A Sensitive HPLC Method for the Determination of Fluoxetine and Norfluoxetine in Human Plasma with Fluorescence Detection

2005 ◽  
Vol 27 (1) ◽  
pp. 38-43 ◽  
Author(s):  
Sdka Ert??rk ◽  
Sevil M??ge ??etin ◽  
Sedef Atmaca ◽  
Lale Ersoy ◽  
G??l Baktr
2006 ◽  
Vol 29 (3) ◽  
pp. 403-414 ◽  
Author(s):  
Chong‐Kook Kim ◽  
Jun‐Pil Jae ◽  
Hye‐Ryung Hwang ◽  
Eunmi Ban ◽  
Jeong‐Eun Maeng ◽  
...  

2013 ◽  
Vol 96 (5) ◽  
pp. 987-990
Author(s):  
Sevgi Tatar Ulu

Abstract A sensitive HPLC method for the determination of methoxamine in human plasma and urine is described. The method is based on derivatization of methoxamine with 4-chloro-7-nitrobenzofurazan in borate buffer of pH 9.5 to yield a yellow, fluorescent product. Isocratic HPLC separation was achieved on an Inertsil C18 column (250 × 4.6 mm id, 5 μm particle size) using the mobile phase methanol–water (60 + 40, v/v) at a flow rate of 1.2 mL/min. Fluorescence detection was used at excitation and emission wavelengths of 458 and 521 nm, respectively. The assay was linear over the concentration ranges of 10–250 and 20–300 ng/mL for plasma and urine, respectively. The LOD values were 3.3 and 6.8 ng/mL and the LOQ values were 10 and 20 ng/mL for plasma and urine, respectively. The extraction recoveries were more than 97.10%. After strict validation, the method indicated good performance in terms of linearity, sensitivity, precision, accuracy (recovery), robustness, and system suitability, and it was successfully applied to the determination of methoxamine in human plasma and urine.


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