sandra Moser ◽  
Agnieszka Wengi ◽  
Jan Czogalla ◽  
Lena Lindtoft Rosenbaek ◽  
...  
Keyword(s):  
Protein Phosphatase ◽  
Mdck Cells ◽  
Ex Vivo ◽  
Phosphorylation Site ◽  
Mouse Kidney ◽  
Protein Phosphatase 1 ◽  
Endocrine Disorders ◽  
Kidney Slices ◽  
Nacl Cotransporter ◽  
Stimulation Of

BackgroundA number of cAMP-elevating hormones stimulate phosphorylation (and hence activity) of the NaCl cotransporter (NCC) in the distal convoluted tubule (DCT). Evidence suggests that protein phosphatase 1 (PP1) and other protein phosphatases modulate NCC phosphorylation, but little is known about PP1’s role and the mechanism regulating its function in the DCT.MethodsWe used ex vivo mouse kidney preparations to test whether a DCT-enriched inhibitor of PP1, protein phosphatase 1 inhibitor–1 (I1), mediates cAMP’s effects on NCC, and conducted yeast two-hybrid and coimmunoprecipitation experiments in NCC-expressing MDCK cells to explore protein interactions.ResultsTreating isolated DCTs with forskolin and IBMX increased NCC phosphorylation via a protein kinase A (PKA)–dependent pathway. Ex vivo incubation of mouse kidney slices with isoproterenol, norepinephrine, and parathyroid hormone similarly increased NCC phosphorylation. The cAMP-induced stimulation of NCC phosphorylation strongly correlated with the phosphorylation of I1 at its PKA consensus phosphorylation site (a threonine residue in position 35). We also found an interaction between NCC and the I1-target PP1. Moreover, PP1 dephosphorylated NCC in vitro, and the PP1 inhibitor calyculin A increased NCC phosphorylation. Studies in kidney slices and isolated perfused kidneys of control and I1-KO mice demonstrated that I1 participates in the cAMP-induced stimulation of NCC.ConclusionsOur data suggest a complete signal transduction pathway by which cAMP increases NCC phosphorylation via a PKA-dependent phosphorylation of I1 and subsequent inhibition of PP1. This pathway might be relevant for the physiologic regulation of renal sodium handling by cAMP-elevating hormones, and may contribute to salt-sensitive hypertension in patients with endocrine disorders or sympathetic hyperactivity.

scholarly journals The β‐adrenergic stimulation of the renal NaCl cotransporter is mediated via a cAMP‐dependent activation of protein phosphatase 1 inhibitor 1

2018 ◽  
Vol 32 (S1) ◽  
Author(s):  
David Penton Ribas ◽  
Sandra Moser ◽  
Agnieszka Wengi ◽  
Jan Czogalla ◽  
Lena Lindtoft Rosenbaek ◽  
...  
Keyword(s):  
Protein Phosphatase ◽  
Protein Phosphatase 1 ◽  
Adrenergic Stimulation ◽  
Nacl Cotransporter ◽  
Stimulation Of

scholarly journals Protein Phosphatase 1 Inhibitor-1 Deficiency Reduces Phosphorylation of Renal NaCl Cotransporter and Causes Arterial Hypotension

2013 ◽  
Vol 25 (3) ◽  
pp. 511-522 ◽  
Author(s):  
Nicolas Picard ◽  
Katja Trompf ◽  
Chao-Ling Yang ◽  
R. Lance Miller ◽  
Monique Carrel ◽  
...  
Keyword(s):  
Protein Phosphatase ◽  
Protein Phosphatase 1 ◽  
Arterial Hypotension ◽  
Nacl Cotransporter

Regulation of glycogen synthase and protein phosphatase-1 by hexosamines

Diabetes ◽  
1996 ◽  
Vol 45 (3) ◽  
pp. 322-327 ◽  
Author(s):  
E. D. Crook ◽  
D. A. McClain
Keyword(s):  
Protein Phosphatase ◽  
Glycogen Synthase ◽  
Protein Phosphatase 1
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