Silk Suture Granuloma 37 Years After Scleral Buckle Surgery

Abstract: Introduction: Research has made progress in organ fabrication using an extracellular matrix, cell sheets, or organoids. Human liver tissue has been constructed using a 3-dimensional (3D) bioprinter and showed evidence that an in vitro generated liver bud was reformed in a rodent liver model. This study describes the stages of development of rat fetal organs and liver structure and reviews recent progress in liver organoid transplantation. Methods: The authors developed the procedures for creating a transected plane for use in experimental microsurgery in rats. A liver lobe was fixed vertically with gauze and it was ligated with 6-0 silk suture in the cut line; the parenchyma was cut, and major vessels were ligated to create the transected plane. The ligated tissue was carefully resected. Hemostasis was not required and hepatic components remained on the transected plane. The plane was covered by omentum. Results: Using this model, we transplanted fetal liver or a 3D bioprinted liver organoid. This microsurgical method enabled creation of an intact liver parenchyma plane. No bleeding was observed. The transplanted liver components successfully engrafted on the liver. Conclusion: This method may provide an essential environment for growing liver using portal and arterial blood flow.

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Clonidine as an Adjuvant to Prolong Local Analgesia in Conventional Scleral Buckle Surgery

Journal of Ocular Pharmacology and Therapeutics ◽

10.1089/jop.2013.0243 ◽

2014 ◽

Vol 30 (9) ◽

pp. 777-782 ◽

Cited By ~ 2

Author(s):

Marzena Górniak ◽

Johannes H. Proost ◽

Marc Veckeneer ◽

Verena C. Mulder ◽

René J. Wubbels

Keyword(s):

Local Analgesia ◽

Scleral Buckle

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Preoperative silk suture embolization of cerebral and dural arteriovenous malformations

Neurosurgical FOCUS ◽

10.3171/foc.2001.11.5.7 ◽

2001 ◽

Vol 11 (5) ◽

pp. 1-6 ◽

Cited By ~ 13

Author(s):

Amir R. Dehdashti ◽

Michel Muster ◽

Alain Reverdin ◽

Nicolas De Tribolet ◽

Daniel A. Ruefenacht

Keyword(s):

Arteriovenous Malformations ◽

Histopathological Examination ◽

Adverse Outcomes ◽

Neurological Deficits ◽

Grading System ◽

Silk Suture ◽

Long Term Follow Up ◽

Feeding Vessels ◽

A Minor ◽

Proximal Occlusion

Object The aim of this study was to evaluate the use of silk sutures as a medical implant when applied for the embolization of cerebral and dural arteriovenous malformations (AVMs). The facility of surgery and the clinical significance of complications related to preoperative silk suture embolization were evaluated immediately after surgery and at long-term follow up. Methods Thirty-four patients harboring 29 cerebral and five dural AVMs underwent embolization in which silk alone or in association with other agents was used. Medical and radiological records obtained in these 34 patients were reviewed retrospectively. The cerebral AVMs were classified according to the Spetzler–Martin grading system and the dural AVMs to the Djindjian grading system. The facility of the resection and the adverse outcomes, including new neurological deficits, hemorrhage, and fever, as well as histopathological evidence of vessel inflammatory changes, were determined in each case. In all 23 surgical cases, the AVM could be easily manipulated and excised. New temporary neurological deficits occurred in three patients. A high Spetzler–Martin grade was not associated with a higher incidence of new neurological deficits. One delayed-onset hemorrhage was detected after embolization. Fever was present in 24% of the patients. No sign of significant vasculitis or perivascular inflammation was found on radiological or histopathological examination. Conclusions Silk sutures are safe embolic agents especially for proximal occlusion of AVM feeding vessels. New permanent neurological deficits were not encountered in this series. Fever was considered to be a minor, temporary side effect of silk suture embolization.

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