Suicide Gene/Prodrug Therapy for Pancreatic Adenocarcinoma by E. Coli Purine Nucleoside Phosphorylase and 6-Methylpurine 2′-deoxyriboside

Pancreas ◽  
2004 ◽  
Vol 28 (2) ◽  
pp. e54-e64 ◽  
Author(s):  
Sophie Deharvengt ◽  
Séverine Wack ◽  
Muriel Uhring ◽  
Marc Aprahamian ◽  
Amor Hajri
Keyword(s):  
Pancreatic Adenocarcinoma ◽  
Purine Nucleoside Phosphorylase ◽  
Suicide Gene ◽  
Purine Nucleoside ◽  
Nucleoside Phosphorylase ◽  
E Coli

scholarly journals Radical Dehalogenation and Purine Nucleoside Phosphorylase E. coli: How Does an Admixture of 2′,3′-Anhydroinosine Hinder 2-fluoro-cordycepin Synthesis

Biomolecules ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 539
Author(s):  
Alexey L. Kayushin ◽  
Julia A. Tokunova ◽  
Ilja V. Fateev ◽  
Alexandra O. Arnautova ◽  
Maria Ya. Berzina ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Chemical Synthesis ◽  
Purine Nucleoside Phosphorylase ◽  
Purine Nucleoside ◽  
Preparative Synthesis ◽  
Nucleoside Phosphorylase ◽  
E Coli ◽  
First Time

During the preparative synthesis of 2-fluorocordycepin from 2-fluoroadenosine and 3′-deoxyinosine catalyzed by E. coli purine nucleoside phosphorylase, a slowdown of the reaction and decrease of yield down to 5% were encountered. An unknown nucleoside was found in the reaction mixture and its structure was established. This nucleoside is formed from the admixture of 2′,3′-anhydroinosine, a byproduct in the preparation of 3-′deoxyinosine. Moreover, 2′,3′-anhydroinosine forms during radical dehalogenation of 9-(2′,5′-di-O-acetyl-3′-bromo- -3′-deoxyxylofuranosyl)hypoxanthine, a precursor of 3′-deoxyinosine in chemical synthesis. The products of 2′,3′-anhydroinosine hydrolysis inhibit the formation of 1-phospho-3-deoxyribose during the synthesis of 2-fluorocordycepin. The progress of 2′,3′-anhydroinosine hydrolysis was investigated. The reactions were performed in D2O instead of H2O; this allowed accumulating intermediate substances in sufficient quantities. Two intermediates were isolated and their structures were confirmed by mass and NMR spectroscopy. A mechanism of 2′,3′-anhydroinosine hydrolysis in D2O is fully determined for the first time.

scholarly journals In vivo sensitization of ovarian tumors to chemotherapy by expression of E. coli purine nucleoside phosphorylase in a small fraction of cells

Gene Therapy ◽  
2000 ◽  
Vol 7 (20) ◽  
pp. 1738-1743 ◽  
Author(s):  
V K Gadi ◽  
S D Alexander ◽  
J E Kudlow ◽  
P Allan ◽  
W B Parker ◽  
...  
Keyword(s):  
Purine Nucleoside Phosphorylase ◽  
Ovarian Tumors ◽  
Purine Nucleoside ◽  
Nucleoside Phosphorylase ◽  
E Coli
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