scholarly journals Evaluations of Auto-Focusing Methods under a Microscopic Imaging Modality for Metaphase Chromosome Image Analysis

2013 ◽  
Vol 36 (1-2) ◽  
pp. 37-44 ◽  
Author(s):  
Yuchen Qiu ◽  
Xiaodong Chen ◽  
Yuhua Li ◽  
Wei R. Chen ◽  
Bin Zheng ◽  
...  
Keyword(s):  
Bone Marrow ◽  
High Throughput ◽  
Metaphase Chromosome ◽  
Imaging Modality ◽  
Digital Pathology ◽  
Chromosome Analysis ◽  
Blood Specimens ◽  
Time Accuracy ◽  
High Throughput Imaging ◽  
Auto Focusing

Background: Auto-focusing is an important operation in high throughput imaging scanning. Although many auto-focusing methods have been developed and tested for a variety of imaging modalities, few investigations have been performed on the selection of an optimal auto-focusing method that is suitable for the pathological metaphase chromosome analysis under a high resolution scanning microscopic system.Objective: The purpose of this study is to investigate and identify an optimal auto-focusing method for the pathological metaphase chromosome analysis.Methods: In this study, five auto-focusing methods were applied and tested using metaphase chromosome images acquired from bone marrow and blood specimens. These methods were assessed by measuring a number of indices including execution time, accuracy, number of false maxima, and full width at half maximum (FWHM).Results: For the specific condition investigated in this study, the results showed that the Brenner gradient and threshold pixel counting methods were the optimal methods for acquiring high quality metaphase chromosome images from the bone marrow and blood specimens, respectively.Conclusions: Selecting an optimal auto-focusing method depends on the specific clinical tasks. This study also provides useful information for the design and implementation of the high throughput microscopic image scanning systems in the future digital pathology.

scholarly journals Interphase Chromosome Profiling: A Method for Conventional Banded Chromosome Analysis Using Interphase Nuclei

2017 ◽  
Vol 142 (2) ◽  
pp. 213-228 ◽  
Author(s):  
Ramesh Babu ◽  
Daniel L. Van Dyke ◽  
Vaithilingam G. Dev ◽  
Prasad Koduru ◽  
Nagesh Rao ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Peripheral Blood ◽  
Metaphase Chromosome ◽  
Spectral Characteristics ◽  
Chromosome Analysis ◽  
Artificial Chromosome ◽  
Turnaround Time ◽  
Interphase Nuclei ◽  
Interphase Chromosome

Context.— Chromosome analysis on bone marrow or peripheral blood samples fails in a small proportion of attempts. A method that is more reliable, with similar or better resolution, would be a welcome addition to the armamentarium of the cytogenetics laboratory. Objective.— To develop a method similar to banded metaphase chromosome analysis that relies only on interphase nuclei. Design.— To label multiple targets in an equidistant fashion along the entire length of each chromosome, including landmark subtelomere and centromere regions. Each label so generated by using cloned bacterial artificial chromosome probes is molecularly distinct with unique spectral characteristics, so the number and position of the labels can be tracked to identify chromosome abnormalities. Results.— Interphase chromosome profiling (ICP) demonstrated results similar to conventional chromosome analysis and fluorescence in situ hybridization in 55 previously studied cases and obtained useful ICP chromosome analysis results on another 29 cases in which conventional methods failed. Conclusions.— ICP is a new and powerful method to karyotype peripheral blood and bone marrow aspirate preparations without reliance on metaphase chromosome preparations. It will be of particular value for cases with a failed conventional analysis or when a fast turnaround time is required.

Complementarity of interphase and metaphase chromosome analysis in human renal tumors

1993 ◽  
Vol 6 (1) ◽  
pp. 17-23 ◽  
Author(s):  
Sandra R. Wolman ◽  
Frederic M. Waldman ◽  
Margit Balazs
Keyword(s):  
Metaphase Chromosome ◽  
Chromosome Analysis ◽  
Renal Tumors

scholarly journals High-throughput imaging of fresh-frozen plant reproductive samples in a variable pressure SEM

MethodsX ◽  
2021 ◽  
pp. 101392
Author(s):  
Haydee E. Laza ◽  
Bo Zhao ◽  
Mary Hastert ◽  
Paxton Payton ◽  
Junping Chen
Keyword(s):  
High Throughput ◽  
Variable Pressure ◽  
Fresh Frozen ◽  
High Throughput Imaging

Utility of High-Throughput Imaging Mass Cytometry for Cancer Research: A feasibility study

2021 ◽  
Author(s):  
Sindhura Thirumal ◽  
Amoon Jamzad ◽  
Tiziana Cotechini ◽  
Charles T. Hindmarch ◽  
Celine Hardy ◽  
...  
Keyword(s):  
Cancer Research ◽  
Feasibility Study ◽  
High Throughput ◽  
Mass Cytometry ◽  
High Throughput Imaging

Sex chiniaerism and germ cell distribution in a series of chimaeric mice

Development ◽  
1975 ◽  
Vol 33 (1) ◽  
pp. 205-216
Author(s):  
Anne McLaren
Keyword(s):  
Bone Marrow ◽  
Blood Cells ◽  
Chromosome Analysis ◽  
Chromosome Morphology ◽  
Mixed Population ◽  
Cell Distribution ◽  
Single Sex ◽  
Xx Males

1. Of 30 mice born from aggregation of embryos from a multiple recessive strain with F1 embryos carrying the contrasting alleles, 4 females and 20 males proved to be overtly chimaeric. 2. Three XX/XX females, five XY/XY males and eight XY/XX males were identified by chromosome analysis. Thus 50 % of the population analysed were sex chimaeras, and all of these developed as phenotypic males, though one showed evidence of hermaphroditism. 3. In seven XY/XX chimaeras that bred, the genetic component undergoing spermatogenesis coincided in every case with the component identified by chromosome morphology as XY. 4. The F1 component predominated in metaphase plates derived from cultured blood cells. Comparison with direct preparations from bone marrow suggested selection in favour of F1 cells, either through differential proliferation of stem cells in vivo or differential response to phytohaemagglutinin in vitro. 5. In XY/XX males, the percentage of XX cells detected varied from 1 % to 98 % in blood, and from 0 % to 80 % in bone marrow. 6. Of eight ‘single-sex’ chimaeras progeny-tested (three XX/XX, five XY/XY), only one showed evidence of a mixed population of germ cells. The proportion of the two types of progeny varied significantly from litter to litter, but was unrelated to the age of the male.

scholarly journals High-throughput imaging surface plasmon resonance biosensing based on ultrafast two-point spectral-dip tracking scheme

2020 ◽  
Vol 28 (14) ◽  
pp. 20624
Author(s):  
Youjun Zeng ◽  
Xueliang Wang ◽  
Jie Zhou ◽  
Ruibiao Miyan ◽  
Junle Qu ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
High Throughput ◽  
Plasmon Resonance ◽  
Imaging Surface Plasmon Resonance ◽  
High Throughput Imaging
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